HPLC

Discovery HS PEG HPLC Columns

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Ordering Information

Discovery HS PEG HPLC Columns 120Å;
3µm, 5µm and 10µm particle sizes available

A unique polyethylene glycol reversed phase column specifically developed for pharmaceutical

 

analysis and purification

Unique selectivity and faster separations of polar compounds compared to C18 phases

Outstanding performance for phenolic compounds offers unique retention and selectivity and

 

faster analysis compared to C18

Scalable from analytical to prep

Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility and long column life


Here's the
Proof
 
UNIQUE SELECTIVITY AND FASTER SEPARATION OF POLAR COMPOUNDS
 

Discovery HS PEG delivers unique retention, selectivity and faster separations of polar compounds when compared to C18 phases.

 

Columns: Conventional C18 phase and Discovery HS PEG, 15cm x 4.6mm, 5µm; Mobile Phase: 85% 10mM ammonium acetate, pH 6.8 : 15% MeCN; Flow Rate: 1.0mL/min; Temp: 20°C; Detection: UV/Photodiode Array; Injection: 10µL (50µg/mL for each analyte)

 

Conventional C18 Phase

 

1. Uracil
2. Benzamide
3. Acetanilide
4. Phenol
5. N-Methylaniline
6. Nitrobenzene

g001530
 

Discovery HS PEG

 

1. Uracil
2. Benzamide
3. Acetanilide
4. Phenol
5. N-Methylaniline
6. Nitrobenzene

g001531

OUTSTANDING RESOLUTION OF PHENOLIC
COMPOUNDS ON DISCOVERY HS PEG
 

Columns: Conventional C18 phase and Discovery HS PEG, 15cm x 4.6mm, 5µm; Mobile Phase: 85% 10mM ammonium acetate, pH 6.8 : 15% MeCN; Flow Rate: 1.0mL/min; Temp: 20°C; Detection: UV/Photodiode Array; Injection: 10µL (50µg/mL for each analyte)

1. Uracil
2. Phloroglucinicol
3. Pyrogallol
4. Resorcinol
5. Benzamide
6. Catechol
7. Phenol
8. Nitrobenzene
9. Phenetole

g001532_3

Especially note the improved retention of Phloroglucinicol (Peak 2) and Phenetole (Peak 9) on the Discovery HS PEG phase.


LOT TO LOT REPRODUCIBILITY OF DISCOVERY HS PEG
 

Column: Discovery HS PEG, 15cm x 4.6mm, 5µm; Mobile Phase: 45% 10mm potassium phosphate, pH 7 : 55% MeOH; Flow Rate: 1mL/min; Temp: 35°C; Detection: 254nm; Injection: 5µL

1. Uracil (8µg/mL)
2. Pyridine (20µg/mL)
3. Phenol (300µg/mL)

g001517_9

Ordering Information
3µm Discovery HS PEG HPLC Columns
ID(mm) Length(cm) Cat. No.
2.1 5 567400-U
2.1 10 567402-U
2.1 15 567403-U
4.0 5 567430-U
4.0 10 567431-U
4.0 15 567432-U
4.6 5 567404-U
4.6 10 567406-U
4.6 15 567407-U
5µm Discovery HS PEG HPLC Columns
ID(mm) Length(cm) Cat. No.
2.1 5 567408-U
2.1 10 567410-U
2.1 15 567411-U
2.1 25 567412-U
4.0 5 567433-U
4.0 10 567434-U
4.0 15 567435-U
4.0 25 567436-U
4.6 5 567413-U
4.6 10 567415-U
4.6 15 567416-U
4.6 25 567417-U
10.0 5 567418-U
10.0 10 567437-U
10.0 15 567419-U
10.0 25 567420-U
21.2 5 567421-U
21.2 10 567439-U
21.2 15 567422-U
21.2 25 567423-U
10µm Discovery HS PEG HPLC Columns
ID(mm) Length(cm) Cat. No.
10.0 5 567424-U
10.0 10 567438-U
10.0 15 567425-U
10.0 25 567426-U
21.2 5 567427-U
21.2 10 567440-U
21.2 15 567428-U
21.2 25 567429-U
2cm Supelguard Cartridges with Discovery HS PEG Packings
  Description 2.1mm ID 4.0mm ID
  3µm Particle Size
 
  2 Pack
567470-U 567472-U
 
  Kit triangle
567471-U 567473-U
  5µm Particle Size
 
  2 Pack
567474-U 567476-U
 
  Kit triangle
567475-U 567477-U
 
For 4.0mm ID or 4.6mm ID analytical columns
triangle Kit includes cartridge, stand-alone holder, tubing, 2 nuts and ferrules.
   
1cm Supelguard Cartridges with Discovery HS PEG Packings
  Description 10mm ID
  5µm Particle Size 567478-U
  10µm Particle Size 567480-U

pdf icon To download a printable copy of this information in PDF format, click here.

How to Contact Supelco
 
In USA:
Phone: 800-247-6628
  or 814-359-3441
fax: 800-325-5052
email: techservice@sial.com
 
Outside the USA: click here

TRADEMARKS
Discovery and Supelguard are trademarks of Sigma-Aldrich Co.