Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe gram-negative bacteria. They give negative Voges-Proskauer, indole and methyl red tests, but a positive catalase test. While some species show a negative reaction in the oxidase test, most species, including P. fluorescens, give a positive result. Another feature associated with Pseudomonas is the secretion of pyoverdin (fluorescein, a siderophore), a fluorescent yellow-green pigment under iron-limiting conditions. Certain Pseudomonas species may also produce additional pigments, such as pyocyanin (blue pigment, a siderophore) by P. aeruginosa, quinolobactin (yellow, dark green in presence of iron, a siderophore) by P. fluorescens, a reddish pigment called pyorubrin and pyomelanin (brown pigment). On blood agar a hemolytic reaction can be observed.

e.g. Pseudomonas aeruginosa: Primarily a soil bacteria, food poisoning, cause of various infections (e.g. burns, urinary and respiratory tract).

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B5051 Nonselective
Bushnell Haas broth for microbiology