HPLC

Chiral HPLC Columns from Astec & Supelco

Astec Products | Technical Resources 

Frequently Asked Questions - over 60 topics to assist you with chiral chromatography!

Astec CHIROBIOTIC™ Chiral HPLC Columns

CHIROBIOTIC phases, based on covalently bonded macrocyclic glycoproteins to high purity silica gel, offer stability while retaining essential components for chiral recognition. Useful for LC-MS chiral applications and when reversed-phase solvents (methanol/water, acetonitrile/water) are preferred over normal phase solvents.

Astec CYCLOBOND™ Chiral HPLC Columns

These bonded cyclodextrin stationary phases have the ability to form inclusion complexes, and allow for chemical separations by selectively including a wide variety of organic molecules from solution into the cyclodextrin cavity. CYCLOBOND I columns are ß-cyclodextrin bonded and CYCLOBOND II are γ-cyclodextrin bonded.

Astec Cellulose DMP Chiral HPLC Columns

Chiral stationary phase comprising spherical, high-purity porous silica coated with DMPC (3,5-dimethylphenylcarbamate)-derivatized cellulose, and packed in analytical to preparative size HPLC columns. It separates a wide range of chiral compounds under normal phase, polar organic, and SFC conditions. Performance is comparable to other DMPC-derivatized cellulose CSPs.

Astec Copper Ligand Exchange Chiral HPLC Columns

Using the copper ligand concept, this phase resolves hydroxy acids like lactic, malic, tartaric and mandelic acids. The CLC phase can also resolve amino acids, other amines, and bi-functional racemates like amino alcohols by the same mechanism. In theory, any analyte that can complete the coordination with the copper ion can be resolved.

Protein-based Chiral HPLC Columns

Chiral-AGP phase offers a1-acid glycoprotein as chiral selector, Chiral-CBH phase offers cellobiohydrolase as chiral selector, and Chiral-HSA phase offers human serum albumin as chiral selector - immobilized on porous 5 µm spherical silica particles, and typically used in reversed-phase mode.

Astec Polycyclic Amine Polymer Chiral HPLC Columns (P-CAP, P-CAP-DP)

These chiral phases offer a new bonding technology, primarily used in normal phase, for the separation of racemic compounds. Structural conformation and hydrogen bonding interactions are the driving mechanisms. Separations, including preparative, have been run in a variety of solvents including pure acetone, heptane/ethanol, dichloromethane/methanol and ethyl acetate.

 Kromasil Chiral HPLC Columns

Chiral stationary phases for HPLC, SFC and SMB based on the well-known Kromasil premium spherical silica.

LARIHC and FRULIC Chiral HPLC and HILIC Columns

Cyclofructans are cyclic oligosaccharides and the newest class of chiral stationary phases for HPLC, SFC, and HILIC. Invented by Prof. Daniel W. Armstrong and introduced by AZYP, the LARIHC and FRULIC derivatized cyclofructan-based HPLC columns are now available through Supelco/Sigma-Aldrich.