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HPLC

HPLC Discovery HS C18

Discovery logo
Ordering Information

Discovery HS C18 HPLC Columns, 120Å;
3µm, 5µm and 10µm particle sizes available
 

Specifically developed for pharmaceutical analysis and purification

Ideal for LC/MS applications - No detectable bleed (as independently tested)

Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility and long column life

Scalable from analytical to prep


Here's the
Proof
 
NO DETECTABLE LC/MS BLEED
 

Discovery HS C18

No TIC Baseline rise.
Essentially no m/z peaks generated from column ("x" indicates peaks also found in blank run)

 

Competitor C18

TIC Baseline rise can interfere with sensitivity, and quantitation measurements. Bleed m/z peaks could coincidently interfere with "real" peaks.

 

Column: Discovery HS C18 and Conventional C18, 5cm x 4.6mm, 3µm; Mobile Phase: 15 min. gradient from 0-100% MeOH in 0.1% TFA; Flow Rate: 1mL/min; Temp: ambient, esi+ ion.

g001413_14

(work conducted by Dr. Shane Needham, Alturas Analytics, Moscow, ID)


LOT TO LOT REPRODUCIBILITY OF DISCOVERY HS C18
 

Column: Discovery HS C18, 15cm x 4.6mm, 5µm;
Mobile Phase:
80% 10mM ammonium acetate, pH 6.8 : 20% MeOH;
Flow Rate:
1mL/min;
Temp:
35°C;
Detection:
254nm;
Injection:
1µL

1. Uracil (8µg/mL)
2. Procainamide (30µg/mL)
3. Sorbic acid (4µg/mL)
4. Pyridine (100µg/mL)
5. Caffeine (40µg/mL)
6. Phenol (240µg/mL)

 

g001597_9

GREATER HYDROPHOBIC RETENTION COMPARED TO OTHER POPULAR C18 PHASES
 

Columns: 7.5cm x 4.6mm, 3µm/3.5µm; Mobile Phase: 35:65 - H2O:ACN; Flow Rate: 2mL/min; Detection: 254nm

g001500


Ordering Information
3µm Discovery HS C18 HPLC Columns
ID(mm) Length(cm) Cat. No.
2.1 5 569253-U
2.1 7.5 569254-U
2.1 10 CUSTOM
2.1 15 569255-U
4.6 5 569250-U
4.6 7.5 569251-U
4.6 10 CUSTOM
4.6 15 569252-U
5µm Discovery HS C18 HPLC Columns
ID(mm) Length(cm) Cat. No.
2.1 5 568500-U
2.1 10 568501-U
2.1 15 568502-U
2.1 25 568503-U
4.0 5 568510-U
4.0 10 568511-U
4.0 15 568512-U
4.0 25 568513-U
4.6 5 568520-U
4.6 10 568521-U
4.6 15 568522-U
4.6 25 568523-U
10.0 5 568530-U
10.0 10 568531-U
10.0 15 568532-U
10.0 25 568533-U
21.2 5 568540-U
21.2 10 568541-U
21.2 15 568542-U
21.2 25 568543-U
10µm Discovery HS C18 HPLC Columns
ID(mm) Length(cm) Cat. No.
10.0 5 568630-U
10.0 10 568631-U
10.0 15 568632-U
10.0 25 568633-U
21.2 5 568640-U
21.2 10 568641-U
21.2 15 568642-U
21.2 25 568643-U
2cm Supelguard Cartridges with Discovery HS C18 Packings
  Description 2.1mm ID 4.0mm ID
  3µm Particle Size    
 
  2 Pack
569276-U 569274-U
 
  Kit triangle
569277-U 569275-U
  5µm Particle Size    
 
  2 Pack
568570-U 568572-U
 
  Kit triangle
568571-U 568573-U
 
For 4.0mm ID or 4.6mm ID analytical columns
triangle Kit includes cartridge, stand-alone holder, tubing, 2 nuts and ferrules.
   
1cm Supelguard Cartridges with Discovery HS C18 Packings
  Description 10mm ID
  5µm Particle Size 568574-U
  10µm Particle Size 568674-U

To download a printable copy of this information in PDF format, click here.

How to Contact Supelco
 
In USA:
Phone: 800-247-6628
  or 814-359-3441
fax: 800-325-5052
email: techservice@sial.com
 
Outside the USA: click here

TRADEMARKS
Discovery, Supelcosil, and Supelguard are trademarks of Sigma-Aldrich Co.
Waters Spherisorb and Waters Symmetry are registered trademarks of Waters Corp.
Phenomenex is a registered trademark of Phenomenex, Inc.
Luna is a trademark of Phenomenex, Inc.
Hypersil is a registered trademark of Hypersil Limited.