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SP™-2560 for detailed cis/trans fatty acid isomers as methyl esters
Omegawax™ for omega-3 and omega-6 fatty acids as methyl esters
Nukol™ for free fatty acids
Equity®-1 for boiling point elution of fatty acids as methyl esters
SP-2380 for cis/trans fatty acid isomers as methyl esters
SPB™-PUFA for polyunsaturated fatty acids (PUFAs) as methyl esters
- Application: This highly polar biscyanopropyl column was specifically designed for the separation of geometric-positional (cis/trans) isomers of fatty acid methyl esters (FAMEs). No other column is able to provide the same level of cis/trans detail. This column is specified in AOAC Method 996.06 and AOCS Method Ce 1h-05.
- USP Code: This column meets USP G5 requirements
- Polymer: Non-bonded; poly(biscyanopropyl siloxane)
- Temperature Limits: Subambient to 250 °C
- Ordering Information for: SP-2560
Chromatograms:
37-Component FAME Mix on the 75 m SP-2560
Detailed Analysis of C18 FAME Isomers on the 75 m SP-2560
37-Component FAME Mix on the 100 m SP-2560
Detailed Analysis of C18 FAME Isomers on the 100 m SP-2560
- Application: This column allows highly reproducible analyses of omega-3 and omega-6 fatty acids as methyl esters. All Omegawax columns are specially tested to ensure reproducible FAME equivalent chain length (ECL) values and resolution of key components. This column is specified in AOAC Method 991.39 and AOCS Method Ce 1b-89.
- USP Code: This column meets USP G16 requirements
- Polymer: Bonded; poly(ethylene glycol)
- Temperature Limits: 50 °C to 280 °C
- Ordering Information for: Omegawax
Chromatograms:
37-Component FAME Mix on the Omegawax 100
Marine Source FAMEs on the Omegawax 100
Menhaden Oil FAMEs on the Omegawax 100
Animal Source FAMEs on the Omegawax 100
37-Component FAME Mix on the Omegawax 250
Omegawax Test Mix on the Omegawax 320
- Application: The incorporation of acid functional groups into the phase lends an acidic character to this column, useful for analyses of volatile acidic compounds. Difficult to analyze carboxylic acids (free fatty acids) can be analyzed with excellent peak shape and minimal adsorption.
- USP Code: This column meets USP G25 and G35 requirements.
- Polymer: Bonded; acid-modified poly(ethylene glycol)
- Temperature Limits: 60 °C to 200/220 °C
- Ordering Information for: Nukol
Chromatograms:
Short Chain Free Fatty Acids on the Nukol
Short and Long Chain Free Fatty Acids on the Nukol
Organic Acids on the Nukol
- Application: The non-polar phase of this column results in the elution of fatty acid methyl esters (FAMEs) in essentially a boiling point order, useful when pattern recognition is used to detect subtle differences from sample to sample.
- USP Code: This column meets USP G1, G2, and G9 requirements.
- Polymer: Bonded; poly(dimethylsiloxane)
- Temperature Limits:
- -60 °C to 325/350 °C for 0.10 - 0.32 mm I.D.
- -60 °C to 300/320 °C for 0.53 mm I.D. (≤1.5 µm)
- -60 °C to 260/280 °C for 0.53 mm I.D. (>1.5 µm)
- Ordering Information for: Equity-1
Chromatograms:
37-Component FAME Mix on the Equity-1
Bacterial Acid Methyl Esters (BAMEs) on the Equity-1
- Application: This highly polar cyanosiloxane column is commonly used for separation of geometric (cis/trans) fatty acid methyl ester (FAME) isomers as a group. The phase is stabilized, providing a maximum temperature slightly higher than the SP-2560 column.
- USP Code: This column meets USP G48 requirements
- Polymer: Stabilized; poly(90% biscyanopropyl/10% cyanopropylphenyl siloxane)
- Temperature Limits: Subambient to 275 °C
- Ordering Information for: SP-2380
Chromatograms:
37-Component FAME Mix on the 30 m SP-2380
- Application: This column is specifically tuned to provide the necessary polarity for analyses of polyunsaturated fatty acid (PUFA) methyl esters with no overlap of the even carbon chain lengths.
- USP Code: This column meets USP G18 requirements
- Polymer: Bonded; poly(alkylene glycol)
- Temperature Limits: 50 °C to 220 °C
- Ordering Information for: SPB-PUFA
Chromatograms:
37-Component FAME Mix on the SPB-PUFA
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