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Ascentis HPLC Columns

This 23-minute web seminar describes Supelco's Ascentis line of HPLC columns with emphasis on the RP separation mode. Ascentis columns are retentive, high performance phases that are designed specifically for separation of small molecules. Method development is made easy because all Ascentis columns have comparable retention. Since column screening normally does not require a mobile phase change, method development is rapid and easily automated.

 
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Introduction to Ascentis HPLC Columns
Part 1
Introduction & Selectivity Considerations

(12:46)
Part 2
Ascentis
RP-Amide

(5:20)
Part 3
Phenyl Polar RP Columns

(5:00)
Part 4
HILIC Mode & Summary

(1:38)

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Seminar Abstract

The web seminar covers the following broad topics:

  • Introduction & selectivity considerations with HPLC stationary phases
  • Ascentis RP-Amide phase
  • Phenyl polar RP columns
  • Selectivity comparisons, using various examples

Presenter Profile

Richard Henry received his B.S. degree in Chemistry from Juniata College in 1963 and Ph.D. in Analytical Chemistry from The Pennsylvania State University in 1966. After a postdoctoral year in separations at Purdue University with Professor L. B. Rogers, he joined DuPont in 1967 at the Experimental Station in Wilmington, DE and became one of the first employees of the DuPont Instrument Products Division in 1969. Dick worked closely with Dr. Jack Kirkland and others in the development of HPLC columns and packing materials while at DuPont. After about 10 years with Spectra-Physics and consulting on the west coast, he joined the Penn State University chemistry faculty in 1985 where he was Director of Analytical Laboratories and taught Instrumental Analysis to chemistry majors. In 1985, he also founded Keystone Scientific, Inc. where he developed HPLC columns and related separation technology. He retired from both Penn State University and Keystone Scientific in 2002, and remains active teaching short courses on separation technology and as a consultant. Dick has research interests in separation mechanisms and all applications of new LC column technology. He is also interested in new analytical techniques, especially the rapidly growing fields of UHPLC, chiral HPLC, LC-MS, and multidimensional separation methods. He served two terms as Chairman of the ACS Subdivision on Separations (1998-2002) and has also served for many years on its Executive Committee.

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