Solid Phase Extraction

QuEChERS (Dispersive SPE)

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Introduction to Supel QuE Z-Sep
Supel™ QuE Z-Sep Fat Removal in Difficult Matrices
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For a trial, you may request free dSPE sample MultiPaks for method development.


dispersive spe tubesThe “QuEChERS” (Quick, Easy, Cheap, Effective, Rugged, and Safe) method, dispersive SPE (dSPE), is an emerging sample prep technique that is becoming increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products.

In the QuEChERS method, food/agricultural samples are first extracted with an aqueous miscible solvent (e.g., acetonitrile) in the presence of high amounts of salts (e.g., sodium chloride and magnesium sulfate) and/or buffering agents (e.g. citrate) to induce liquid phase separation and stabilize acid and base labile pesticides. Upon shaking and centrifugation, an aliquot of the organic phase is subjected to further clean up using SPE. Unlike traditional methods using SPE tubes, in dispersive SPE, clean up is facilitated by mixing bulk amounts of SPE (e.g., Supelclean PSA, ENVI-Carb, and/or Discovery DSC-18) with the extract. After sample clean up, the mixture is centrifuged and the resulting supernatant can either be analyzed directly or can be subjected to minor further treatment before analysis.


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Supelco offers vials and centrifuge tubes in bulk quantities, each packed with pre-determined amounts of salts and SPE sorbents to support the most common method configurations used today. This saves the analyst the time taken to weigh out the individual materials multiple times.

Our Dispersive SPE Customization Service features:
  • 50 mL centrifuge tubes
  • 12 mL centrifuge tubes
  • 2 mL microcentrifuge tubes
  • Glass vials
  • A wide selection of Sigma-Aldrich/Supelco salts, reagents, and SPE phases
Custom Services

Technical Resources back to top
Subject No. Pages File Size Id.
Watch an in-depth webinar on Advantages of QuEChERS by Steven Lehotay, co-inventor of this technique, via Separation Science (registration required) - - -
Increase Removal of Fat and Pigment from Avocado Extracts Prior to GC-MS Analysis of Pesticide and Metabolite Residue (Reporter US Volume 31.2) - - -
Dispersive SPE for QuEChERS Method (brochure) 4 1 Mb T411065
Improved Recovery of Carbendazim from Orange Juice Using QuEChERS Method - - -
Using a New High Capacity Lipid Depletion Material in Comparison to a C18 Adsorbent During Dispersive SPE Cleanup for Analysis of Veterinary Drugs in Animal Samples 21 237 Kb T411127
A New QuEChERS Dispersive SPE Material and Method for Analysis of Veterinary Drug Residue by LC-MS-MS 21 237 Kb T411098
Testing for Aflatoxins in Corn and Peanut Butter using QuEChERS Cleanup and LC Fluorescence 16 311 Kb T411097
Intralab Validation of the EN 15662 Method for the Determination of Pesticide Residues Using a Fused-Core™ Ascentis® Express RP-Amide HPLC Column by LC-MS/MS and Clean-up by Dispersive SPE (QuEChERS) (TheReporter Europe Vol. 39) 402 Kb   
Dispersive SPE (dSPE) Product Line Data/Instruction Sheet 2 37 Kb T706013
QuEChERS Multiresidue Method (overview chart) 2 218 Kb JWP
The Extraction & Analysis of Multi-Residue Pesticides in Oranges Using Dispersive SPE & GC-MS (poster) 20 196 Kb T406100
Extraction and Analyses of Agricultural Pesticides from Oranges Using the "QuEChERS" Method (TheReporter 24.3) 3 381 Kb T206003
NEW Custom “Dispersive” SPE Products for Pesticide and Acrylamide Analysis (TheReporter 23.4) 2 383 Kb T205004