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Solid Phase Extraction

Supel™-Select HLB SPE

Sample Prep Performance at the Price you Desire!

Supelco’s Supel-Select HLB SPE is a hydrophilic modified styrene based polymer developed for the solid phase extraction of a broad range of compounds from aqueous samples. The retention mechanism is predominately based on reversed-phase interaction. However, because the phase is hydrophilic modified, the phase is also selective for more polar compounds. Examples of more polar compounds that are retained and recovered on Supel-Select HLB include (but are not limited to): pyridoxine (logPo/w -0.56), riboflavin (logPo/w -2.02), biotin (logPo/w 0.11).

supel-select-hlb
Phase chemistry:  Hydrophilic modified styrene polymer
pH Compatibility:  0-14
Particle Size:  55-60 µm
MS friendly:  Yes
Surface area:  400-410 m2/g
Pore volume: 0.88 mL/g
Pore size: 87 Å

Request a FREE Supel-Select HLB SPE Sample Pack.

Feature Benefit
Hydrophilic modified styrene resin Extract and recover a broad range of analytes (polar to non-polar, acidic to basic) using a single sorbent
Generic methodology Save time, money, and headache during method development
Greater capacity Allows for smaller bed weights = smaller elution volumes = time savings in sample processing
Resistant to overdrying More robust methodology
Low UV and MS extractables Lower background for greater sensitivity
Stringent production & QC Greater lot-to-lot, tube-to-tube, and well-to-well consistency for improved accuracy and precision

Minimal Extractables

Supel-Select HLB SPE phases are quality controlled for both LC-UV and LC-MS/MS extractables. As assays require greater sensitivity, analytical “cleanliness” of both SPE phases and associated hardware becomes more critical.
In this simple test, acetonitrile was passed through both Supel-Select HLB SPE tubes and the leading competitor’s tubes followed by LC-UV analysis.

Supel-Select HLB SPE imparted less than 25% of UV extractables than the leading competitor.


Supel Select HLB vs Competitor

Ordering Information
Description Qty/Pk Cat. No.
Supel-Select HLB SPE
  30 mg/1 mL 100 54181-U
  60 mg/3 mL 50 54182-U
  200 mg/6 mL 30 54183-U
  500 mg/12 mL 20 54184-U
  1 g/20 mL 20 54186-U
Supel-Select HLB 96-well SPE
  10 mg/ well 1 Inquire
  30 mg /well 1 575661-U
  60 mg/ well 1 575662-U


Technical Resources
Description Type Size
(Kb)
No.
Pages
I.D.
Supel-Select HLB SPE Instruction /Data Sheet & Troubleshooting Guide Data Sheet 59 2 T708013
Supel-Select HLB SPE Brochure Brochure 650 6 T408148