|
|
|
|
Discovery HS C18 HPLC Columns, 120Å;
3µm, 5µm and 10µm particle sizes available |
| |
|
•
|
Specifically developed for pharmaceutical analysis and purification
|
|
•
|
Ideal for LC/MS applications - No detectable bleed (as independently tested)
|
|
•
|
Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility and long column life
|
|
•
|
Scalable from analytical to prep
|
|
|
|
|
| |
| NO DETECTABLE LC/MS BLEED |
| |
|
Discovery HS C18
|
|
No TIC Baseline rise.
Essentially no m/z peaks generated from column ("x" indicates peaks also found in blank run)
|
|
|
|
Competitor C18
|
|
TIC Baseline rise can interfere with sensitivity, and quantitation measurements. Bleed m/z peaks could coincidently interfere with "real" peaks.
|
|
|
| |
|
Column: Discovery HS C18 and Conventional C18, 5cm x 4.6mm, 3µm; Mobile Phase: 15 min. gradient from 0-100% MeOH in 0.1% TFA; Flow Rate: 1mL/min; Temp: ambient, esi+ ion.
|
|
 |
|
(work conducted by Dr. Shane Needham, Alturas Analytics, Moscow, ID)
|
|
|
| LOT TO LOT REPRODUCIBILITY OF DISCOVERY HS C18 |
| |
|
Column: Discovery HS C18, 15cm x 4.6mm, 5µm;
Mobile Phase: 80% 10mM ammonium acetate, pH 6.8 : 20% MeOH;
Flow Rate: 1mL/min;
Temp: 35°C;
Detection: 254nm;
Injection: 1µL
1. Uracil (8µg/mL)
2. Procainamide (30µg/mL)
3. Sorbic acid (4µg/mL)
4. Pyridine (100µg/mL)
5. Caffeine (40µg/mL)
6. Phenol (240µg/mL)
|
|
 |
|
|
| GREATER HYDROPHOBIC RETENTION COMPARED TO OTHER POPULAR C18 PHASES |
| |
|
Columns: 7.5cm x 4.6mm, 3µm/3.5µm; Mobile Phase: 35:65 - H2O:ACN; Flow Rate: 2mL/min; Detection: 254nm
|
|
|
|
| Ordering Information |
| 3µm Discovery HS C18 HPLC Columns |
| ID(mm) |
Length(cm) |
Cat. No. |
| 2.1 |
5 |
569253-U |
| 2.1 |
7.5 |
569254-U |
| 2.1 |
10 |
CUSTOM |
| 2.1 |
15 |
569255-U |
| 4.6 |
5 |
569250-U |
| 4.6 |
7.5 |
569251-U |
| 4.6 |
10 |
CUSTOM |
| 4.6 |
15 |
569252-U |
|
| 5µm Discovery HS C18 HPLC Columns |
| ID(mm) |
Length(cm) |
Cat. No. |
| 2.1 |
5 |
568500-U |
| 2.1 |
10 |
568501-U |
| 2.1 |
15 |
568502-U |
| 2.1 |
25 |
568503-U |
| 4.0 |
5 |
568510-U |
| 4.0 |
10 |
568511-U |
| 4.0 |
15 |
568512-U |
| 4.0 |
25 |
568513-U |
| 4.6 |
5 |
568520-U |
| 4.6 |
10 |
568521-U |
| 4.6 |
15 |
568522-U |
| 4.6 |
25 |
568523-U |
| 10.0 |
5 |
568530-U |
| 10.0 |
10 |
568531-U |
| 10.0 |
15 |
568532-U |
| 10.0 |
25 |
568533-U |
| 21.2 |
5 |
568540-U |
| 21.2 |
10 |
568541-U |
| 21.2 |
15 |
568542-U |
| 21.2 |
25 |
568543-U |
|
| 10µm Discovery HS C18 HPLC Columns |
| ID(mm) |
Length(cm) |
Cat. No. |
| 10.0 |
5 |
568630-U |
| 10.0 |
10 |
568631-U |
| 10.0 |
15 |
568632-U |
| 10.0 |
25 |
568633-U |
| 21.2 |
5 |
568640-U |
| 21.2 |
10 |
568641-U |
| 21.2 |
15 |
568642-U |
| 21.2 |
25 |
568643-U |
|
| 2cm Supelguard Cartridges with Discovery HS C18 Packings |
| |
Description |
2.1mm ID |
4.0mm ID  |
| |
3µm Particle Size |
|
|
| |
|
569276-U |
569274-U |
| |
| |
Kit  |
|
569277-U |
569275-U |
| |
5µm Particle Size |
|
|
| |
|
568570-U |
568572-U |
| |
| |
Kit |
|
568571-U |
568573-U |
| |
|
For 4.0mm ID or 4.6mm ID analytical columns |
|
Kit includes cartridge, stand-alone holder, tubing, 2 nuts and ferrules. |
|
|
|
|
| 1cm Supelguard Cartridges with Discovery HS C18 Packings |
| |
Description |
10mm ID |
| |
5µm Particle Size |
568574-U |
| |
10µm Particle Size |
568674-U |
|
|
 To download a printable copy of this information in PDF format, click here. |
| TRADEMARKS |
| Discovery, Supelcosil, and Supelguard are trademarks of Sigma-Aldrich Co. |
| Waters Spherisorb and Waters Symmetry are registered trademarks of Waters Corp. |
| Phenomenex is a registered trademark of Phenomenex, Inc. |
| Luna is a trademark of Phenomenex, Inc. |
| Hypersil is a registered trademark of Hypersil Limited. |
|
|
