A diverse range of biochemical reagents are known for the identification of certain metabolisms and to differentiate between bacteria. Classical biochemical tests are often used to identify microorganisms; the results are seen by color change. In most cases, detection is based on the reaction of an enzyme with a certain substrate. Also, methods to detect certain metabolites by chemical reaction and or complex building techniques are used. At the end, a color change gives a result that leads to greater cognition of the unknown organism.
Kovac’s reagent for indoles BioChemika, for microbiology
4-(Dimethylamino)benzaldehyde solution
In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.
4-(Dimethylamino)benzaldehyde solution Indole reagent according to Kovac
In the presence of oxygen, some bacteria, like E. coli, are able to split tryptophan into indole and α-aminopropionic acid. This reagent is for detecting the indole and identifying the indole-positive and indole-negative microorganisms.
Some bacteria utilize glucose to form large amounts of acid with the result that the pH value of the medium falls distinct. Other species produce no or less free acid. This difference can be visualized by using methyl red.
α-Naphthylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphthylamine to form a red azo dye.
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