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Analytical / Chromatography
Thin Layer Chromatography Equipment Offering
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Sigma-Aldrich's offering for thin layer chromatography (TLC) and high performance TLC (HPTLC) includes plates, reagents and accessories. Our glass, PET (polyester) foils, and aluminum foil TLC plates are available coated with silica gel (unmodified, nano-silica gel, C-18, C-18 chiral-modified, amino, cyano), aluminum oxide (Alox), cellulose (fibers, microcrystalline) and Polyamide 6 stationary phases, with and without indicator.
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TLC Plates
TLC Spray Reagents
TLC Reagent Sprayers
TLC Development Tanks
View all our available TLC Accessories in the online product catalog.
View all our available products for TLC and HPTLC in the online product catalog.
On-demand webinar: Difficult samples? Simply separated! TLC/HPTLC for rapid quantitative results in Food & Beverage
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TLC & HPTLC Plates - Ordering Information
Stationary Phases:
- Silica gel (unmodified, nano silica gel, RP-18, RP-18 chiral modified, amino, cyano)
- Aluminium oxide (Alox)
- Cellulose (fibers, microcrystalline)
- Polyamide 6
Supports:
- Glass
- PET (polyester) foils
- Aluminium foils
In the following table you may find a sampling of some of the products we recently added to our TLC product range. For a detailed listing, view Ordering Information or contact your local Sigma-Aldrich office. |
| Catalog No.
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Absorbent / Support
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Pkg
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Dimensions
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Indicator
254 nm
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Layer
Thickness
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Medium
Pore Diameter
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| 71532
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Aluminum oxide on TLC-PET foils |
25 ea
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20x 20cm
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yes
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0.2mm
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60 Å
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| 89071
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Aluminum oxide on TLC-PET foils |
50 ea
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4x8cm
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yes
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0.2mm
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60 Å
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| 94039
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Aluminum oxide on TLC-PET foils |
25 ea
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20x 20cm
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no
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0.2mm
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60 Å
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| 90066
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Aluminum oxide on TLC-glass plates |
25 ea
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20x20 cm
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yes
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0.25mm
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60 Å
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| 72375
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Cellulose fibers on TLC-PET foils |
25 ea
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20x 20cm
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yes
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0.1mm
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n/a
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| 78536
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Cellulose fibers on TLC-PET foils |
25 ea
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20x20 cm
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no
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0.1mm
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n/a
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| 89153
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Cellulose fibers on TLC-PET foils |
50 ea
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4x8 cm
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no
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0.1mm
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n/a
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| 73128
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Cellulose fibers on TLC-glass plates |
25 ea
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20x20 cm
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yes
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0.1mm
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n/a
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| 75382
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Cellulose fibers on TLC-glass plates |
25 ea
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20x20 cm
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no
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0.1mm
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n/a
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| 89937
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Cellulose fibers on TLC-glass plates |
50 ea
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10x20 cm
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yes
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0.1mm
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n/a
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| 95412
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Cellulose fibers on TLC-glass plates |
50 ea
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10x20 cm
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no
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0.1mm
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n/a
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| 95413
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Cellulose fibers TLC-Cards on aluminum foils |
50 ea
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20x20 cm
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no
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0.1mm
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n/a
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| 95559
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Cellulose fibers TLC-Cards on aluminum foils |
50 ea
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4x8 cm
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no
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0.1mm
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n/a
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| 73127
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Cellulose microcrystalline on TLC-PET foils |
25 ea
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20x20 cm
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no
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0.1mm
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n/a
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| 95725
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Cellulose microcrystalline on TLC-PET foils |
25 ea
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20x20 cm
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yes
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0.1mm
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n/a
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| 76131
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Silica gel on TLC-PET foils |
1 ea
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500x20 cm
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yes
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0.2mm
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60 Å
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| 89070
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Silica gel on TLC-PET foils |
50 ea
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4x 8cm
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yes
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0.25mm
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60 Å
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| 70643
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Silica gel on TLC-Cards on aluminum foils |
50 ea
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4x 8cm
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yes
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0.2mm
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60 Å
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| 70644
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Silica gel on TLC-Cards on aluminum foils |
50 ea
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5x10 cm
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no
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0.2mm
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60 Å
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| 91835
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Silica gel on TLC-Cards on aluminum foils |
50 ea
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5x10 cm
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yes
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0.2mm
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60 Å
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| TLC Spray Reagents - Ordering Information |
To visualize the colorless components on a TLC plate you must either use a plate with a fluorescent indicator and observe the plate under ultraviolet illumination to see the samples or your can also use a second method for visualizing the sample spots on a TLC plate: Spray Reagents. These reagents are mixtures of chemicals that, when sprayed on the plate and allowed to react, will cause colorless compounds to change to colored compounds.
The following tables give you an overview of the most frequenty used spray reagents and their applications.
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Compound Index
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| Aflatoxins |
Fast Blue B Salt |
| Aliphatic Aldehydes |
3-Methyl-2-benzothiazolinone hydrazonehydrochloride |
| Alkaloids |
Dragendorff's Reagent, Potassium hexachloroplatinate, Phosphomolybdic Acid, Zinc chloride |
| Amines |
Potassium hexachloroplatinate, Ehrlich’s Reagent, Ninhydrin, 5-Methoxyninhydrin Monohydrate |
| Antibiotics |
Ehrlich's Reagent, Ninhydrin |
| Amino Acids, Related Compounds |
Ninhydrin, Folin's Reagent |
| Antioxidants |
Phosphomolybdic Acid Solution |
| Barbiturates |
2,6-Dichlorophenolindophenol Sodium salt Hydrate, Mercuric chloride |
| Base Metals (Pb, Zn, Cd, Cr, Cu, Fe, Mo) |
Diphenylcarbazone |
| Bile Acids |
Potassium dichromate |
| Cyanides |
2,6-Dibromoquinone-4-chloroimide |
| Dienes |
Tetracyanoethylene |
| Flavanoids and Foodstuffs |
2-Aminoethyl diphenylborinate, Bromocresol Green Solution, Antimony (III) chloride |
| Glycolipids |
Orcine |
| Glycosids |
Lead tetraacetate |
| Glycine |
4-Chloro-7-nitrobenzofurazan |
| Halogens |
Chloramine T Trihydrate |
| Hexylamine |
5-Dimethylaminonaphthalene-1-sulfonylchloride, 2-Methoxy-2,4-diphenyl-3(2H)-furanone |
| Indole Derivatives |
Ehrlich's Reagent |
| Iron |
2,2'-Bipyridyl |
| Lipids |
Antimony Trichloride, Bromothymol Blue, Dragendorff’s Reagent, Molybdenum Blue, Ninhydrin, Orcine, Rhodamine B |
| Nickel |
Dimethylglyoxime |
| Nitrate and Nitrite |
N-(1-Naphthyl)ethylenediamine dihydrochloride Monomethanolate |
| Organic Acids |
Bromocresol Green Solution |
| Osazones |
N,N,N',N'-Tetramethyl-1,4-phenylenediam. dihydrochloride |
| Phenols |
Chloranil, 2,6-Dibromoquinone-4-chloroimide, Phosphomolybdic Acid |
| Precious metals (Cu, Ag, Au, Hg, Pt) |
5-(4-Dimethylaminobenzylidene)rhodanine |
| Steroids |
Antimony (III) chloride, Ehrlich’sReagent, Phosphomolybdic Acid |
| Sugars and Derivatives |
Ninhydrin, 2',7'-Dichlorofluorescein, Lead(II) acetate basic |
| Terpenes |
Phosphomolybdic Acid Solution |
| Urobilinogene |
5-(4-Dimethylaminobenzylidene)rhodanine |
| Vitamins |
Antimony Trichloride, Potassium hexachloroplatinate |
| Cat.No.
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Name
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Description
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Package size
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| 42810
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2-Aminoethyl diphenylborinate |
>97.0% (NT), for detection of flavones |
1g, 5g, 25g |
| 28213
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Ammonium sulfate Reagent |
~10% (T) in ethanol/water |
100 ml |
| 21623
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Antimon(III) chloride Reagent (free of CHC) |
~30% Sb, for detection of vitamins |
100 ml |
| 75240
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Bial’s Reagent |
~98% (GC), for detection of glycolipids |
10g, 50g, 250g |
| 14454
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2,2'-Bipyridyl |
>99.0% (NT), for detection of Fe |
5g, 25g, 100g |
| 02559
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Bromocresol Green Solution |
ready-to-use, for the detection of flavanoids and foodstuff |
500 ml |
| 23270
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Chloramine T Trihydrate |
>98.0% (RT), for detection of halogens and bromate |
50g, 250g |
| 23280
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Chloranil |
>99.0% (AT), for detection of phenols |
10g, 50g, 250g |
| 25455
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4-Chloro-7-nitrobenzofurazan |
>99.0% (TLC), for detection of glycine |
1g, 5g, 25g |
| 34080
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2,6-Dibromoquinone-4-chloroimide |
>98.0 5 (AT), for the detection of phosphatase, cyanides and phenols |
10g, 50g |
| 02591
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2',7'-Dichlorofluorescein Solution |
ready-to-use, for detection of sweeteners |
500 ml |
| 36180
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2,6-Dichlorophenolindophenol Sodium salt Hydrate |
>97.0% (AT), for detection of N-substituted barbiturates |
1g, 5g, 25g |
| 35620
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2,6-Dichloroquinone-4-chloroimide |
>99.0% (AT), for detection of vitamin B6 |
10g, 50g |
| 70382
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3,4-Dihydro-3,4-dioxo-1-naphthalenesulfonic acid Sodium salt |
>97.0% (T), Folin’s reagent, for detection of amines and amino acids |
10g, 50g |
| 39070
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4-Dimethylaminobenzaldehyde |
>99.0% (HPLC), for detection of hydroxyproline |
10g, 50g, 250g |
| 02560
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4-(Dimethylamino)benzaldehyde Solution |
ready-to-use, Ehrlich's reagent, for detection of amines |
500 ml |
| 39090
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5-(4-Dimethylaminobenzylidene)rhodanine |
>98.0% (S), for detection of Ag, Au, Cu, Hg, Pd, Pt, Alkaloids and Urobilinogen |
10g, 50g |
| 39220
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5-Dimethylaminonaphthalene-1-sulfonylchloride |
>99.0% (HPLC), for detection of hexylamine |
1g, 5g, 50g |
| 40390
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Dimethylglyoxime |
>99.0% (TLC), for the detection of Ni |
100g |
| 42870
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Diphenylcarbazone |
~97% (HPLC) for detection of Hg, Pb, Zn, Cd, Cr, Cu, Fe, Hg, Mo |
5g, 25g |
| 44578
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Dragendorff Reagent |
ready-to-use, for detection of nitrogen compounds |
100 ml |
| 44660
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Fast Blue B Salt, for microscopy |
Standard, for detection of aflatoxins |
5g, 25g, 100g |
| 02554
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Fluorescence Indicator green 254 nm |
for thin layer chromatography |
50g, 250g |
| 15317
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Lead(II) acetate basic |
>72% Pb (KT), for detection of sugar |
100 g, 500 g |
| 15370
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Lead tetraacetate |
>95%, for detection of vicinal diols and glycosids |
25g |
| 63536
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Manganese(II) chloride Tetrahydrate |
>99.0% (KT), for detection of organothio-phosphorous compounds |
250g, 1kg |
| 83366
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Mercuric chloride |
>99.0%, for detection of barbiturates |
100g, 500 g |
| 64958
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2-Methoxy-2,4-diphenyl-3(2H)-furanone |
>98.0% (HPLC), for detection of hexylamine |
25mg, 100mg |
| 65128
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5-Methoxyninhydrin Monohydrate |
>93% (HPLC), for detection of amines |
250 mg, 1g |
| 65875
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3-Methyl-2-benzothiazolinone hydrazonehydrochloride |
>99.0% (T), MBTH; for detection of aliphatic aldehydes |
2.5g, 10g, 50g |
| 69870
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Morin |
Standard, for the detection of Al, Be, Zn, Ga, In, Sc |
10g, 50g |
| 70720
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N-(1-Naphthyl)ethylenediamine dihydrochloride Monomethanolate |
>99.0% (AT), for detection of nitrate and nitrite |
5g, 25g, 100g |
| 72489
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Ninhydrin |
~99% (UV), for detection aof amino acids, amines and aminosugars |
250g, 1kg |
| 17975
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Ninhydrin Reagent |
ready-to-use, according to Stahl |
100ml |
| 75240
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Orcine Monohydrate |
~98% (GC), for detection of glycolipids |
10g, 50g, 250g |
| 02553
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Phosphomolybdic acid Solution |
ready-to-use, for detection of alkaloids |
100ml, 500 ml |
| 22260
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Phosphoric acid Reagent |
~20% (T) in ethanol/water |
100ml |
| 79760
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Phthaldialdehyde, for fluorescence |
>99.0% (HPLC), for detection of amino acids |
1g, 5g, 50g |
| 60190
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Potassium dichromate |
>99.5% (RT), for detection of bile acids and lipids |
50g, 250g |
| 60260
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Potassium hexachloroplatinate(IV) |
puriss.; ~40% Pt, for detection of alkaloids |
1g, 5g |
| 02558
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Rhodamine B Solution, ready-to-use |
ready-to-use, for detection of lipids |
100ml, 500 ml |
| 86090
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Sulfanilic acid |
>99.0%, for detection of nitrites |
5g, 100 g, 500 g |
| 87120
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Tetracyanoethylene |
>97.0% (N), for dtection of dienes |
10g, 50, 250g |
| 87890
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N,N,N',N'-Tetramethyl-1,4-phenylenediam. dihydrochloride |
>97.0% (AT), for detection of osazones |
5g, 25g |
| 88960
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Thiooxine hydrochloride |
~97% (CHN), for determination of metal ions |
1g, 5g |
| 02652
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Dyestuff Mixture I for TLC, package w.20 ml |
for thin layer chromatography |
1pc |
| 89580
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o-Tolidine |
>98.0% (NT), for the detection of Au, Ce, Cl, Mn and free halogens |
25g, 100 g, 500g |
| 89760
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Toluene-4-sulfonic acid Monohydrate |
~99% (T), for acetyl detection |
100g, 500 g |
| 93140
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2,3,5-Triphenyltetrazolium chloride |
>99.0% (AT), reducing sugars |
10g, 50g |
| 96470
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Zinc chloride |
>98.0% (KT), for detection of alkaloids |
500 g, 1kg |
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| Tube Type
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Flask Type
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| Compact size offers good plate visibility and minimal solvent use. Can be used as a staining chamber. |
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