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| Discovery Zr-CarbonC18 HPLC columns |
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| Discovery Zr-CarbonC18 consists of spherical, porous carbon-clad zirconia particles covalently modified with octadecyl (C18) groups. It is intended for the reversed-phase separation of acidic and neutral compounds. It complements the selectivity offering of the other zirconia-, silica-, and polymer-based Discovery phases, and allows the use of the full range of mobile phase pH from pH 1 to 14. |
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| Particle: |
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Zirconia (ZrO2) |
| Bonded Phase: |
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Octadecyl(C18)-bonded Carbon |
| Particle Shape: |
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Spherical |
| Particle Size: |
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3 and 5µm |
| Pore Size: |
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300Å |
| Surface Area: |
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30m2/g |
| pH Range: |
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1 to 14 |
| Temperature Range: |
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up to 100°C in standard hardware (up to 200°C in custom all-steel hardware) |
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| Unique retention and selectivity for method development at pH and temperature extremes |
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Very different selectivity compared to C18-silica
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Resistant to phase hydrolysis
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pH stable from 1-14
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Thermally stable up to 200°C
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| RAPID LOW pH AND HIGH TEMPERATURE SEPARATIONS |
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| Discovery Zr-CarbonC18 has the partitioning mechanism of C18, but because it is bonded to a highly inert, carbonaceous support, it is immune to pH and temperature extremes. The examples of the acidic non-steroidal anti-inflammatory compounds and in the figures below run at pH 1.75 and 80°C on Discovery Zr-CarbonC18 demonstrates the extreme applicability of this phase. |
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| RAPID HIGH pH AND HIGH TEMPERATURE SEPARATIONS |
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| Increasing the temperature can greatly reduce the analysis time. The thermal stability of all Discovery Zr phases allows temperatures up to 100°C and higher with special hardware. The separation of ß-blockers on Discovery Zr-CarbonC18 at 80°C in less than 1 minute is shown in the figure below. |
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| PARTIONING AND SHAPE-SELECTIVE RETENTION MECHANISMS |
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| The separation of positional isomers of a proprietary sulfonamide drug is shown in the figure below. Here the parent compound is easily distinguished between its three corresponding positional isomers. |
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Description |
2.1mm ID |
4.0mm ID  |
| 1cm Supelguard Cartridges with Discovery Zr-CarbonC18 Packings |
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3µm Particle Size |
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65802-U |
65804-U |
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Kit  |
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65801-U |
65803-U |
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5µm Particle Size |
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65806-U |
65808-U |
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Kit |
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65805-U |
65807-U |
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For 4.0mm ID or 4.6mm ID analytical columns |
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Kit includes a holder and one replaceable cartridge. |
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 For additional information, click the pdf icons below. |
 Bulletin 932 - Discovery Zr: Method Development Guidlines (48 KB) |
 Care and Use Guide for Discovery Zr-CarbonC18 (15 KB) |
| TRADEMARKS |
| Discovery, and Supelguard are trademarks of Sigma-Aldrich Co. |
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