Fluka and Supelco

Chromogenic Media

Culture media for the simple and fast detection of bacteria using chromogenic substrates. The chromogenic mixture contains chromogenic substrates as Salmon-GAL, X-Gal, X-glucuronide, etc. Certain enzymes, produced by some bacteria, cleave these substrates, resulting in the different coloration of certain bacteria colonies.

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94382 Candida Ident Agar, modified for microbiology   For the selective isolation and identification of Candida species from clinical material like stool, urine, skin scurf and swabs.
    • 12398 CP ChromoSelect Agar for microbiology CPC Agar Clostridium perfringens ChromoSelect Agar Clostridium perfringens Chromogen Agar  
    • 05662 HiCrome™ Aureus Agar Base for microbiology Staphylococcus aureus Agar HiCrome™ Aureus Agar Base HiCrome™ HiCrome Aureus Agar Base is recommended for the isolation and enumeration of coagulase positive Staphylococcus aureus from environment samples. Coagulase positive S. aureus gives brown-black colonies where as S. epidermidis gives yellow-slightly brownish colonies.
    • 81938 HiCrome™ Coliform Agar for microbiology Coliform Agar HiCrome™ HiCrome™ Coliform Agar is a selective chromogenic medium recommended for simultaneous detection of Escherichia coli and total coliforms in water and food samples. Sodium lauryl sulfate inhibits Gram-positive organisms. The chromogenic mixture contains two chromogenic substrates, Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction, thereby increasing detection reliability in combination with the two chromogens. To confirm E. coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.
    • 75605 HiCrome™ m-CP Agar Base for microbiology Membrane Clostridium perfringens HiCrome™ Agar Base m-CP HiCrome™ Agar Base The m-CP Agar Base with selective supplement is recommended by the Directive of the Council of the European Union 98/83/EC for isolation and enumeratin of Clostridium perfringens from water samples using membrane filtration technique.
    • 92324 HiCrome™ Cronobacter spp. Agar for microbiology Cronobacter spp. HiCrome™ Agar Enterobacter sakazakii HiCrome™ Agar HiCrome™ Enterobacter sakazakii Agar HiCrome™ Cronobacter spp Agar is recommended for isolation and identification of Cronobacter spp. (fomerly Enterobacter sakazakii) from food, water, sewage, urine and faeces samples.
    • 14703 HiCrome™ Cronobacter spp. Agar, modified for microbiology Cronobacter spp. isolation agar Enterobacter sakazakii HiCrome™ Agar, modified Enterobacter sakazakii isolation agar CSIA ESIA HiCrome™ Enterobacter sakazakii Agar, modified HiCrome™ Enterobacter sakazakii Agar modified meets the formulation recommended by ISO Committee for the isolation and identification of Enterobacter sakazakii from milk and milk products.
    • 73009 HiCrome™ ECC Agar for microbiology E. coli Coliform Agar HiCrome™ ECC Agar HiCrome™ HiCrome ECC Agar is a differential medium recommended for the presumptive identification of Escherichia coli and other coliforms in food and environmental samples. The chromogenic mixture containstwo chromogens as X-glucuronide and Salmon-GAL. X-glucuronide is cleaved by the enzyme beta glucuronidase produced by Escherichia coli. Salmon-GAL is cleaved by the enzyme galactosidase produced by majority of coliforns, including Escherichia coli. Color of E. coli colonies: blue/purple
    • 85927 HiCrome™ ECC Selective Agar for microbiology E. coli-Coliform Selective Agar HiCrome™ ECC Selective Agar HiCrome™ HiCrome™ ECC Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol® inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The chromogenic mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli, cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.
    • 39894 HiCrome™ EC O157 Agar EC O157 Agar HiCrome™ HiCrome EC O157 Agar is a chromogenic medium for isolation and differentiation of E. coli O157 from food and environmental samples.
    • 72557 HiCrome™ EC O157:H7 Selective Agar, Basis for microbiology EC O157:H7 Selektive Agar HiCrome™, Base HiCrome EC O157:H7 Selective Agar is recommended for selective isolation and easy detection of Escherichia coli O157:H7 from food samples.
    • 70722 HiCrome™ E. coli Agar B for microbiology E. coli Agar B HiCrome™ HiCrome E. coli Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.
    • 80330 HiCrome™ Enrichment Broth (Base) for EC 0157:H7 for microbiology EC 0157:H7 HiCrome™ Enrichment Broth (Base) HiCrome™ Enrichment Broth (Base) for EC 0157:H7 is recommended for isolation and selective differentiation of E. coli 0157:H7 from food and environmental samples by chromogenic method.
    • 52441 HiCrome™ Enterococci Broth for microbiology Enterococci HiCrome™ Broth HiCrome™ Enterococci Broth is recommended for identification and differentiation of Enterococci from water samples.
    • 90925 HiCrome™ Klebsiella Selective Agar Base for microbiology Klebsiella Selective HiCrome™ Agar Base HiCrome™ Klebsiella Selective Agar for selective isolation and easy detection of Klebsiella species from water and other sources. This medium can also be used in membrane filtration procedure.
    • 53707 HiCrome™ Listeria Agar Base, modified for microbiology Listeria HiCrome™ Agar Base, modified HiCrome™ Listeria Agar Base, modified is a selective and differential agar medium recommended for rapid and direct identification of Listeria species, specifically Listeria monocytogenes.
    • 83339 HiCrome™ MacConkey-Sorbitol Agar for microbiology MacConkey-Sorbitol Agar HiCrome™ SMAC Agar HiCrome™ Sorbitol MacConkey Agar HiCrome™ HiCrome™ MacConkey Sorbitol Agar is recommended for selective isolation of Escherichia coli 0157:H7 from food and animal feeding stuffs. MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. The medium contains sorbitol instead of lactose and it is recommended for the detection of enteropathogenic strains of E. coli 0157:H7 which ferments lactose but does not ferment sorbitol and hence produce colorless colonies. Sorbitol fermenting strains of E. coli produce pink-red colonies. The red color is due to production of acid from sorbitol, absorption of neutral red and a subsequent color change of the dye when pH of the medium falls below 6.8. E. coli 0157:H7 has been recognised as a cause of haemorrhagic colitis. B.C. indicator is added to detect the presence of an enzyme β-D-glucuronidase which is specific for E. coli. Stranins of E. coli possessing β-D-glucuronidase appear as blue colored colonies on the medium. Enteropathogenic strains of E. coli 0157 do not possess beta-D-glucuronidase activity and thus produce colorless colonies. E. coli fermenting sorbitol and possessing beta-D-glucuronidase activity produce purple colored colonies. Most of the gram-positive organisms are inhibited by crystal violet and bile salts. Addition of Tellurite-Cefixime Supplement makes the medium selective. Potassium Tellurite selects the serogroups 0157 from other E. coli serogroups and inhibits Aeromonas species and Providencia species. Cefixime inhibits Proteus species.
    • 90923 HiCrome™ MeReSa Agar Base for microbiology HiCrome™ Methicillin Resistant Staphylococcus aureus Agar Base MRSA Chromogen Agar HiCrome™ MeReSa Agar is recommended for the isolation and selective identification of Methicillin Resistant Staphylococcus aureus (MRSA) from clinical isolates.
    • 00563 HiCrome™ MM Agar for microbiology MM Agar HiCrome™ Miller and Mallinson Agar, HiCrome™ HiCrome MM Agar is recommended for identification and differentiation of Salmonella and non-salmonella like Citrobacter from water samples.
    • 90918 HiCrome™ RajHans Medium, modified for microbiology HiCrome™ Salmonella Medium, modified Salmonella RajHans Chromogen Medium, modified HiCrome™ RajHans Medium modified is recommended for identification and differentiation of Salmonella species from the members of Enterobacteriaceae, especially Proteus species.
    • 51489 HiCrome™ Rapid Coliform Broth for microbiology Coliform Rapid HiCrome™ Broth Rapid Coliform HiCrome™ Broth Rapid HiColiform Broth is used for detection and conformation of Escherichia coli and coliforms on the basics of enzyme substrate reaction from water samples, using a combination of chromogenic and fluorogenic substrate.
    • 51759 HiCrome™ Rapid Enterococci Agar for microbiology Enterococci Rapid HiCrome™ Agar Rapid Enterococci HiCrome™ Agar HiCrome™ Rapid Enterococci Agar is used for rapid and easy identification and differentiation of Enterococci from water samples. It contains chromogenic substrate, which aids in the detection of Enterococci from water samples.
    • 78419 HiCrome™ Salmonella Agar for microbiology Salmonella Agar HiCrome™ HiCrome Salmonella Agar is a selective medium used for simultaneous detection of Escherichia coli and Salmonella from food and water. Escherichia coli and Salmonella are easily distinguishable due to the colony characteristics. Salmonella give light purple colonies a halo. Escherichia coli has a characteristic blue color, other organisms give colorless colonies. The characteristic light purple and blue color is due to the chromogenic mixture.
    • 05538 HiCrome™ Salmonella Agar, Improved for microbiology Salmonella Agar HiCrome™, Improved A selective chromogenic medium used for simultaneous detection of Salmonella and Escherichia coli from food and water samples.
    • 90924 HiCrome™ m-TEC Agar for microbiology m-TEC HiCrome™ Agar HiCrome™ M-TEC Agar is recommended by the U.S. Environmental Protection Agency (USEPA) for differentiation and enumeration of thermotolerant E.coli from water by membrane filtration technique.
    • 16636 HiCrome™ UTI Agar, modified for microbiology UTI HiCrome™ Agar, modified Urinary Tract Infection Agar, modified HiCrome™ UTI Agar is chromogenic differential medium for identification, differentiation, and conformation of enteric bacteria from specimens such as urine, water, or food which may contain large number of Proteus species as well as potentially pathogenic Gram-positive organisms. Based on these characteristics, Modified HiCrome UTI Agar is suggested for use in place of MacConkey Agar.
    • 92323 HiCrome™ Vibrio Agar for microbiology Vibrio HiCrome™ Agar HiCrome™ Vibrio Agar is recommended for isolation, and selective chromogenic differentiation of Vibrio species from seafood.
    • 77408 Listeria mono Differential Agar (Base) for microbiology Agar Listeria Ottaviani & Agosti Listeria monocytogenes Differential Agar (Base) L. mono differential Agar has been recommended by ISO Committee for the selective and differential isolation of Listeria monocytogenes.
    • 39734 Membrane Lactose Glucuronide Agar for microbiology MLGA m-Lauryl Sulfate Chromogen Agar A chromogenic medium for the differentiation and enumeration of Escherichia coli and other coliforms, which simplifies the membrane filtration technique by reducing the number of filtration stages and the need for further confirmation steps. This medium is described in the Environment Agency′s report of UK, ′Methods for Examination of Waters and Associated Material - The Microbiology of Drinking Water (2002) - Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7).
    • 68879 Phenolphthalein phosphate agar for microbiology   Recommended for identification of phosphatase positive colonies of Staphylococcus aureus.
    • 84369 Salmonella Chromogen Agar for microbiology   A differential diagnostic agar for the detection of Salmonella in food and clinical material. A modification of the formulation described by Rambach (1990).
    • 01993 Salmonella Chromogen Agar Set for microbiology   A differential diagnostic agar for the detection of Salmonella in food and clinical material.
    • 92435 TBX Agar for microbiology Tryptone Bile X-glucuronide Agar Chromogenic Agar for the detection and enumeration of E. coli in foodstuffs, animal food and water without further confirmation. E. coli colonies are colored blue-green.