Solvents

HPLC CHROMASOLV® - Solvents

LC-MS CHROMASOLV® - Solvents for LC-MS Analysis

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LC-NMR CHROMASOLV® - Solvents for LC-NMR Analysis

IMPROVE STABILITY OF LC-MS BASELINES BY USING HIGH-QUALITY SOLVENTS

Using LC-MS CHROMASOLV® eliminates impurities and the need to lot-select your solvents.

LC-MS is fast becoming the dominant analytical tool for researchers in a wide range of industries and application areas. LC-MS combines the advantages of a chromatographic separation with mass detection by MS. Low detection limits and qualified analysis of molecular structures brought this application a new and growing popularity especially in the field of biotechnology and within the R&D departments of the pharmaceutical industry.

Besides the column and instrument, the solvent can seriously affect the performance of an LC-MS system. Impurities arising from the solvent can mask low levels of target ions, or give false peaks, resulting in faulty interpretation of spectra. Sigma-Aldrich's LC-MS CHROMASOLV solvents is manufactured specifically to meet the needs of modern LC-MS instruments because they are designed to have low contents (max. 100 ppb) of alkaline impurities, such as calcium, magnesium, potassium and sodium, which can form clusters with the analyte, especially when using electro spray ionization. All LC-MS CHROMASOLV solvents undergo a battery of specific UV-spectroscopic quality control tests to guarantee the CHROMASOLV specifications and to ensure the reproducibility. They are also checked for LC-MS use by a special test and for particles, which could clog up Nano-LC inlet filters.

For more details see our latest research: pdf 370 KB.

Specifications/Product Range

LC-MS blends
LC-MS allows the detection and quantification of many analytes. The minimisation of artefacts requires very well specified solvents spiked with ultra pure salts and acids. These additives are used to improve the chromatographic peak shape and to optimise ionisation in the MS interface. The most commonly used solvents in LC-MS techniques are acetonitrile, methanol and water. Additives can include trifluoracetic acid, formic acid, acetic acid and ammonium acetate.

For more details see our latest Labinfo: pdf 104 KB

Although an efficient separation method is available by HPLC, no structure determination is possible when detecting separated compounds by UV-detector. Thus NMR spectroscopy is a better universial detection method. A combination of both methods was evident and LC-NMR was born.

Solvents that meet the high requirements of HPLC and proton NMR are needed. But the high throughput of solvents in HPLC does not allow the usage of expensive deuterated solvents as usually required for NMR. HPLC Acetonitrile is often contaminated with impurities containing protons such as propionitrile which is no problem in UV but NMR detection. Therefore a dramatic decrease of proton containing contaminants for LC-NMR solvents is essential.

Acetonitrile LC-NMR CHROMASOLV® (34955) from is the solvent of choice. It was developed to obtain the superior specifications required for LC-NMR. There are no further signals in ¹H-NMR that exceed the size of methyl signals (more than 5% at 3.78 ppm NMR of 0.0006% trimethoxybenzene) at typical LC-NMR conditions.

For additional information, please contact your local Sigma-Aldrich office.

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