Analytical / Chromatography

Thin Layer Chromatography Equipment Offering

Sigma-Aldrich's offering for thin layer chromatography (TLC) and high performance TLC (HPTLC) includes plates, reagents and accessories. Our glass, PET (polyester) foils, and aluminum foil TLC plates are available coated with silica gel (unmodified, nano-silica gel, C-18, C-18 chiral-modified, amino, cyano), aluminum oxide (Alox), cellulose (fibers, microcrystalline) and Polyamide 6 stationary phases, with and without indicator.
TLC Plates
TLC Spray Reagents
TLC Reagent Sprayers
TLC Development Tanks 


View all our available TLC Accessories in the online product catalog.

View all our available products for TLC and HPTLC in the online product catalog.

View
/ Request
TLC Brochure

TLC & HPTLC Plates - Ordering Information
Stationary Phases:
  • Silica gel (unmodified, nano silica gel, RP-18, RP-18 chiral modified, amino, cyano)
  • Aluminium oxide (Alox)
  • Cellulose (fibers, microcrystalline)
  • Polyamide 6

Supports:
  • Glass
  • PET (polyester) foils
  • Aluminium foils
In the following table you may find a sampling of some of the products we recently added to our TLC product range. For a detailed listing, view Ordering Information or contact your local Sigma-Aldrich office.
Catalog No.
Absorbent / Support
Pkg
Dimensions
Indicator
 254 nm
Layer
Thickness
Medium
Pore Diameter
71532
Aluminum oxide on TLC-PET foils
25 ea
20x 20cm
yes
0.2mm
60 Å
89071
Aluminum oxide on TLC-PET foils
50 ea
4x8cm
yes
0.2mm
60 Å
94039
Aluminum oxide on TLC-PET foils
25 ea
20x 20cm
no
0.2mm
60 Å
90066
Aluminum oxide on TLC-glass plates
25 ea
20x20 cm
yes
0.25mm
60 Å
72375
Cellulose fibers on TLC-PET foils
25 ea
20x 20cm
yes
0.1mm
n/a
78536
Cellulose fibers on TLC-PET foils
25 ea
20x20 cm
no
0.1mm
n/a
89153
Cellulose fibers on TLC-PET foils
50 ea
4x8 cm
no
0.1mm
n/a
73128
Cellulose fibers on TLC-glass plates
25 ea
20x20 cm
yes
0.1mm
n/a
75382
Cellulose fibers on TLC-glass plates
25 ea
20x20 cm
no
0.1mm
n/a
89937
Cellulose fibers on TLC-glass plates
50 ea
10x20 cm
yes
0.1mm
n/a
95412
Cellulose fibers on TLC-glass plates
50 ea
10x20 cm
no
0.1mm
n/a
95413
Cellulose fibers TLC-Cards on aluminum foils
50 ea
20x20 cm
no
0.1mm
n/a
95559
Cellulose fibers TLC-Cards on aluminum foils
50 ea
4x8 cm
no
0.1mm
n/a
73127
Cellulose microcrystalline on TLC-PET foils
25 ea
20x20 cm
no
0.1mm
n/a
95725
Cellulose microcrystalline on TLC-PET foils
25 ea
20x20 cm
yes
0.1mm
n/a
76131
Silica gel on TLC-PET foils
1 ea
500x20 cm
yes
0.2mm
60 Å
89070
Silica gel on TLC-PET foils
50 ea
4x 8cm
yes
0.25mm
60 Å
70643
Silica gel on TLC-Cards on aluminum foils
50 ea
4x 8cm
yes
0.2mm
60 Å
70644
Silica gel on TLC-Cards on aluminum foils
50 ea
5x10 cm
no
0.2mm
60 Å
91835
Silica gel on TLC-Cards on aluminum foils
50 ea
5x10 cm
yes
0.2mm
60 Å


TLC Spray Reagents - Ordering Information

To visualize the colorless components on a TLC plate you must either use a plate with a fluorescent indicator and observe the plate under ultraviolet illumination to see the samples or your can also use a second method for visualizing the sample spots on a TLC plate: Spray Reagents. These reagents are mixtures of chemicals that, when sprayed on the plate and allowed to react, will cause colorless compounds to change to colored compounds.

The following tables give you an overview of the most frequenty used spray reagents and their applications.

 

Compound Index
Aflatoxins Fast Blue B Salt
Aliphatic Aldehydes 3-Methyl-2-benzothiazolinone hydrazonehydrochloride
Alkaloids Dragendorff's Reagent, Potassium hexachloroplatinate, Phosphomolybdic Acid, Zinc chloride
Amines Potassium hexachloroplatinate, Ehrlich’s Reagent, Ninhydrin, 5-Methoxyninhydrin Monohydrate
Antibiotics Ehrlich's Reagent, Ninhydrin
Amino Acids, Related Compounds Ninhydrin, Folin's Reagent
Antioxidants Phosphomolybdic Acid Solution
Barbiturates 2,6-Dichlorophenolindophenol Sodium salt Hydrate, Mercuric chloride
Base Metals (Pb, Zn, Cd, Cr, Cu, Fe, Mo) Diphenylcarbazone
Bile Acids Potassium dichromate
Cyanides 2,6-Dibromoquinone-4-chloroimide
Dienes Tetracyanoethylene
Flavanoids and Foodstuffs 2-Aminoethyl diphenylborinate, Bromocresol Green Solution, Antimony (III) chloride
Glycolipids Orcine
Glycosids Lead tetraacetate
Glycine 4-Chloro-7-nitrobenzofurazan
Halogens Chloramine T Trihydrate
Hexylamine 5-Dimethylaminonaphthalene-1-sulfonylchloride, 2-Methoxy-2,4-diphenyl-3(2H)-furanone
Indole Derivatives Ehrlich's Reagent
Iron 2,2'-Bipyridyl
Lipids Antimony Trichloride, Bromothymol Blue, Dragendorff’s Reagent, Molybdenum Blue, Ninhydrin, Orcine, Rhodamine B
Nickel Dimethylglyoxime
Nitrate and Nitrite N-(1-Naphthyl)ethylenediamine dihydrochloride Monomethanolate
Organic Acids Bromocresol Green Solution
Osazones N,N,N',N'-Tetramethyl-1,4-phenylenediam. dihydrochloride
Phenols Chloranil, 2,6-Dibromoquinone-4-chloroimide, Phosphomolybdic Acid
Precious metals (Cu, Ag, Au, Hg, Pt) 5-(4-Dimethylaminobenzylidene)rhodanine
Steroids Antimony (III) chloride, Ehrlich’sReagent, Phosphomolybdic Acid
Sugars and Derivatives Ninhydrin, 2',7'-Dichlorofluorescein, Lead(II) acetate basic
Terpenes Phosphomolybdic Acid Solution
Urobilinogene 5-(4-Dimethylaminobenzylidene)rhodanine
Vitamins Antimony Trichloride, Potassium hexachloroplatinate

 

Cat.No.
Name
Description
Package size
42810
2-Aminoethyl diphenylborinate >97.0% (NT), for detection of flavones 1g, 5g, 25g
28213
Ammonium sulfate Reagent ~10% (T) in ethanol/water 100 ml
21623
Antimon(III) chloride Reagent (free of CHC) ~30% Sb, for detection of vitamins 100 ml
75240
Bial’s Reagent ~98% (GC), for detection of glycolipids 10g, 50g, 250g
14454
2,2'-Bipyridyl >99.0% (NT), for detection of Fe 5g, 25g, 100g
02559
Bromocresol Green Solution ready-to-use, for the detection of flavanoids and foodstuff 500 ml
23270
Chloramine T Trihydrate >98.0% (RT), for detection of halogens and bromate 50g, 250g
23280
Chloranil >99.0% (AT), for detection of phenols 10g, 50g, 250g
25455
4-Chloro-7-nitrobenzofurazan >99.0% (TLC), for detection of glycine 1g, 5g, 25g
34080
2,6-Dibromoquinone-4-chloroimide >98.0 5 (AT), for the detection of phosphatase, cyanides and phenols 10g, 50g
02591
2',7'-Dichlorofluorescein Solution ready-to-use, for detection of sweeteners 500 ml
36180
2,6-Dichlorophenolindophenol Sodium salt Hydrate >97.0% (AT), for detection of N-substituted barbiturates 1g, 5g, 25g
35620
2,6-Dichloroquinone-4-chloroimide >99.0% (AT), for detection of vitamin B6 10g, 50g
70382
3,4-Dihydro-3,4-dioxo-1-naphthalenesulfonic acid Sodium salt >97.0% (T), Folin’s reagent, for detection of amines and amino acids 10g, 50g
39070
4-Dimethylaminobenzaldehyde >99.0% (HPLC), for detection of hydroxyproline 10g, 50g, 250g
02560
4-(Dimethylamino)benzaldehyde Solution ready-to-use, Ehrlich's reagent, for detection of amines 500 ml
39090
5-(4-Dimethylaminobenzylidene)rhodanine >98.0% (S), for detection of Ag, Au, Cu, Hg, Pd, Pt, Alkaloids and Urobilinogen 10g, 50g
39220
5-Dimethylaminonaphthalene-1-sulfonylchloride >99.0% (HPLC), for detection of hexylamine 1g, 5g, 50g
40390
Dimethylglyoxime >99.0% (TLC), for the detection of Ni 100g
42870
Diphenylcarbazone ~97% (HPLC) for detection of Hg, Pb, Zn, Cd, Cr, Cu, Fe, Hg, Mo 5g, 25g
44578
Dragendorff Reagent ready-to-use, for detection of nitrogen compounds 100 ml
44660
Fast Blue B Salt, for microscopy Standard Fluka, for detection of aflatoxins 5g, 25g, 100g
02554
Fluorescence Indicator green 254 nm for thin layer chromatography 50g, 250g
15317
Lead(II) acetate basic >72% Pb (KT), for detection of sugar 100 g, 500 g
15370
Lead tetraacetate >95%, for detection of vicinal diols and glycosids 25g
63536
Manganese(II) chloride Tetrahydrate >99.0% (KT), for detection of organothio-phosphorous compounds 250g, 1kg
83366
Mercuric chloride >99.0%, for detection of barbiturates 100g, 500 g
64958
2-Methoxy-2,4-diphenyl-3(2H)-furanone >98.0% (HPLC), for detection of hexylamine 25mg, 100mg
65128
5-Methoxyninhydrin Monohydrate >93% (HPLC), for detection of amines 250 mg, 1g
65875
3-Methyl-2-benzothiazolinone hydrazonehydrochloride >99.0% (T), MBTH; for detection of aliphatic aldehydes 2.5g, 10g, 50g
69870
Morin Standard Fluka, for the detection of Al, Be, Zn, Ga, In, Sc 10g, 50g
70720
N-(1-Naphthyl)ethylenediamine dihydrochloride Monomethanolate >99.0% (AT), for detection of nitrate and nitrite 5g, 25g, 100g
72489
Ninhydrin ~99% (UV), for detection aof amino acids, amines and aminosugars 250g, 1kg
17975
Ninhydrin Reagent ready-to-use, according to Stahl 100ml
75240
Orcine Monohydrate ~98% (GC), for detection of glycolipids 10g, 50g, 250g
02553
Phosphomolybdic acid Solution ready-to-use, for detection of alkaloids 100ml, 500 ml
22260
Phosphoric acid Reagent ~20% (T) in ethanol/water 100ml
79760
Phthaldialdehyde, for fluorescence >99.0% (HPLC), for detection of amino acids 1g, 5g, 50g
60190
Potassium dichromate >99.5% (RT), for detection of bile acids and lipids 50g, 250g
60260
Potassium hexachloroplatinate(IV) puriss.; ~40% Pt, for detection of alkaloids 1g, 5g
02558
Rhodamine B Solution, ready-to-use ready-to-use, for detection of lipids 100ml, 500 ml
86090
Sulfanilic acid >99.0%, for detection of nitrites 5g, 100 g, 500 g
87120
Tetracyanoethylene >97.0% (N), for dtection of dienes 10g, 50, 250g
87890
N,N,N',N'-Tetramethyl-1,4-phenylenediam. dihydrochloride >97.0% (AT), for detection of osazones 5g, 25g
88960
Thiooxine hydrochloride ~97% (CHN), for determination of metal ions 1g, 5g
02652
Dyestuff Mixture I for TLC, package w.20 ml for thin layer chromatography 1pc
89580
o-Tolidine >98.0% (NT), for the detection of Au, Ce, Cl, Mn and free halogens 25g, 100 g, 500g
89760
Toluene-4-sulfonic acid Monohydrate ~99% (T), for acetyl detection 100g, 500 g
93140
2,3,5-Triphenyltetrazolium chloride >99.0% (AT), reducing sugars 10g, 50g
96470
Zinc chloride >98.0% (KT), for detection of alkaloids 500 g, 1kg


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TLC Reagent Sprayers - Ordering Information

 

Tube Type
Flask Type
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TLC Developing Tanks - Ordering Information
Compact size offers good plate visibility and minimal solvent use. Can be used as a staining chamber.
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