FAME

FAME Solid Phase Extraction

Discovery Ag-Ion (silver-ion) SPE
When silver ions are loaded and immobilized on to an SCX phase as counter-ions, they have the ability to form polar complexes with unsaturated fatty acid double bonds under normal-phase conditions. Discovery Ag-Ion SPE was developed for the fractionation cis/trans isomers, and can also resolve FAMEs by degree of unsaturation in which retention strength increases with increasing number of double bonds. As a result, Discovery Ag-Ion SPE allows users to fractionate FAME samples prior to GC analysis thereby simplifying analytical chromatography and improving method accuracy.

Discovery Ag-Ion SPE phases are loaded with silver using a proprietary procedure to offer optimal resolution, performance, and capacity. Each lot is tested and quality controlled for cis/trans FAME resolution.
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  • Specifically developed for the fractionation of FAMEs based on degree of unsaturation, and the resolution of cis- and trans- isomers
  • Phase is loaded with silver using a proprietary procedure to offer optimal resolution, performance, and capacity
  • Each lot is tested and quality controlled for cis- and trans- FAME resolution
  • 750mg bed weight has a high capacity of 1mg FAMEs
 
Description Quantity Cat. No.
Discovery Ag-Ion SPE    
750 mg / 6 mL SPE Tube 30 54225-U
750 mg / 1 mL Rezorian™ cartridge 10 54226-U
   
Additional resources  
Title Publication ID.
Discovery® Ag-Ion SPE for FAME Fractionation and Cis/Trans Separation T406062
(603 Kb PDF)
Instruction sheet for using Discovery® Ag-Ion SPE T706002
(86 Kb PDF)

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