Analytical / Chromatography > HPLC > Columns > Discovery^® > HS PEG

HPLC

HS PEG

Discovery HS PEG HPLC Columns 120Å;
3µm, 5µm and 10µm particle sizes available

•	A unique polyethylene glycol reversed phase column specifically developed for pharmaceutical
	analysis and purification
•	Unique selectivity and faster separations of polar compounds compared to C18 phases
•	Outstanding performance for phenolic compounds offers unique retention and selectivity and
	faster analysis compared to C18
•	Scalable from analytical to prep
•	Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility and long column life

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UNIQUE SELECTIVITY AND FASTER SEPARATION OF POLAR COMPOUNDS

Discovery HS PEG delivers unique retention, selectivity and faster separations of polar compounds when compared to C18 phases.

Conventional C18 Phase

1. Uracil
2. Benzamide
3. Acetanilide
4. Phenol
5. N-Methylaniline
6. Nitrobenzene

Discovery HS PEG

1. Uracil
2. Benzamide
3. Acetanilide
4. Phenol
5. N-Methylaniline
6. Nitrobenzene

OUTSTANDING RESOLUTION OF PHENOLIC
COMPOUNDS ON DISCOVERY HS PEG

Columns: Conventional C18 phase and Discovery HS PEG, 15cm x 4.6mm, 5µm; Mobile Phase: 85% 10mM ammonium acetate, pH 6.8 : 15% MeCN; Flow Rate: 1.0mL/min; Temp: 20°C; Detection: UV/Photodiode Array; Injection: 10µL (50µg/mL for each analyte)

1. Uracil
2. Phloroglucinicol
3. Pyrogallol
4. Resorcinol
5. Benzamide
6. Catechol
7. Phenol
8. Nitrobenzene
9. Phenetole

Especially note the improved retention of Phloroglucinicol (Peak 2) and Phenetole (Peak 9) on the Discovery HS PEG phase.

LOT TO LOT REPRODUCIBILITY OF DISCOVERY HS PEG

Column: Discovery HS PEG, 15cm x 4.6mm, 5µm; Mobile Phase: 45% 10mm potassium phosphate, pH 7 : 55% MeOH; Flow Rate: 1mL/min; Temp: 35°C; Detection: 254nm; Injection: 5µL

1. Uracil (8µg/mL)
2. Pyridine (20µg/mL)
3. Phenol (300µg/mL)

Ordering Information

3µm Discovery HS PEG HPLC Columns

ID(mm)	Length(cm)	Cat. No.
2.1	5	567400-U
2.1	10	567402-U
2.1	15	567403-U
4.0	5	567430-U
4.0	10	567431-U
4.0	15	567432-U
4.6	5	567404-U
4.6	10	567406-U
4.6	15	567407-U

5µm Discovery HS PEG HPLC Columns

ID(mm)	Length(cm)	Cat. No.
2.1	5	567408-U
2.1	10	567410-U
2.1	15	567411-U
2.1	25	567412-U
4.0	5	567433-U
4.0	10	567434-U
4.0	15	567435-U
4.0	25	567436-U
4.6	5	567413-U
4.6	10	567415-U
4.6	15	567416-U
4.6	25	567417-U
10.0	5	567418-U
10.0	10	567437-U
10.0	15	567419-U
10.0	25	567420-U
21.2	5	567421-U
21.2	10	567439-U
21.2	15	567422-U
21.2	25	567423-U

10µm Discovery HS PEG HPLC Columns

ID(mm)	Length(cm)	Cat. No.
10.0	5	567424-U
10.0	10	567438-U
10.0	15	567425-U
10.0	25	567426-U
21.2	5	567427-U
21.2	10	567440-U
21.2	15	567428-U
21.2	25	567429-U

2cm Supelguard Cartridges with Discovery HS PEG Packings

Description

2.1mm ID

4.0mm ID

3µm Particle Size

2 Pack

567470-U

567472-U

Kit

567471-U

567473-U

5µm Particle Size

2 Pack

567474-U

567476-U

Kit

567475-U

567477-U

	For 4.0mm ID or 4.6mm ID analytical columns
	Kit includes cartridge, stand-alone holder, tubing, 2 nuts and ferrules.

1cm Supelguard Cartridges with Discovery HS PEG Packings

	Description	10mm ID
	5µm Particle Size	567478-U
	10µm Particle Size	567480-U

To download a printable copy of this information in PDF format, click here.

How to Contact Supelco

In USA:

Phone: 800-247-6628

or 814-359-3441

fax: 800-325-5052

email: techservice@sial.com

Outside the USA: click here

TRADEMARKS

Discovery and Supelguard are trademarks of Sigma-Aldrich Co.

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