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Solid Phase Extraction
HybridSPE-Phospholipid
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HybridSPE combines the simplicity of protein precipitation with the selectivity of Solid Phase Extraction (SPE) for the targeted removal of phospholipids and proteins in biological plasma/serum (Figure 1). The technology utilizes a zirconia-coated particle, and exhibits selective affinity towards phospholipids while remaining non-selective towards a range of basic, neutral and acidic compounds. The phospholipid retention mechanism is based on highly selective Lewis acid-base interaction between the proprietary zirconia ions (functionally bonded to the HybridSPE stationary phase) and the phosphate moiety consistent with all phospholipids (Figure 2). |
| Figure 1. HybridSPE “In-Well” Precipitation Method and Phospholipid Removal |
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View the details for each step in this process
- Precipitate Proteins
- Mix
- Apply vacuum
- Results
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| Figure 2. Lewis Acid-Base Interaction Between Hybrid SPE Zirconia Ions and Phospholipids |
Proprietary HybridSPE
Zirconia Coated Silica |
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The phosphate moiety of phospholipids
Is a strong Lewis base
(electron donor) that interacts with Zr
atoms coated on the silica surface. |
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| Ion-Suppression & Phospholipid Contamination |
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In pharmaceutical bioanalysis, researchers develop and run various assays to quantitate drugs, pharmaceutical candidates, and their metabolites in biological fluids such as serum and plasma. As analysts strive for faster analyses, shorter run times, and lower limits of detection, ion-suppression due to inadequate removal endogenous sample interferences has become of great concern. It is well documented that one of the principal causes of ion-suppression is phospholipid contamination. Not only is signal suppression often evident in the positive ion electrospray mode (+ESI), phospholipids can often remain on the analytical column after sample analysis, and elute uncontrollably in a given LC run sequence. |
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| Phospholipid Enrichment |
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HybridSPE-Phospholipid can also be used to selectively capture and elute phospholipids for analysis and phospholipid profiling. The same Lewis-acid-base interactions that selectively remove phospholipids as interferences can also be used to recover phospholipids for analysis. A strong Lewis-base (5% ammonium hydroxide in acetonitrile) can be used to effectively and efficiently elute retained phospholipids from the HybridSPE sorbent. |
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| Features & Benefits of HybridSPE: |
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- Merges both Protein Precipitation (PPT) & Solid Phase Extraction (SPE)
- Offers simplicity & generic nature of protein precipitation
- Selectivity approaches SPE via the targeted removal of phospholipids
- 2-3 step generic procedure
- 100% removal of phospholipids & precipitated proteins
- Minimal to no method development
- Available in 96-well and 1 mL cartridge dimensions
- Can be used to enrich phospholipids for analysis
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| Description |
Qty |
Cat. No. |
| HybridSPE Products |
| Precipitation 96-well Plate, 50 mg/well |
1 |
575656-U |
 Precipitation 96-well Plate, 15 mg/well |
1 |
52794-U |
| Precipitation Cartridge, 30 mg/1 mL |
100 |
55261-U |
| Precipitation Cartridge, 500 mg/6 mL |
30 |
55267-U |
| Related Products |
| 96-well Protein Precipitation Filter Plate |
1 |
55263-U |
| Supelco PlatePrep Vacuum Manifold |
1 |
57192-U |
| 96 Square/Deep Well Collection Plates, 0.35 mL, PP |
50 |
575651-U |
| 96 Square/Deep Well Collection Plates, 1 mL, PP |
50 |
575652-U |
| 96 Square/Deep Well Collection Plates, 2 mL, PP |
50 |
575653-U |
| 96 Square Well Pierceable Cap Mats |
50 |
575655-U |
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