Culture media for the simple and fast detection of E. coli or other organisms using fluorescence substrates. E. coli, for example, can be detected using the MUG (glucuronidase) test and confirmed with a positive indole test. ß-D-glucoronidase, which is produced by E. coli, cleaves 4-Methylumbelliferyl-ß-D-glucuronide to 4-methylumbelliferone and glucuronide. The fluorogen 4-methylumbelliferone can be detected under a long wavelength UV lamp.
In the detection of E. coli and coliforms. Bile and brilliant green extensively inhibit the growth of accompanying flora, in particular gram-positive microorganisms. The presence of E. coli results in fluorescence in the UV. A positive indole test and possibly gas formation from lactose fermentation provide confirmation. The broth can be used in conjunction with the MPN method for E. coli and coliform enumeration in the water of bathing areas.
DEV Lactose Peptone MUG Broth BioChemika, for microbiology
For the enrichment and titre determination of coliform bacteria in connection with the bacteriological examination of water. The presence of E. coli can be demonstrated by fluorescence in the UV and a positive indole test.
The bile-salt mixture in this E. coli Direct Agar extensively inhibits the non-obligatory intestinal accompanying flora. Fluorescence in the UV and a positive indole test demonstrate the presence of E. coli in the colonies.
Rapid HiColiform Broth is used for detection and conformation of Escherichia coli and coliforms on the basics of enzyme substrate reaction from water samples, using a combination of chromogenic and fluorogenic substrate.
HiFluoro™ Pseudomonas Agar Base BioChemika, for microbiology
Pseudomonas Agar Base HiFluoro™
Hifluoro Pseudomonas Agar base is used as a selective medium for the isolation of Pseudomonas aeruginosa from pus, sputum, and drains etc. Pseudomonas aeruginosa breaks the fluorogenic compound to release the fluorogen which produces a visible fluorescence under long wave UV light.
For the isolation of Salmonella, Shigella and coliform bacteria, in particular E. coli, from diverse material. Bile salts and crystal violet extensively inhibit the Gram-positive flora. The presence of lactose and neutral red indicate lactose-positive colonies from which E. coli can be identified by fluorescence in the UV.
Selective medium for the detection and enumeration of coliform bacteria, in particular E. coli. Gram-positive accompanying flora are extensively inhibited by crystalviolet and bile salts. A color-change to red indicates lactose-positive colonies, within which E. coli can be demonstrated by fluorescence in the UV.
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