Analytical / Chromatography > HPLC > Columns > Discovery^® > HS C18

HPLC

HS C18

Discovery HS C18 HPLC Columns, 120Å;
3µm, 5µm and 10µm particle sizes available

•	Specifically developed for pharmaceutical analysis and purification
•	Ideal for LC/MS applications - No detectable bleed (as independently tested)
•	Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility and long column life
•	Scalable from analytical to prep

Here's the

Proof

NO DETECTABLE LC/MS BLEED

Discovery HS C18

No TIC Baseline rise.
Essentially no m/z peaks generated from column ("x" indicates peaks also found in blank run)

Competitor C18

TIC Baseline rise can interfere with sensitivity, and quantitation measurements. Bleed m/z peaks could coincidently interfere with "real" peaks.

Column: Discovery HS C18 and Conventional C18, 5cm x 4.6mm, 3µm; Mobile Phase: 15 min. gradient from 0-100% MeOH in 0.1% TFA; Flow Rate: 1mL/min; Temp: ambient, esi+ ion.

(work conducted by Dr. Shane Needham, Alturas Analytics, Moscow, ID)

LOT TO LOT REPRODUCIBILITY OF DISCOVERY HS C18

Column: Discovery HS C18, 15cm x 4.6mm, 5µm;
Mobile Phase: 80% 10mM ammonium acetate, pH 6.8 : 20% MeOH;
Flow Rate: 1mL/min;
Temp: 35°C;
Detection: 254nm;
Injection: 1µL

1. Uracil (8µg/mL)
2. Procainamide (30µg/mL)
3. Sorbic acid (4µg/mL)
4. Pyridine (100µg/mL)
5. Caffeine (40µg/mL)
6. Phenol (240µg/mL)

GREATER HYDROPHOBIC RETENTION COMPARED TO OTHER POPULAR C18 PHASES

Columns: 7.5cm x 4.6mm, 3µm/3.5µm; Mobile Phase: 35:65 - H₂O:ACN; Flow Rate: 2mL/min; Detection: 254nm

g001500

Ordering Information

3µm Discovery HS C18 HPLC Columns

ID(mm)	Length(cm)	Cat. No.
2.1	5	569253-U
2.1	7.5	569254-U
2.1	10	CUSTOM
2.1	15	569255-U
4.6	5	569250-U
4.6	7.5	569251-U
4.6	10	CUSTOM
4.6	15	569252-U

5µm Discovery HS C18 HPLC Columns

ID(mm)	Length(cm)	Cat. No.
2.1	5	568500-U
2.1	10	568501-U
2.1	15	568502-U
2.1	25	568503-U
4.0	5	568510-U
4.0	10	568511-U
4.0	15	568512-U
4.0	25	568513-U
4.6	5	568520-U
4.6	10	568521-U
4.6	15	568522-U
4.6	25	568523-U
10.0	5	568530-U
10.0	10	568531-U
10.0	15	568532-U
10.0	25	568533-U
21.2	5	568540-U
21.2	10	568541-U
21.2	15	568542-U
21.2	25	568543-U

10µm Discovery HS C18 HPLC Columns

ID(mm)	Length(cm)	Cat. No.
10.0	5	568630-U
10.0	10	568631-U
10.0	15	568632-U
10.0	25	568633-U
21.2	5	568640-U
21.2	10	568641-U
21.2	15	568642-U
21.2	25	568643-U

2cm Supelguard Cartridges with Discovery HS C18 Packings

Description

2.1mm ID

4.0mm ID

3µm Particle Size

2 Pack

569276-U

569274-U

Kit

569277-U

569275-U

5µm Particle Size

2 Pack

568570-U

568572-U

Kit

568571-U

568573-U

	For 4.0mm ID or 4.6mm ID analytical columns
	Kit includes cartridge, stand-alone holder, tubing, 2 nuts and ferrules.

1cm Supelguard Cartridges with Discovery HS C18 Packings

	Description	10mm ID
	5µm Particle Size	568574-U
	10µm Particle Size	568674-U

To download a printable copy of this information in PDF format, click here.

How to Contact Supelco

In USA:

Phone: 800-247-6628

or 814-359-3441

fax: 800-325-5052

email: techservice@sial.com

Outside the USA: click here

TRADEMARKS

Discovery, Supelcosil, and Supelguard are trademarks of Sigma-Aldrich Co.

Waters Spherisorb and Waters Symmetry are registered trademarks of Waters Corp.

Phenomenex is a registered trademark of Phenomenex, Inc.

Luna is a trademark of Phenomenex, Inc.

Hypersil is a registered trademark of Hypersil Limited.

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