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Analytical / Chromatography > Food Safety > Bacteria & Natural Toxins
Food Safety

Bacteria & Natural Toxins

Foodborne Illness or food poisoning typically occurs from one or more of the following sources – viral, bacterial, and fungal contamination.


Bacterial Contamination
Natural Toxins – Fungal (Mycotoxins) & Algal (Phycotoxins)

Bacterial Contamination back to top

The most commonly known foodborne contaminants are E. coli O157:H7, Salmonella, and Campylobacter (Norwalk-like virus). Other diseases that can be transmitted via food channels from one human to another human via prepared foods are Hepatitis A, Shigella, Giardia lamblia, and Crytosporidia.

Bacteria occur naturally in food sources and may release toxins into the food or into the body after consumption if the conditions are ideal to stimulate bacterial growth. These toxins can be found at any stage in the food cycle from production and handling, to processing and storage. Examples of common bacterial toxins are Bacillus cereus. Food types commonly associated with bacterial toxins are bakery goods, cooked meats, deli products, gravy, boiled rice; Salmonella in uncooked meats.

Microbes in food and beverage applications are detected through microbiological screening methods and culturing. Our HybriScan™ Rapid Test System, organism detection strips identification kits and comprehensive media selection guide by organism or by food type are useful for microbial determinations.


Literature & References
USDA Fact Sheet – Foodborne Illness and Disease
FDA Bad Bug Book
USDA Food Safety and Inspection Service (FSIS) Methods:
   Main Site
   Analytical Chemistry Methods Guidebook
   Microbiology Methods Guidebook
   Pathology Guidebook
   Data Collection Reports (food)
Sigma Aldrich Microbiology Focus Newsletter
Sigma Aldrich Analytical Microbiology Guide (CD request)

Related Products
Microbiology Products
  Identification Tests & Reagents
  HybriScan Rapid Test Kits
  Differentiation and Identification Media for Salmonella: Microbiology Focus Newsletter Vol. 1.3 (pdf)
  Food & Beverage Microbiology


Natural Toxins – Fungal (Mycotoxins) & Algal (Phycotoxins) back to top

Some foods contain harmful toxins as a result of product spoilage, damage and mold growth. Examples of harmful toxins found in plant-based foods are lectins (red beans), glycolalkaloids (green potatoes), and patulin (damaged apples).

Harmful toxins from algae, known as algal toxins (Phycotoxins), are found in shellfish and are referred to as shellfish poisons – paralytic (PSP), amnesic (ASP) and diarrhetic (DSP).

Mycotoxins are produced from mold and other fungi, are invisible and can penetrate food matter. Even if suspected mold growth is removed from bread or cheese, it may still be present and should not be consumed. Aflatoxins, the more commonly found mycotoxins, are produced by Aspergillus and are found in cereal grains, oil seeds and tree nuts. Aflatoxins have received greater attention than any other mycotoxins because of their demonstrated potent carcinogenic effect in susceptible laboratory animals and their acute toxicological effects in humans. Aflatoxins are the most widely known and well researched. They are associated with aflatoxicosis (poisoning from ingestion) in livestock, domestic animals, and humans. The occurrence of aflatoxins is influenced by environmental factors such as pH and temperature; therefore, the degree of contamination will vary with geographic location, agricultural practice, and the susceptibility of foodstuff to fungal invasion during pre-harvest, storage, and/or processing stages.


Applications
SPE/HPLC Application: Aflatoxins from Cornmeal
HPLC Analysis: Common Analogs of Aflatoxin 
HPLC Analysis of Patulin in Apple Juice (pdf)

Literature & References
USDA Food Safety Research –Pathogens and Contaminants-Aflatoxins
USDA Fact Sheet – Molds on Food
EU Commission Regulation (EC) No 401/2006 of 23 February 2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs Text with EEA relevance

Related Products
HPLC Columns
  Ascentis C18, 15 cm × 4.6 mm I.D., 3 µm particles (581322-U)
  Ascentis C18, 25 cm × 4.6 mm I.D., 5 µm particles (581325-U)
  SUPELCOSIL LC-18, 25 cm x 4.6 mm ID, 5 µm particles (58298)
(In place of SUPELCOSIL™ C18, we recommend Ascentis® C18, based on newer technology)
SPE Tubes
  Supelclean LC-CN SPE Cartridge 500 mg/3 mL (57013)
Analytical Standards
  Mycotoxins
  Patulin solution, 100 µg/mL in chloroform (46914-U)