HPLC Columns

Discovery HPLC Columns

new phases
 
Biomolecules
Small Molecules (<2000 mw)

Discovery BIO GFC New!

Discovery BIO GFC phases perform size exclusion separations over a wide molecular weight range, from small biomolecules to virus particles. By eliminating non-specific adsorption, the unique hydrophilic surface treatment and ultra high-purity silica combine to allow reproducibly high recovery of active proteins. Discovery BIO GFC is available in 5 µm spherical particles with pore sizes from 100 to 2000 Å for high separation capacity. The narrow pore and particle size distributions ensure high column efficiency and reproducibility.


 
Discovery BIO PolyMA-SCX

Efficient, high-recovery polymer-based cation-exchange columns for protein and peptide separations.

 
Discovery BIO PolyMA-WAX

Efficient, high-recovery polymer-based anion-exchange columns for protein and peptide separations.

 
Discovery BIO Wide Pore C5

Similar retention but increased stability compared to C4 with excellent, no bleed properties for LC-MS. Used for proteins and hydrophobic peptides.
  Now in 0.18mm ID dimensions.

 
Discovery BIO Wide Pore C8

Intermediate hydrophobicity between a C4/C5 and C18 makes it a great starting point for peptide method development. Used for peptides and small proteins.

 
Discovery BIO Wide Pore C18

The most hydrophobic BIO Wide Pore phase. Excellent for synthetic and natural peptides and peptide mapping.
Now in 0.18mm ID dimensions.

Literature
   
   
Discovery BIO Wide Pore Brochure

Click to download the 36-page Discovery BIO Wide Pore brochure (9,866 KB pdf)

 
   
NEW! Discovery BIO PolyMA Brochure

Ion exchange columns for protein and peptide separations. (701KB pdf)

 
   
Technical Bulletin:
Peptide Separations on a Stable, Aqueous-Compatible C18 Column

Most C18 HPLC columns undergo phase collapse in 100% aqueous mobile phases with concomitant loss of retention. This causes a problem when trying to analyze very polar compounds. This Technical Bulletin describes how Discovery BIO Wide Pore C18 columns do not undergo phase collapse, and can be used in 100% aqueous conditions. The implications for polar peptides and peptides mapping is described.

(52KB pdf)
 
   
Getting Started with RP-HPLC for Peptides

Application Note 166 (69 KB pdf)

 
   
Increase Sensitivity and Decrease Sample Consumption Using HPLC Microbore and Capillary Column Dimensions

Application Note 167 (53 KB pdf)

 
   
Eliminate TFA and Improve Sensitivity of Peptide Analyses by LC-MS

Application Note 168 ( 58 KB pdf)

 
   
Effect of Stationary Phase on Selectivity of Reversed-Phase HPLC Separations of Polypeptides

Application Note 169 (75 KB pdf)

 
   
Discovery BIO GFC Separation Volume and Peak Capacity

Application Note 187 (512 KB pdf)

 

Short, Fast LC-MS Columns for Discovery Phases - C8, C18, cyano and HS F5 now available!
(more information)

Discovery C18 and C8 NEW sizes available!

Classic reversed-phase selectivity and retention with exceptional peak shape for all compounds. Excellent, no-bleed properties for LC-MS applications.

 
Discovery HS C18

Non-polar, reversed-phase column with excellent, no bleed LC-MS performance

 
Discovery RP-Amide C16

Polar-embedded, palmitamidopropyl reversed-phase column with unique retention and selectivity.

 
Discovery HS F5 NEW sizes available!

Pentafluorophenyl terminated reversed-phase column with unique retention and selectivity
(e.g. basic & halogenated compounds)

 
Discovery Cyano NEW sizes available!

Cyanopropyl reversed-phase column with lower hydrophobicity than C18 or C8 and unique selectivity.

 
Discovery HS PEG

Polyethylene glycol reversed-phase column with unique retention and selectivity
(e.g. phenolic compounds)

Discovery Zr

For HPLC methods at pH and temperature extremes.

 
Discovery Zr-PBD

Polybutadiene-coated zirconia particles for operation at pH 1-13 and up to 100°C (150°C in special hardware). Similar selectivity to C18-silica, but with pH and thermal stability.

 
Discovery Zr-PS

Polystyrene-coated zirconia particles for operation at pH 1-13 and up to 100°C (150°C in special hardware).

 
Discovery Zr-Carbon

Carbon-clad zirconia for separating isomers. Operates from pH 1-14 and up to 100°C (200°C in special hardware).

 
Discovery Zr-CarbonC18

C18-bonded carbon-clad zirconia combines shape selectivity with partitioning. Operates from pH 1-14 and up to 100°C (200°C in special hardware).

Literature
 

Discovery HPLC Columns for Small Molecule Separations

Rediscover HPLC method development with this 76-page brochure, a practical tutorial that describes how Discovery allows you to develop better HPLC methods and solve common HPLC problems. Contains method development case studies, guidelines to choosing a Discovery column based on analyte and separation problem. Also contains information on the new Discovery Zr products. (1,1251 KB pdf)

 
Discovery Zr Method Development

Bulletin 932 (48 KB pdf)