Specification Sheet
Product Name TNF-α human,
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested
Product Number SRP3177
Product Brand SIGMA
 
TEST SPECIFICATION
Appearance lyophilized powder
Color white to off-white
Purity by SDS-Page >=98%
Purity by HPLC >=98%
Endotoxin Level <= 1 EU/ug
Biological Activity The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is <= 0.05 ng/ml, corresponding to a specific activity of >= 2 x 107 units/mg.