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Features
- Low-bleed for MS or UV gradient applications due to the use of trifunctional bonding reagent
- Outstanding phenyl selectivity due to high phase loading and short butyl spacer
- 100% aqueous-compatible for highly-polar compounds
Structure of Ascentis Phenyl
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| Properties |
| USP Code: |
L11 |
| Bonded Phase Description: |
Phenyl ring with short
butyl spacer |
| Endcapped: |
Yes |
| Particle composition: |
Type B silica gel |
| Particle Purity: |
<5 ppm metals |
| Particle Shape: |
Spherical |
| Particle Size: |
3, 5 and 10 µm |
| Pore Size: |
100 Å |
| Surface Area: |
450 m2/g |
| Carbon Load: |
19.5 % |
| pH Range (recommended): |
2 to 8 |
| Extended pH range*: |
1.5 to 10 |
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Key Applications
Small, water soluble molecules and peptides, π-acceptors, nitroaromatics, polar compounds, dipoles, heterocyclics, HILIC mode
Ordering Information for Ascentis Phenyl HPLC columns
Use
Phenyl phases are π-basic (electron donating) and are similar in overall retention to alkyl and EPG phases for easy column screening. The alternate selectivity of phenyl phases is often explained by the π-π interactions available through the phenyl ring. Compounds that appear to exhibit differential selectivity on the phenyl phase include:
- hydrophobic bases (TCAs, tetracyclines)
- moderate bases (anesthetics and narcotic analgesics)
- benzodiazepines
- some acidic compounds such as ACE inhibitors and quinoline antibiotics
- nucleosides (e.g. cytidine)
- nitro, azide and sulfonyl compounds
Notes
- Methanol can be a more selective mobile phase component than acetonitrile.
- Activate HILIC mode by using highly-organic (>90%) mobile phases.
* Under certain conditions, the Ascentis family can be operated in the extended pH range. For more details, see “Acid/Base Stability of Silica Based on C8, C18, and Amide HPLC Columns” (T406018 - 530Kb pdf)
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