SPE

HybridSPE-PPT “In-Well” 96-well Precipitation Visual

1. Precipitate Proteins by adding 100 µL plasma or serum to the HybridSPE-PPT plate followed by 300 µL 1% formic acid in acetonitrile. Add I.S. as necessary.

2. Mix by vortexing/shaking HybridSPE-PPT plate or by aspirating/ dispensing with 0.5-1 mL pipette tip (e.g., TOMTEC Quadra liquid handler)

3.

Apply vacuum.
The packed-bed filter/frit assembly acts as a depth filter for the concurrent physical removal of precipitated proteins and chemical removal phospholipids. Small molecules (e.g., pharma compounds and metabolites) pass through unretained.


4. Resulting filtrate/eluate is free of proteins and phospholipids and ready for immediate LC-MS-MS analysis; or it can be evaporated and reconstituted as necessary prior to analysis