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Ascentis® Express 5 µm HPLC Columns
A new choice for improving performance of traditional HPLC systems!
Ascentis Express columns, based on 5 µm Fused-Core® particles, offer the benefits of faster and more efficient separations compared to columns based on 3 µm and 5 µm totally porous particles, without any backpressure concerns. Ascentis Express 5 µm columns are also extremely rugged, making them an ideal choice for bioanalytical LC-MS methods since they excel under the demands of high flow rate and high throughput.
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Available Phases: C18, F5, C8, Phenyl-Hexyl, ES-Cyano ,  HILIC, OH5
Special Offer: From May 1 through June 30, 2013, customers in Europe, US, and Canada can take advantage of a 50% discount on all Ascentis Express 5 µm HPLC columns. Use promotion code SFU at the time of placing an order. This offer does not include guard cartridges/holders, and is not valid in other regions.
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High Efficiency Particles
5 µm Ascentis Express Fused-Core particles provide double the number of plates per pressure relative to 5 µm totally porous particles, and four times the number of plates per pressure relative to 3 µm totally porous particles.
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Best Plates per Pressure
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| Comparison of Particle Characteristics |
| Particle Type |
Shell
Thickness (µm) |
BET Surface
Area (m2/g) |
Average Pore
Diameter (Å) |
Plates* |
Pressure*
(bar) |
Plates per
Pressure |
| 5 µm Ascentis Express Fused-Core |
0.6 |
90 |
90 |
28,300 |
78 |
363 |
| 3 µm totally porous |
N/A |
300 |
100 |
24,200 |
309 |
78 |
| 5 µm totally porous A |
N/A |
300 |
100 |
14,600 |
100 |
146 |
| 5 µm totally porous B |
N/A |
170 |
120 |
14,400 |
63 |
229 |
| 5 µm totally porous C |
N/A |
450 |
100 |
15,300 |
120 |
128 |
| * Plates and Pressure: reported for 1-chloro-4-nitrobenzene at the flow rate corresponding to the plate height minimum |
Kinetic Plots - Comparison of Particles
The 5 µm Fused-Core particle outperforms 3 µm and 5 µm totally porous particles under all conditions.
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| As a result, Ascentis Express 5 µm columns outperform popular 3 µm and 5 µm columns (based on totally porous particles), which are currently used in most HPLC systems. |
Better Performance - Faster & More Efficient Separation
The chromatograms below show a relative comparison of the separation obtained using an Ascentis Express 5 µm column vs that obtained using a popular column based on 5 µm totally porous particles; identical method conditions were used.
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Ascentis Express C18 5 µm, 15 cm x 4.6 mm I.D.
N=23,422 for last peak, 80 Bar, run time = 8 min
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| Conditions |
| Mobile Phase: |
50:50 water:acetonitrile |
| Flow Rate: |
1 mL/min |
| Detection: |
UV, 250 nm |
| Sample: |
Mix of polar and non-polar analytes |
| Analytes: |
1. Uracil
2. Acetophenone
3. Benzene
4. Toluene
5. Naphthalene |
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Popular C18 5 µm, 15 cm x 4.6 mm I.D.
N=13,633 for last peak, 72 Bar, run time = 16 min
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Trademarks:
Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc. |
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