LENTICULE® discs

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What are LENTICULE® discs?
LENTICULE discs contain viable microorganisms in a certified and narrow defined quantity (ISO/IEC 17025), produced under reproducible conditions (ISO Guide 34). The bacterial and fungal strains are prepared directly from strains selected from PHE’s National Collection of Type Cultures (NCTC) and National Collection of Pathogenic Fungi (NCPF). The discs consist of bacteria or fungi in a solid water soluble matrix. Microorganisms in this form are stable for at least one year and are in a viable stage (no lag phase or recovery time). Each batch is provided with a comprehensive certificate of analysis that specifies the mean number of colony forming units (cfu), an expanded uncertainty about the mean value, details about the method used to determine the product data and the number of passages (subcultures) from the original strain.

Applications
  • QC to assure the quality of test results (water, food, beverage, environmental etc)
  • Performance testing of media acc. ISO 11133
  • Validation of new methods
  • Start material for Proficiency testing or ring trials
  • Method development
  • Staff training
  • Starter cultures

Storage: -20°C
Shipment wet ice! To ensure we maintain the highest product quality under all transport conditions we now ship Lenticules on wet ice. Short time at room temperature is no problem.

Use:
The product concentrations are designed in a range where no or only minimal dilution must be done. The discs dissolve in liquid media and on agar plates which means easy handling but it is also a very economical solution.
Most solid and liquid medium or rehydration buffer can be used. The disc can be rehydrated in as little as 100 μL, or in larger volumes, e.g. 100 mL medium. It is also possible to add the disc to a molten and cooled medium used for pour plate techniques. The rehydration process takes approximately 10 minutes. On solid media, the disc forms a droplet that can be spread with a sterile loop. In liquid media, the disc dissolves very quickly.

LENTICULE discs are manufactured by Sigma-Aldrich under license from Public Health England.

UKAS Accreditation
Public Health England is accredited in accordance with the recognised International Guide ISO Guide 34:2009 General requirements for the competence of reference material producers. UKAS certificate ISO Guide 34 2009

SAS Accreditation
Sigma-Aldrich is accredited in accordance with the recognised International Guide ISO Guide 34:2009 General requirements for the competence of reference material producers. SAS certificate ISO Guide 34 2009

See also our actual Certified Reference Microorganisms brochure.

Ordering
Please specify if you require a single batch - in the remark field when ordering (only for orders with more than 15 packs)

Use promo code SFV at checkout to receive a free sample. Offer valid until March 01, 2018 (limited to one pack per customer)