Biochemical Reagents for Identification

A diverse range of biochemical reagents are known for the identification of certain metabolisms and to differentiate between bacteria.
Classical biochemical tests are often used to identify microorganisms; the results are seen by color change. In most cases, detection is based on the reaction of an enzyme with a certain substrate. Also, methods to detect certain metabolites by chemical reaction and or complex building techniques are used. At the end, a color change gives a result that leads to greater cognition of the unknown organism.

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29333 Barritt’s Reagent A for microbiology VP1 Reagent Voges-Proskauer Test with Barritt’s Reagent A This reagent is used in Voges-Proskauer test for detection of acetoin production by bacterial cultures along with Barritt Reagent B.
39442 Barritt’s Reagent B for microbiology VP2 Reagent Voges-Proskauer Test with Barritt’s Reagent B This reagent is used in Voges-Proskauer test for detection of acetoin production by bacterial cultures along with Barritt Reagent A.
07770 N,N-Dimethyl-p-phenylenediamine dihydrochloride for microbiology, ≥99.0% 4-Amino-N,N-dimethylaniline dihydrochloride DMPD · 2HCl DMPPDA · 2HCl  
49825 DMACA Reagent for microbiology 4-(Dimethylamino)-cinnamaldehyde solution Used to determine the ability of an organism to split indole from the tryptophan molecule.
03891 Ehrlich’s solution 4-(Dimethylamino)benzaldehyde – hydrochloric acid solution reagent for urobilinogen
88597 Hydrogen peroxide solution 3%, for microbiology Catalase Test  
60983 Kovac’s reagent for indoles for microbiology 4-(Dimethylamino)benzaldehyde solution Indole reagent according to Kovac In the presence of oxygen, some bacteria, like E. coli, are able to split tryptophan into indole and α-aminopropionic acid. This reagent is for detecting the indole and identifying the indole-positive and indole-negative microorganisms.
67309 Kovac’s reagent for indoles for microbiology 4-(Dimethylamino)benzaldehyde solution Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.
08714 Methyl Red solution for microbiology   Some bacteria utilize glucose to form large amounts of acid with the result that the pH value of the medium falls distinct. Other species produce no or less free acid. This difference can be visualized by using methyl red.
39441 Nitrate Reagent B for microbiology 4-Aminobenzenesulfonic acid Sulfanilic acid solution α-Naphthylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphthylamine to form a red azo dye.
07689 O’Meara’s Reagent for microbiology Voges-Proskauer test with O’Meara Reagent The reagent is used in Voges-Proskauer test for the detection of acetoin production by bacterial cultures.
07345 Oxidase Reagent according to Gaby-Hadley A for microbiology Gaby-Hadley Reagent A The reagent is used for detection of oxidase activity of bacterial culture along with Oxidase Reagent B according to Gaby-Hadley.
07817 Oxidase Reagent according to Gaby-Hadley B for microbiology Gaby-Hadley Reagent B The reagent is used for detection of oxidase activity of a bacterial culture along with Oxidase Reagent A according Gaby-Hadley.
18502 Oxidase Reagent according to Gordon-McLeod for microbiology Gordon-McLeod Reagent The reagent is used for detection of oxidase activity of a bacterial culture.
80353 TDA Reagent for microbiology Ferric chloride solution Iron(III) chloride solution Tryptophan Deaminase Reagent For identification of Proteus species by detection of tryptophan deaminase activity.