HPLC

Discovery Zr PS HPLC columns

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Ordering Information

Discovery Zr-PS HPLC columns
 
Discovery Zr-PS consists of spherical, porous zirconia particles modified with cross-linked polystyrene. It operates via reversed-phase and ion-exchange modes. Because it is weakly hydrophobic, it can be used with 100% aqueous mobile phases. It has unique selectivity especially for aromatic compounds. Discovery Zr-PS complements the selectivity offering of the other zirconia-, silica-, and polymer-based Discovery phases, and allows the use of the full range of mobile phase pH from pH 1 to 13.
 
Particle:   Zirconia (ZrO2)
Bonded Phase:   Cross-linked polystyrene
Particle Shape:   Spherical
Particle Size:   3 and 5µm
Pore Size:   300Å
Surface Area:   30m2/g
pH Range:   1 to 13
Temperature Range:   up to 100°C in standard hardware (up to 150°C in custom all-steel hardware)
 
Unique retention and selectivity for method development at pH and temperature extremes
 

Good for very hydrophobic compounds

Good for basic compounds and amines

Can be used in 100% aqueous mobile phases

pH stable from 1-13

Thermally stable up to 100°C


Here's the
Proof
 
FAST SEPARATIONS OF HYDROPHOBIC AMINES
 
The relatively polar surface of Discovery Zr-PS permits rapid analysis of hydrophobic compounds. Because of the stability of the underlying zirconia surface, analyses can be run at low and high pH, and temperatures up to 150°C. The figure below shows a rapid gradient of acetonitrile in pH 1.8 buffer which effectively resolved three aromatic, hydrophobic amine drugs.
 
 
ANALYZE QUATERNARY AMINES WITHOUT ION-PAIRING
 
Basic compounds, especially quaternary amines, often suffer from lack of hydrophobic retention on classical C18-silica phase. To remedy this, ion-pairing is employed. However, ion-pair agents have serious disadvantages. By running at high pH, the hydrophobicity of the amine is increased and ion-pair agents are not required. Discovery Zr-PS is stable at high pH. The figure below shows the separation of paraquat and diquat, two quaternary amines, on Discovery Zr-PS and C18-silica. Note that at pH 8, ion-pairing is not needed to have retention on the Discovery Zr-PS. Retention is due to both hydrophobicity and the presence of ion-exchange with the Lewis base mobile phase buffer.
 
figure M
 
DIFFICULT BASES HAVE EXCELLENT PEAK SHAPE
 
Another problem with basic compounds on silica is their tendency to tail because of silanol interactions. This can be avoided by running at high pH where the charge on the base is neutralized. However, silica is limited to below pH 8. The figure below shows a difficult pair of bases on Discovery Zr-PS at pH 12. The symmetrical peaks are testimony to the lack of undesirable secondary interactions.
 
figure N

Ordering Information
Phase ID (mm) Length (cm) Cat. No.
3µm Discovery Zr-PS Columns
  2.1 5 65737-U
  2.1 7.5 65738-U
  2.1 15 65739-U
  4.6 5 65740-U
  4.6 7.5 65741-U
  4.6 15 65742-U
5µm Discovery Zr-PS Columns
  2.1 5 65743-U
  2.1 15 65744-U
  4.6 5 65746-U
  4.6 15 65747-U
  4.6 25 65748-U
 

Description 2.1mm ID 4.0mm ID
1cm Supelguard Cartridges with Discovery Zr-PS Packings
  3µm Particle Size    
 
  2 Pack
65842-U 65844-U
 
  Kit triangle
65841-U 65843-U
  5µm Particle Size
 
  2 Pack
65846-U 65848-U
 
  Kit triangle
65845-U 65847-U
 
For 4.0mm ID or 4.6mm ID analytical columns
Kit includes a holder and one replaceable cartridge.
   

For additional information, click the pdf icons below.
view the pdf document Bulletin 932 - Discovery Zr: Method Development Guidelines (48 KB)
view the pdf document Care and Use Guide for Discovery Zr-PS (10 KB)

How to Contact Supelco
 
In USA:
Phone: 800-247-6628
  or 814-359-3441
fax: 800-325-5052
email: techservice@sial.com
 
Outside the USA: click here

TRADEMARKS
Discovery, and Supelguard are trademarks of Sigma-Aldrich Co.