Empore SPE™ cartridges are packed with a PTFE membrane enmeshed with SPE particles. Layered above the SPE membrane is a polypropylene pre-filter that prevents particulates from reaching the underlying membrane. The dense particle packing and uniform distribution within the Empore membane offer outstanding extraction efficiency and reproducibility.
Features & Benefits:
- Reduced sample and elution volumes
- Reduced time for eluate evaporation
- Increased sampling throughput
- No channeling effects
- High and reproducible recoveries
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1 (coarse density), 2 (medium density), 3 (fine density, made up of 5 individual layers), 4 (porous PP support) |
| Solvent Volume Guidelines for Empore SPE Cartridges |
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Empore Catridge Sizes |
| Step |
Solvent |
4 mm/1 mL |
7 mm/3 mL |
10 mm/6 mL |
| Condition |
Methanol
Water |
150 µL
300 µL |
250 µL
500 µL |
500 µL
1000 µL |
| Load |
Sample
Buffer/IS |
250 µL
250 µL |
1000 µL
1000 µL |
2000 µL
2000 µL |
| Wash |
Water
Organic/Aqueous |
300 µL
300 µL |
500 µL
500 µL |
1000 µL
1000 µL |
| Elute |
Organic |
100-150 µL |
200-300 µL |
600-800 µL |
| Notes: It is recommended to optimize the volume of elution solvent to ensure that the minimum volume is used that will elute the anlayte reproducibly from the sorbent phase. Due to the disruption of ionic interactions, which can be stronger than reversed-phase interactions, slightly more elution solvent volume may be required for mixed phase disks than for a reversed-phase sorbent. |
Our Empore Technical Library comprises of dozens of helpful resources, application examples, and an 80+ page application bibliography to facilitate efficient method development and troubleshooting.
