SPE - 3M Empore™ Products SPE Cartridges

Empore SPE™ cartridges are packed with a PTFE membrane enmeshed with SPE particles. Layered above the SPE membrane is a polypropylene pre-filter that prevents particulates from reaching the underlying membrane. The dense particle packing and uniform distribution within the Empore membane offer outstanding extraction efficiency and reproducibility.

Ordering Information

Features & Benefits: Reduced sample and elution volumes Reduced time for eluate evaporation Increased sampling throughput No channeling effects High and reproducible recoveries

1 (coarse density), 2 (medium density), 3 (fine density, made up of 5 individual layers), 4 (porous PP support)

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Solvent Volume Guidelines for Empore SPE Cartridges
		Empore Catridge Sizes
Step	Solvent	4 mm/1 mL	7 mm/3 mL	10 mm/6 mL
Condition	Methanol Water	150 µL 300 µL	250 µL 500 µL	500 µL 1000 µL
Load	Sample Buffer/IS	250 µL 250 µL	1000 µL 1000 µL	2000 µL 2000 µL
Wash	Water Organic/Aqueous	300 µL 300 µL	500 µL 500 µL	1000 µL 1000 µL
Elute	Organic	100-150 µL	200-300 µL	600-800 µL
Notes: It is recommended to optimize the volume of elution solvent to ensure that the minimum volume is used that will elute the anlayte reproducibly from the sorbent phase. Due to the disruption of ionic interactions, which can be stronger than reversed-phase interactions, slightly more elution solvent volume may be required for mixed phase disks than for a reversed-phase sorbent.

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