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Analytical / Chromatography > HPLC > TSKGEL HPLC Columns
HPLC

TSKGEL HPLC Columns
TSK-GEL® columns are available for gel filtration, gel permeation, ion exchange, reversed phase, hydrophobic interaction, and affinity chromatography.

TSK-GEL and TOYOPEARL® are widely popular columns and packing materials for the HPLC separation of proteins, peptides and other biomolecules. TSK-GEL columns are packed with either silica or methacrylate particles with pore sizes to accommodate a wide range of analyte molecular weights. Both particles feature surface chemistry designed to minimize sample adsorption. All popular biomolecule separation modes are covered by TSK-GEL columns, including size exclusion (gel filtration and gel permeation), ion exchange, affinity, reversed-phase, HIC (hydrophobic interaction) and HILIC (hydrophilic interaction) chromatography.

View the complete listing of all TSKGEL columns and guard columns, and related products plus ToyoScreen® packed columns.
Size Exclusion Anion-Exchange Columns
- TSK-GEL SW Series Gel Filtration Columns (Silica-Based) Cation-Exchange Columns
- TSK-GEL PW Series Gel Filtration Columns (Polymer-Based) Reversed-Phase Columns
- TSK-GEL HHR Series Gel Permeation Columns Affinity Columns
- TSK-GEL HXL Series Gel Permeation Columns Hydrophobic Interaction Columns
Anion-Exchange Columns back to top
Description Cat. No.
Column  
DEAE-5PW particle size 10 µm length × I.D. 7.5 cm × 7.5 mm 807164
DEAE-2SW particle size 5 µm length × I.D. 25 cm × 4.6 mm 807168
DEAE-NPR particle size 2.5 µm length × I.D. 3.5 cm × 4.6 mm 813075
Guard Column Kit1  
DEAE-NPW particle size 5 µm length × I.D. 0.5 cm × 4.6 mm 817088
DEAE-5PW particle size 20 µm length × I.D. 1 cm × 6.0 mm 807210
DEAE-SW particle size 10 µm length × I.D. 1 cm × 6.0 mm 807648
Cation-Exchange Columns back to top
Description Cat. No.
Column  
CM-5PW particle size 10 µm length × I.D. 7.5 cm × 7.5 mm 813068
SP-NPR particle size 2.5 µm length × I.D. 3.5 cm × 4.6 mm 813076
SP-5PW particle size 10 µm length × I.D. 7.5 cm × 7.5 mm 807161
CM-2SW particle size 5 µm length × I.D. 25 cm × 4.6 mm 807167
CM-3SW particle size 10 µm length × I.D. 7.5 cm × 7.5 mm 807162
Guard Column Kit1  
CM-5PW particle size 20 µm length × I.D. 1 cm × 6 mm 813069
SP-5PW particle size 20 µm length × I.D. 1 cm × 6 mm 807211
CM-SW particle size 10 µm length × I.D. 1 cm × 6 mm 807650
1 Guard column kit contains one cartridge, a stand-alone holder, 5 mL packing, 5 cm of 1/16 in. tubing, and 2 nuts and ferrules.  
   
Additional resources  
Title
Ion Exchange Columns (45 Kb PDF) id. T494077
TSK-GEL NPR Columns (87 Kb PDF) id. T109862
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Size Exclusion  
TSK-GEL SW Series Gel Filtration Columns (Silica-Based) back to top
TSK-GEL SW and TSK-GEL SWXL columns contain silica-based, hydrophilic bonded phase packings that minimize interaction with proteins. A 30 cm TSK-GEL SWXL column and a 60 cm TSK-GEL SW column provide similar resolution, but the SWXL column requires half the analysis time. Sample capacity increases in proportion with column length.

Because TSK-GEL SWXL and TSK-GEL SW columns are silica based, they must be operated within the recommended pH range of 2.5 - 7.5. Detailed operating conditions are described in the information accompanying the columns. We recommend protecting these columns with the appropriate SWXL or SW guard column.
Glutathione S-Transferase
Column:   TSK-GEL G3000SWXL, 30 cm x 7.8 mm I.D., 5 µm particles
Cat. No.:   808541
Mobile Phase:   0.3M NaCl/0.05M NaH2PO4, pH 7.0
Flow Rate:   1 mL/min
Temp.:   25 °C
Det.:   UV, 220 nm
Injection:   100 µL crude glutathione S-transferase preparation from guinea pig liver (0.7mg)
  713-0939
Description Cat. No.
G2000SWXL particle size 5 µm length × I.D. 30 cm × 7.8 mm 808540
G3000SWXL particle size 5 µm length × I.D. 30 cm × 7.8 mm 808541
G4000SWXL particle size 8 µm length × I.D. 30 cm × 7.8 mm 808542
Super SW2000 particle size 4 µm length × I.D. 30 cm × 4.6 mm 818674
Super SW3000 particle size 4 µm length × I.D. 30 cm × 4.6 mm 818675
G2000SW particle size 10 µm length × I.D. 30 cm × 7.5 mm 805788
G2000SW particle size 10 µm length × I.D. 60 cm × 7.5 mm 805102
G3000SW particle size 10 µm length × I.D. 30 cm × 7.5 mm 805789
G3000SW particle size 10 µm length × I.D. 60 cm × 7.5 mm 805103
G3000SW particle size 13 µm length × I.D. 60 cm × 21.5 mm 805147
G4000SW particle size 13 µm length × I.D. 30 cm × 7.5 mm 805790
G4000SW particle size 13 µm length × I.D. 60 cm × 7.5 mm 805104
TSK-GEL
Column		 Particle Size (µm) Pore Size (Å) Sample MW
(globular proteins)
Super SW2000 4 125 5-150 x 103
G2000SWXL 5 125 5-150 x 103
G2000SW 10 125 5-100 x 103
Super SW3000 4 250 10-500 x 103
G3000SWXL 5 250 10-500 x 103
G3000SW 10 250 10-500 x 103
G4000SWXL 8 450 20-10,000 x 103
G4000SW 13 450 20-10,000 x 103
Mobile Phase: 0.03 M NaCl in 0.1 M phosphate buffer, pH 7.0
Additional resources  
Title
TSK-GEL SW and SWXL Columns (56 Kb PDF) id. T494076
 
TSK-GEL PW Series Gel Filtration Columns (Polymer-Based) back to top
TSK-GEL PW and TSK-GEL PWXL columns are used in high performance gel filtration separations of water-soluble polymers and oligosaccharides. The hydrophilic polymer matrix has excellent chemical and mechanical stability. Although commonly used with aqueous solvents, the polymer is compatible with up to 50% organic solvent.

We recommend using a TSK-GEL PW guard columns with G2500PW - G6000PW columns. Use a TSK-GEL PWXL guard column with any PWXL column.

Bulk packing can be ordered to repack PW columns and guard columns.
Description Cat. No.
G2500PWXL particle size 6 µm length × I.D. 30 cm × 7.8 mm 808020
G3000PWXL particle size 6 µm length × I.D. 30 cm × 7.8 mm 808021
G4000PWXL particle size 10 µm length × I.D. 30 cm × 7.8 mm 808022
G5000PWXL particle size 10 µm length × I.D. 30 cm × 7.8 mm 808023
G6000PWXL particle size 13 µm length × I.D. 30 cm × 7.8 mm 808024
G2000PW particle size 10 µm length × I.D. 30 cm × 7.5 mm 805761
G2500PW particle size 10 µm length × I.D. 30 cm × 7.5 mm 808028
G3000PW particle size 10 µm length × I.D. 30 cm × 7.5 mm 805762
G4000PW particle size 17 µm length × I.D. 30 cm × 7.5 mm 805763
G5000PW particle size 17 µm length × I.D. 30 cm × 7.5 mm 805764
G6000PW particle size 17 µm length × I.D. 30 cm × 7.5 mm 805765
TSK-GEL
Column		 Particle Size (µm) Pore Size (Å) Sample MW
(PEGS/PEOs)		 Sample MW
Dextrans
G-Oligo-PW 6 125 <2,000 -
G2000PW 10 125 <2,000 -
G2500PWXL 6 <200 <3,000 -
G2500PW 10 <200 <3,000 -
G3000PWXL 6 200 <50,000 <60,000
G3000PW 10 200 <50,000 <60,000
G4000PWXL 10 500 2,000-300,000 1,000-700,000
G4000PW 17 500 2,000-300,000 1,000-700,000
G5000PWXL 10 1,000 4,000-1,000,000 50,000-7,000,000
G5000PW 17 100 4,000-1,000,000 4,000-1,000,000
G6000PWXL 13 >1,000 40,000-8,000,000 500,000- 50,000,000
G6000PW 17 >1,000 40,000-8,000,000 500,000-50,000,000
G-DNA-PW 10 4,000 40,000-8,000,000 -
GMPWXL 13 <100-1000 500-8,000,000 <50,000,000
GMPW 17 <100-1000 500-8,000,000 <50,000,000
Mobile Phase: Polyethylene glycols/polyethylene oxides—distilled water
Mobile Phase: Dextrans—0.2 M phosphate buffer, pH 6.8
Additional resources  
Title
TSK-GEL PW and PWXL Columns (71 Kb PDF) id. T494075
 
TSK-GEL HHR Series Gel Permeation Columns back to top
TSK-GEL H series gel permeation columns are stable in solvents having a wide range of polarities. The particles do not swell or shrink as the solvent is changed from toluene through methanol. However, these columns cannot be used with polar solvents, such as water or water:methanol mixtures. Spherical 5 µm polystyrene/divinylbenzene particles provide a minimum of 16,000 plates per 30 cm x 7.8 mm I.D. column. Eight pore sizes are available, ranging from an exclusion limit of about 2,000 Daltons for G1000HHR columns to more than 10,000,000 Daltons for G7000HHR columns. The four mixed bed columns (H, L, M, N) feature extended linear molecular weight operating ranges for sample screening or more formal analyses.
(see table after HXL columns for Analyte Molecular Weight Range for these columns)
Description Cat. No.
G1000HHR particle size 5 µm length × I.D. 30 cm × 7.8 mm 817352
G2000HHR particle size 5 µm length × I.D. 30 cm × 7.8 mm 817353
G2500HHR particle size 5 µm length × I.D. 30 cm × 7.8 mm 817354
G3000HHR particle size 5 µm length × I.D. 30 cm × 7.8 mm 817355
G4000HHR particle size 5 µm length × I.D. 30 cm × 7.8 mm 817356
G5000HHR particle size 5 µm length × I.D. 30 cm × 7.8 mm 817357
GMHHR-L particle size 5 µm length × I.D. 30 cm × 7.8 mm 817362
GMHHR-M particle size 5 µm length × I.D. 30 cm × 7.8 mm 817392
Guard Column  
HHR-L Guard column particle size 7 µm length × I.D. 4.0 cm × 6.0 mm1 817368
1 Use to protect G1000HHR - G4000HHR and GMHHR-L columns.
 
Additional resources  
Title
TSK-GEL HHR Columns (68 Kb PDF) id. T496085
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TSK-GEL HXL Series Gel Permeation Columns back to top
The TSK-GEL HHR and TSK-GEL HXL columns perform very similarly. The HXL columns were developed as an upgrade from the previous series, the TSK-GEL H. The HXL can be converted to different mobile phases for doing non-aqueous polymer separations, but it was found that the performance deteriorated when the conversion occurred. The HHR columns were developed to allow easy conversion between different mobile phases. The HHR is considered exceptionally stable, and is easily converted to other mobile phases with little loss in theoretical plates. The HXL columns are a good choice if your mobile phase of choice is THF and you do not plan on changing mobile phases. If you will change mobile phases or your mobile phase of choice is not THF, the HHR columns should be used.
Description Cat. No.
G1000HXL particle size 5 µm length × I.D. 30 cm × 7.8 mm 816131
G2000HXL particle size 5 µm length × I.D. 30 cm × 7.8 mm 816134
G2500HXL particle size 5 µm length × I.D. 30 cm × 7.8 mm 816135
G3000HXL particle size 6 µm length × I.D. 30 cm × 7.8 mm 816136
G4000HXL particle size 6 µm length × I.D. 30 cm × 7.8 mm 816137
G5000HXL particle size 9 µm length × I.D. 30 cm × 7.8 mm 816138
G6000HXL particle size 9 µm length × I.D. 30 cm × 7.8 mm 816139
GMHXL particle size 9 µm length × I.D. 30 cm × 7.8 mm 816141
GMHXL-HT particle size 13 µm length × I.D. 30 cm × 7.8 mm 807112
Guard Column  
HXL-L Guard column particle size 6 µm length × I.D. 4.0 cm × 6.0 mm1 807113
HXL-H Guard column particle size 13 µm length × I.D. 4.0 cm × 6.0 mm2 813727
1 Use to protect G1000HXL - G4000HXL columns.
2 Use to protect G5000HXL - G6000HXK columns.
Column Analyte Molecular
Weight Range (Daltons)
G1000H <1,500
G2000H <4,000
G2500H <1.2 x 104
G3000H <3.0 x 104
G4000H <5.5 x 105
G5000H <1.5 x 106
G6000H <~1 x 107
G7000H <~5 x 107
GMH-H <~1 x 107
GMH-L <1.0 x 104
GMH-M <1.0 x 106
TSK-GEL Reversed-Phase Columns back to top
 
Description Cat. No.
Silica-Based Column  
Oligo-DNA RP particle size 5 µm length × I.D. 15 cm × 4.6 mm 813352
Super ODS particle size 2 µm length × I.D. 5 cm × 4.6 mm 818154
Super ODS particle size 2 µm length × I.D. 10 cm × 4.6 mm 818197
Resin-Based Column  
C18-NPR particle size 2.5 µm length × I.D. 3.5 cm × 4.6 mm 814005
C18-4PW particle size 7 µm length × I.D. 15 cm × 4.6 mm 813351
Phenyl-5PW RP particle size 10 µm length × I.D. 7.5 cm × 4.6 mm 808043
Guard Filters  
Guard Filter 818207
Stand-Alone Holder for Filter 818206
TSK-GEL Affinity Columns back to top
High efficiency, resin-based TSK-GEL affinity columns (10 µm particles) separate or purify many enzymes and other proteins. The table below describes the characteristics of the columns offered.
 
Characteristics of TSK-GEL Affinity Columns
Immobilized
Ligand		 Adsorption Capacity
(per mL Gel)		 Typical Uses
ABA-5PW    
p-amino benzamidine 3-4 mg trypsin trypsin, bovine thrombin,
urokinase, enterokinase,
plasminogen activator
Boronate-5PW    
m-aminophenyl boronic acid 40 µmol sorbitol glycoproteins, nucleases,
nucleotides, catecholamines,
carbohydrates, transfer RNAs
Chelate-5PW    
iminodiacetic acid ~20 µmol
Cu+2 or Zn+2		 serum proteins, interferon,
collagenase, granule protein,
plasminogen activator, lactoferrin
Description Cat. No.
ABA-5PW particle size 10 µm length × I.D. 7.5 cm × 7.5 mm 813067
Boronate-5PW particle size 10 µm length × I.D. 7.5 cm × 7.5 mm 813066
Chelate-5PW particle size 10 µm length × I.D. 7.5 cm × 7.5 mm 808645
Guard Column Kit1  
Chelate-5PW particle size 20 µm length × I.D. 1 cm × 6.0 mm 808647
1 Guard column kit contains one cartridge, a stand-alone holder, 5 mL packing, 5 cm of 1/16 in. tubing, and 2 nuts and ferrules.
TSK-GEL Hydrophobic Interaction Columns back to top
Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.

TSK-GEL Ether-5PW, Phenyl-5PW, and Butyl-NPR resin-based columns provide a range of hydrophobicities for chromatographic optimization. Ammonium sulfate concentration can be minimized by using a very hydrophobic packing (e.g.,butyl). We recommend an Ether-5PW column for purifying very hydrophobic proteins. Ether-5PW and Phenyl- 5PW packings are based on TSK-GEL G5000PW resin - 10 µm particles with 1000 Å pores. Butyl-NPR packing is prepared from 2.5 µm nonporous particles, allowing rapid analyses. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
Proteins, mixture
Column:   TSK-GEL Phenyl-5PW, 7.5 cm × 7.5 mm I.D., 10 µm particles
Cat. No.:   807573
Mobile Phase:   0.1 M phosphate buffer, pH 7.0 plus (NH4)2SO4, 1.8 M to 0 M in 60 min
Flow Rate:   1 mL/min
Det.:   UV, 280 nm
  713-1025
Description Cat. No.
Butyl-5PW particle size 2.5 µm length × I.D. 3.5 cm × 4.6 mm 814947
Ether-5PW particle size 10 µm length × I.D. 7.5 cm × 7.5 mm 808641
Phenyl-5PW particle size 10 µm length × I.D. 7.5 cm × 7.5 mm 807573
Guard Column Kit1  
Phenyl-5PW particle size 20 µm length × I.D. 1 cm × 6.0 mm 807652
1 Guard column kit contains one cartridge, a stand-alone holder, 5 mL packing, 5 cm of 1/16 in. tubing, and 2 nuts and ferrules.
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