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Supelco Presentations from Chirality 2009
21st International Symposium on Chirality
July 12-15, 2009
Beaver Run Resort and Conference Center
Breckenridge, Colorado, USA
www.chirality2009.org
The vendor seminar and poster presentations can now be viewed on-line - just click view presentation! |
| Vendor Seminar:
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Technical Poster Presentations:
- Significant Improvements in Chiral Method Development Using an LC-MS-Based Screening Approach (read abstract / view presentation - 544kb PDF)
- Performance and Benefits of Macrocyclic Glycopeptide-based CSPs in Enantiomeric Purification Using Bench-top Simulated Moving Bed (SMB) Technology (read abstract / view presentation 472kb PDF)
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| ABSTRACTS |
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| Strategies for Chiral HPLC Method Development (Vendor Seminar) |
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Authors: David S. Bell, Craig R. Aurand, Jennifer E. Claus, Jay M. Jones, Daniel Shollenberger
Presenter: Jennifer E. Claus
Date: Tuesday, July 14, 2009
Time: 12:30 PM – 1:30 PM, Refreshments will be served.
Abstract:
The retention processes that govern chiral separations are fascinating, but complex. This complexity leads to a time-consuming trial-and-error approach to method development that is often unproductive. Systematic studies on the effect of mobile phase conditions (in the case of chiral LC) and other analysis parameters can shed some light on the underlying mechanisms and be applied to streamline chiral method development and optimization. In this talk, we will discuss the impact of key variables, such as ion concentration, acid/base ratios and modifier type, on enantiomeric selectivity for a wide range of chiral compounds. The results of the study provide an improved understanding of the impact these variables have on chiral selectivity and thus offer a means to facilitate method development as well as unveil some of the underlying retention mechanisms responsible for retention and selectivity. The importance of mobile phase flexibility to three key chiral areas will be highlighted: polar/ionic compounds, LC-MS-compatibility, and preparative chiral separations. |
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| Significant Improvements in Chiral Method Development Using an LC-MS-Based Screening Approach (Poster Presentation) |
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Authors: David S. Bell, Jennifer E. Claus, Jay M. Jones
Abstract:
The highly sensitive and complex retention mechanisms associated with chiral chromatography often lead to challenges in method development. Problems such as retention time instability and irreproducibility may hamper the development of robust chiral HPLC methods. Macrocyclic glycopeptide and cyclodextrin-based chiral stationary phases (CSPs) often provide enantiomeric selectivity using polar organic and aqueous-organic mobile phases. As these mobile phases are amenable to mass spectrometry (MS) sources, LC-MS techniques may be utilized to run selectivity studies for a large variety of compounds in a short period of time. In this study, the impact of mobile phase composition was examined to develop stable, robust, and reproducible methods for the analysis of various pharmaceutically-relevant compounds. The results of the study provide an improved understanding of the influence these variables have on chiral selectivity and thus offer a means to facilitate method development. The impact of the important variables in method development protocols is highlighted. |
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Performance and Benefits of Macrocyclic Glycopeptide-based CSPs in Enantiomeric Purification
Using Bench-top Simulated Moving Bed (SMB) Technology (Poster Presentation) |
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Authors: J.T. Lee and William Campbell (Supelco, Division of Sigma-Aldrich); Robert Mierendorf, SEMBA Biosciences
Abstract:
Chiral stationary phases (CSPs) comprising macrocyclic glycopeptides covalently bonded to high purity silica have significant benefits in preparative chiral separations, including wide enantioselectivity, use of mobile phases that can be optimized for maximum sample solubility, ability to retain and resolve polar and ionic compounds, and excellent robustness. When used in conjunction with a new bench-top simulated moving bed (SMB) instrument, the benefits of these CSPs are combined with the advantages of SMB for the rapid isolation of gram quantities of purified enantiomers.
This presentation will demonstrate the power of macrocyclic glycopeptide CSPs and the aforementioned bench-top SMB system to purify chiral pharmaceutical compounds in polar mobile phase systems that enhance the compound’s solubility. The advantages of this approach over traditional single column batch-type preparative chromatography will be shown in terms of productivity, reduction of solvent waste and product recovery. The ease of method development from HPLC to SMB scales and the ruggedness of the CSPs and the instrument will also be demonstrated. |
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