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Solid Phase Extraction
QuEChERS (Dispersive SPE)
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 The “QuEChERS” (Quick, Easy, Cheap, Effective, Rugged, and Safe) method, dispersive SPE (dSPE), is an emerging sample prep technique that is becoming increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products.
In the QuEChERS method, food/agricultural samples are first extracted with an aqueous miscible solvent (e.g., acetonitrile) in the presence of high amounts of salts (e.g., sodium chloride and magnesium sulfate) and/or buffering agents (e.g. citrate) to induce liquid phase separation and stabilize acid and base labile pesticides. Upon shaking and centrifugation, an aliquot of the organic phase is subjected to further clean up using SPE. Unlike traditional methods using SPE tubes, in dispersive SPE, clean up is facilitated by mixing bulk amounts of SPE (e.g., Supelclean PSA, ENVI-Carb, and/or Discovery DSC-18) with the extract. After sample clean up, the mixture is centrifuged and the resulting supernatant can either be analyzed directly or can be subjected to minor further treatment before analysis. |
| Custom Services |
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Supelco offers vials and centrifuge tubes in bulk quantities, each packed with pre-determined amounts of salts and SPE sorbents to support the most common method configurations used today. This saves the analyst the time taken to weigh out the individual materials multiple times.
Our Dispersive SPE Customization Service features:
- 50 mL centrifuge tubes
- 12 mL centrifuge tubes
- 2 mL microcentrifuge tubes
- Glass vials
- A wide selection of Sigma-Aldrich/Supelco salts, reagents, and SPE phases
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Fat Removal in Difficult Matrices
