|
Use
The classic reversed-phase column, Ascentis C18 is suitable for any method that specifies a C18-type column. Its high surface area gives Ascentis C18 strong hydrophobic retention and high loading capacity for preparative applications.
LC-MS Implications
Ascentis C18 is low-bleed for clean ESI and APCI traces. The high retentivity means that the mobile phase can contain high levels of organic modifier that are more readily desolvated.
Notes
Modern C18 columns have very similar selectivity, even for basic compounds, because silica quality and bonding techniques have improved to the point that silanol effects are minimal. Unless quality with your current column is the issue, the main reasons for evaluating different brands of C18 columns are for improved peak shape and for slight changes in selectivity.
* Under certain conditions, the Ascentis family can be operated in the extended pH range. For more details, see “Acid/Base Stability of Silica Based on C8, C18, and Amide HPLC Columns” (T406018 - 530Kb pdf)
|
