Abstracts

Special Topics, Schedule for 2011 - Jan | Feb | Mar | Apr | May | Jun | Jul | Aug | Sep | Oct | Nov | Dec
Special Topics, Schedule for 2010
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Presenter Profiles

Technical Session Abstracts

LC, LC-MS and Sample Prep for LC: Techniques to Improve Speed, Resolution, and Sensitivity without Investing in Capital Equipment
This talk describes recent innovations in particles, sorbents, surface chemistries, column/device formats, and reagents for HPLC and sample prep that address the need for higher throughput, better sensitivity, and greater resolution. Intended to be instructional, the talk will explain the scientific principles behind today’s innovative technologies, such as Fused-Core^® particles, HybridSPE™-Phospholipid technology for the low-level MS detection of small molecules in serum, solid phase microextraction (SPME), which has now been applied to bioanalysis, molecular imprinted polymer SPE (SupelMIP™), and others. Applications will be presented that demonstrate the utility of these innovations in pharma, food, forensics, environmental, bioanalysis, and other areas using interesting case studies based on the registrants’ areas of interest.

GC, GC-MS and Sample Prep for GC: Techniques to Improve Speed, Resolution, and Sensitivity without Investing in Capital Equipment
Analysts interested in GC, GC-MS, and/or sample prep techniques that employ a GC finish will find this talk valuable. Intended to be instructional, this talk describes the scientific principles behind today’s innovative technologies. Topics include ionic liquids, which show the promise of revolutionizing capillary GC because they offer the benefits of polarity/selectivity without the penalty of low temperature operation, ways to increasing throughput using Fast GC, solid phase microextraction (SPME), unique carbon adsorbents, QuEChERS, Ag-ion SPE for FAMEs, and others. Applications will be presented that demonstrate these innovations in pharma, food, forensics, environmental, bioanalysis, and other areas using interesting case studies geared toward the registrants’ areas of interest.

Special Topics in Chromatography and Sample Prep
In this segment we will use special guest speakers to introduce current chromatography and sample prep topics. Title and speaker will be announced closer to the time of the event. Please consult the web site for up-to-date information.

Special Topics, Schedule for 2011

January 11, Sherman Oaks, CA
January 12, Irvine, CA
January 13, San Diego, CA

(Both topics are presented)

Advances in Macrocyclic Chiral Stationary Phases for LC & SFC Enantiomeric Separations
Presenter: Prof. Daniel W. Armstrong, Robert A. Welch Professor of Chemistry at the University of Texas at Arlington, Arlington, TX
Abstract:
Chiral macrocyclic molecules are one of the most important classes of chiral selectors known. They dominate GC and CE enantiomeric separations, as well as reversed phase and polar organic mode LC separations. This class of selectors will be briefly reviewed and the latest exciting results presented. The newest and most unique member of this family, cyclofructans, will be introduced. If time allows, new chiral stationary phases based on synthetic chiral polymers will be discussed as well.

Ionic Liquids in GC (Including Rapid Water Analyses) and the Ultra-Sensitive ESI-MS of Anions
Presenter: Prof. Daniel W. Armstrong, Robert A. Welch Professor of Chemistry at the University of Texas at Arlington, Arlington, TX
Abstract:
The first new class of GC stationary phases in 30 years are based on ionic liquids (ILs). They have unique selectivities, higher thermal and chemical stabilities than any of the known commercially available columns. We will describe them and their use in: the rapid, facile water analysis of virtually any solvent and/or beverage, high temperature separations, FAME isomer separations, separations with an air carrier gas, GC x GC, and other applications. ILs with multifunctional cations also can be used as a trace additive for the ultra sensitive detection of anions in the positive mode of ESI-MS. The mechanism and use of this novel technique will be described for both organic and inorganic anions.

February 8, So. San Francisco, CA
February 9, Palo Alto, CA
February 10, Oakland / Berkeley, CA

Thinking Outside the Silica Framework: Alternate Supports for HPLC and When to Use Them
Presenter: Mark Carrier, Chromatography Market Segment Manager, Hamilton Company, Reno, NV
Abstract:
Although silica comprises the lion’s share of the HPLC particle market, synthetic polymer-based particles have important applications across many industries, including environmental, toxicology, and biopharma. The chemical nature of polymeric particles expands the working range of pH and mobile phases available to chromatographers. This talk is geared toward understanding the differences between silica and polymers as HPLC supports, and will focus on the benefits over traditional silica particles in pH range, chemical compatibility, and temperature range. The structures of the various polymeric supports, differences and advantages, will be discussed. Functionalization details of the different ion exchange material types will also be shown.

March 22, Houston, TX
March 23, San Antonio, TX
March 24, Dallas, TX

(Both topics are presented)

April 26, Omaha, NE
April 27, Kansas City, MO

Robust Particles for HPLC Provide Stability up to pH 12 and Quick Method Development in Peptide Purification
Presenter: Jared W. Benedict, Ph. D., Marketing and Sales Manager, AkzoNobel/Kromasil, Brewster, NY
Abstract:
Innovations that improve a HPLC silica particle’s robustness or expand its operating range are needed to allow chromatographers flexibility in method development. Researchers at AkzoNobel in Sweden have invented and applied some truly unique surface chemistry that expands the utility of silica particles far beyond what is typical for silica, while maintaining efficiency, retention and selectivity. Called “Kromasil Eternity”, this new generation material is stable from pH 1 to 12 in both HPLC and UHPLC applications. The presentation shows several applications that exemplify the wide applicability of this material. Due to the newly introduced partially integrated surface modification, no significant column degeneration was observed over an extended period of time at high pH. The new generation Kromasil HPLC silica material was also packed in semi-prep columns commonly used in combinatorial chemistry and development labs. Applications show how a pH affects loadability and chemist can purify a large amount of a basic compound in a method at pH 11.0. As a result of the combination of extended chemical stability and high column efficiency, challenging preparative separations of APIs were performed with reproducible results. The second part of the seminar will explain preparative chromatography and show a case study purifying a peptide at an R&D scale. When purifying a compound at a research level the most crucial factor is time. Secondarily, cost due to solvent usage or lost crude is considered. Therefore developing a method quickly for peptide purification in which the target quantity of peptide can be purified in an efficient way is a necessary tool to have in a R&D lab. This presentation will show how screening stationary phase, mobile phase, pH and then calculating productivity can be done quickly and produce a required result.

April 28, St. Louis, MO

High-Throughput Accurate Mass Screening of Monoclonal Antibodies
Presenter: Jim Blasberg, Senior Scientist, Sigma-Aldrich Chemical Co., St. Louis, MO
Abstract:
Post-translational modifications, particularly the structure of N-glycans, have been shown to affect bioactivity, immunogenicity and clearance rate of therapeutic glycoproteins. Here we report development and application of a rapid and high throughput analytical method using intact protein accurate mass data to perform glycoprofiling directly from cell culture supernatant. This data will be used for early clonal screening and selection as well as cell engineering to eliminate undesirable populations.

May 17, Toronto, ON
May 18, Montreal, QC
May 19, Ottawa, ON

Novel Applications with Ion exchange Chromatography for the Biopharmaceutical Industry
Presenter: Atis Chakrabarti, Ph.D., Technical Service Specialist, Tosoh Bioscience, Montgomeryville, PA
Abstract:
For over 20 years, Tosoh Bioscience has provided liquid chromatography products to the biopharmaceutical industry. In order to maintain our leadership role in providing these solutions, it is important to develop new products to meet the needs of an evolving and demanding industry. Tosoh Bioscience recently introduced the TSK-GEL STAT analytical product line for ion exchange analysis. These new prepacked columns feature a non-porous resin designed for rapid analysis, excellent selectivity, and increased capacity compared to traditional non-porous resins currently on the market. One particular application that these columns are ideally suited is the real time analysis of the PEGylation reaction of various proteins. The anion exchange version of these columns is also well suited for nucleic acid analysis. Data will be shown to demonstrate the versatility of these new columns.

May 24, Rockville, MD
May 25, Richmond, VA
May 26, RTP, NC

Part 1: Advances in Macrocyclic Chiral Stationary Phases for LC & SFC Enantiomeric Separations
Presenter: Zachary S. Breitbach, Ph.D., Research Engineering Associate, Dept. of Chemistry, University of Texas at Arlington, Arlington, TX
Abstract:
Chiral macrocyclic molecules are one of the most important classes of chiral selectors known. They dominate GC and CE enantiomeric separations, as well as reversed phase and polar organic mode LC separations. This class of selectors will be briefly reviewed and the latest exciting results presented. The newest and most unique member of this family, cyclofructans, will be introduced. If time allows, new chiral stationary phases based on synthetic chiral polymers will be discussed as well.

Part 2: Ionic Liquids in GC (Including Rapid Water Analyses) and the Ultra-Sensitive ESI-MS of Anions
Presenter: Zachary S. Breitbach, Ph.D., Research Engineering Associate, Dept. of Chemistry, University of Texas at Arlington, Arlington, TX
Abstract:
The first new class of GC stationary phases in 30 years are based on ionic liquids (ILs). They have unique selectivities, higher thermal and chemical stabilities than any of the known commercially available columns. We will describe them and their use in: the rapid, facile water analysis of virtually any solvent and/or beverage, high temperature separations, FAME isomer separations, separations with an air carrier gas, GC x GC, and other applications. ILs with multifunctional cations also can be used as a trace additive for the ultra sensitive detection of anions in the positive mode of ESI-MS. The mechanism and use of this novel technique will be described for both organic and inorganic anions.

June 14, N. Chicago, IL
June 15, Madison, WI
June 16, Minneapolis, MN

Innovations in Sample Preparation Utilizing Membrane-Entrapped Sorbent Particles for SPE: The 3M Empore Technology
Presenter: Jennifer Heitkamp, M.S., Technical Specialist, Technology Commercialization Group, 3M Purification, Inc., St. Paul, MN
Abstract:
3M’s Empore™ technology employs a unique process to entrap small absorbent particles into a matrix of PTFE fibrils creating a mechanically stable membrane. Although the technology has been used by analytical chemists for over 20 years, it is still applied to solve new sample prep challenges today. The membrane can be used in a disk format or can be put into cartridges or 96-well plates as needed. The membrane has a high exposed surface area of active particles. This characteristic property makes the membrane extremely useful for many applications that may not be typical in many laboratories. This talk will give some examples of ways that the Empore membrane format can be used to prepare samples of large volumes used in environmental analysis to very small volumes used in drug discovery.

July 19, Rochester, NY
July 20, Syracuse, NY
July 21, Albany, NY

July 25, Vancouver, BC
July 27, Calgary, AB
July 28, Edmonton, AB

Making Good Connections in Almost Any Application
Presenter: John Batts, Technical Specialist, IDEX Health & Science LLC, Oak Harbor, WA
Abstract:
With the evolution of chromatography and related techniques, never before has making good connections been so important to ensuring you receive the results you need from your analyses. This seminar will cover "the basics" of making good, reliable connections with your tubing and fittings. Then, we will discuss what special features must be addressed for applications like micro and nano-flow chromatography and UHPLC; the two areas that have received a lot of attention and are expanding the most rapidly. Time will be set aside for questions to ensure the topics that are most important to your applications are covered.

August 16, Denver, CO
August 18, Phoenix, AZ

September 13, Rahway, NJ

SPME: 20 Years Young
Presenter: Nicholas H. Snow, Ph.D., Department of Chemistry and Biochemistry, Center for Academic Industry Partnership, Seton Hall University, South Orange, NJ
Abstract:
First developed about 20 years ago, solid-phase microextraction (SPME) has become one of the most valuable sample preparation tools in separation science. In fact, the advent of SPME led to new and renewed attention to extraction techniques involving sorbents as the extraction medium. While we might consider a 20-year old technique mature, new extensions of SPME’s applications are continuously being developed. This presentation will trace the development of SPME over the nearly 20 years that it has been on the market, from beginnings with just PDMS fibers and manual injection to the range of fibers, configurations and automation possibilities available today. Applications will be traced from the original analysis of aromatics (BTEX) in water by direct immersion to headspace SPME, semi-volatile analysis and drug analysis both with and without derivatization of the drugs. Basic method development considerations will also be discussed. SPME continues to be one of the most versatile and straightforward to operate sample preparation techniques in separation science.

September 14, Princeton, NJ		Register for event
	*Development of Fast HPLC Methods: An Analytical Chemist’s Perspective* Presenter: Prabo Wijetunge, Ph.D., MicroDose Therapeutx, Monmouth Junction, NJ Abstract: Today's analytical chemist faces many challenges when developing fast HPLC methods. Limitations of time, money, instruments, and resources, as well as the drive for faster methods, puts us in the center of a difficult task. More than ever, the methods need to be rugged and transferable, as well as providing the necessary resolution. This presentation will address some of these challenges and demonstrate some case studies involving Fused-Core HPLC columns that help overcome some of the challenges we face.

September 15, Collegeville, PA		Register for event
	*Sample Preparation for Analysis of Small Organic Molecules Using the“Quick, Easy, Cheap, Effective, Rugged, and Safe” (QuEChERS) Approach* Presenter: Steven J. Lehotay, Ph.D., Lead Scientist, USDA Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA Abstract: The introduction of modern GC-MS and LC-MS techniques have had great impact on the ability to analyze organic molecules in complex matrices, and the monitoring of pesticides, veterinary drugs, environmental contaminants, and other chemicals of interest in foods has been no exception. However, the power of these advanced analytical tools is seriously diminished by sample matrix effects. The QuEChERS technique was developed to streamline sample preparation particularly for GC-MS and LC-MS analysis, and it has become a widely-used sample preparation approach to reduce matrix effects in a variety of matrices, especially food. In this talk, the speaker will describe QuEChERS concepts and newest developments and applications. He will also discuss some interesting associated analytical developments, including fast, low-pressure GC-MS(/MS), analyte protectants for GC analysis, and streamlined method validation.

October 18, Boston/Cambridge, MA
October 19, (see below)
October 20, Long Island, NY

October 19, Wallingford, CT

3:45 PM: Mechanism of Enantioseparation on Macrocyclic Glycopeptide Antibiotic Phases
Presenter: Shengli Ma, Ph.D., Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, CT
Abstract:
Macrocyclic glycopeptides are a class of compounds often used for separation of enantiomers via either HPLC or CZE. Two such macrocyclic glycopeptides are teicoplanin and teicoplanin aglycone, and our work focused on their chromatographic behavior. These compounds are immobilized on silica matrixes and are commercially available under the trade names Astec CHIROBIOTIC® T2 and TAG, respectively. The separation of propranolol was performed on both chiral stationary phases. A reversal of elution order was obtained when the compound was separated on CHIROBIOTIC T2, compared with TAG. In order to understand this behavior, vibrational circular dichroism was undertaken in different solvents and at different temperature. The presentation will describe all the changes occurring with these two macrocycles under the experimental conditions.

4:35 PM: Solid Phase Microextraction (SPME): Technology Overview and Recent Advances using SPME in Bioanalysis
Presenter: Hillel Brandes, Ph.D., Principle Applications Chemist, Supelco, Bellefonte, PA
Abstract:
This talk will describe a new Solid Phase Microextraction (SPME) fiber probe that permits the extraction of small molecule analytes directly from biological fluids and other complex matrixes. It comprises a metal-based fiber core coated with mixed-mode HPLC particles (C18/SCX) embedded in a proprietary biocompatible binder. Large macromolecules, such as proteins and glycolipids, are repelled, while smaller analytes, such as drugs and metabolites, bind to the particles and are extracted. Because SPME is concentration dependent rather than volume dependent, sample volumes down to 100 µL can be extracted and desorbed with as little as 50 µL. This is an advantage with precious samples, like plasma from neonates, transgenic mice, and other small mammals. The device is compatible with common laboratory robotics equipment, and allows simultaneous extraction or desorption of multiple samples for high throughput sample prep.

5:15 PM: Transfer of Biomarker Assays from RIA and ELISA to LC/MS/MS
Presenter: Kimberly Navetta, Pfizer, Groton Laboratories, Discovery Safety Evaluation
Abstract:
The Biomarkers’ Laboratories at Pfizer have initiated the replacement of some ELISA and RIA kits for small molecule biomarkers with mass spectrometry (MS) assays. When a direct comparison was conducted, the MS assays demonstrated three major benefits over the kits: lower costs, shorter delivery times, and higher levels of specificity. For example, the MS assay for aldosterone costs ten times less and data reports are generated 2 days faster. Corticosterone was moved to the MS assay not only for the cost savings, but the reduction in sample volume requirements. When evaluating the specificity of the kits for small molecules, many antibodies used cross-reacted with molecules of similar structures, potentially providing false readings. To date, three MS assays have been deployed to portfolio programs in place of their respective kits (corticosterone, aldosterone and 25-OH Vitamin D3), and other assays are being evaluated for future deployment. As MS is becoming established in the clinical setting, this platform is broadening its impact in clinical pathology applications, enhancing drug development. These changes not only provide substantial cost and time savings, but they provide higher quality data for project teams.

November 29, Birmingham, AL
November 30, Memphis, TN
December 1, Little Rock, AR

A Selectable 1D or 2D GC-MS System based on Capillary Flow Technology
Presenter: John R. Stuff, Ph.D., Senior Applications Chemist, GERSTEL, Inc. Linthicum, MD
Abstract:
Identification of important trace components in complex samples, like fragrances, natural products, petroleum fractions, or polymers, can be challenging. Achieving the mass on column and resolution necessary to locate and identify trace components using a single chromatographic separation can be difficult, if not impossible. A selectable 1D/2D GC/MS configuration using Agilent capillary flow technology (CFT) and low thermal mass (LTM) GC column modules with dissimilar column phases was used to perform heartcutting. The main advantages of this configuration is the simple selection of single or two dimensional operation and the ability to use the mass spectrometer as the monitoring detector for the first dimension and also for detection in the second dimension. Several examples of separation and identification of selected trace components from consumer products will be shown to demonstrate the effectiveness of this system. If additional mass is necessary to detect the analyte of interest in the second dimension separation, a cryotrap after the precolumn can function as a fraction collector to accumulate fractions from many replicates of the sample.

December 13, Knoxville, TN
December 14, Greenville, SC
December 15, Atlanta, GA

Innovative Particles and Column Technologies for the High Throughput Separation of Biomolecules: A Brief Overview
Presenter: Atis Chakrabarti, Ph.D., Technical Service Specialist, Tosoh Bioscience, Montgomeryville, PA
Abstract:
For over 20 years, Tosoh Bioscience has provided comprehensive lines of high and low pressure columns of different modes of chromatography to meet a variety of purification needs. In order to maintain our leadership role in providing these solutions, it is important to develop new products to meet the needs of an evolving and demanding industry. This talk will cover an overview of the innovations in surface chemistry and column technology from Tosoh in different modes of chromatography for the separation of biomolecules.

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