A0166
 |  |  |
 |
Image
Useful Links & Tools
A0166
Sigma
Ampicillin sodium salt
cell culture tested, powder
| Synonym: | |
| CAS Number: | 69-52-3 |
| Linear Formula: | C16H18N3NaO4S |
| Molecular Weight: | 371.39 |
| Beilstein Registry Number: | 4119211 |
| EC Number: | 200-708-1 |
| MDL number: | MFCD00064313 |
| PubChem Substance ID: | 24890405 |
Customers Also Purchased
K1377 Kanamycin sulfate from Streptomyces kanamyceticus cell culture tested, powder | I6758 Isopropyl β- ≥99% (TLC), ≤0.1% Dioxane | D2650 Dimethyl sulfoxide ≥99.7%, Hybri-Max™, sterile-filtered, hybridoma tested | T7660 Tetracycline hydrochloride powder, cell culture tested |
Description
| Application | Recommended for antibacterial use in cell culture media at 100 mg/L. Recommended for use in ampicillin-resistance studies at 20-125 μg/ml. Stable at 37 °C for 3 days. |
| Biochem/physiol Actions | A β-lactam antibiotic with an amino group side chain attached to the penicillin structure. Penicillin derivative that inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner surface of the bacterial cell membrane. Bactericidal only to growing Escherichia coli . Mode of resistance: Cleavage of β-lactam ring of ampicillin by β-lactamase. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria. |
Properties
| form | powder |
| potency | ≥845 μg per mg |
| color | white with slight yellow cast |
| mp | 215 °C (dec.)(lit.) |
| solubility | H2O: 50 mg/mL (Stock solutions should be stored at −20 °C.) |
| suitability | cell culture tested |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xi |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| WGK Germany | 2 |
| RTECS | XH8400000 |
References
| reference | Raleigh, E.A., Ausubel, M., et al. Short Protocols in Molecular Biology 2nd ed., New York, NY , (1992) 12, 2 |
| Perlman, D., et al., Use of antibotics in cell culture. Meth. Enzymol. LVIII, 112, (1979) | |
| Merck | Merck 13,591 |
| Corp MSDS 1 (1), 247:C / RegBook 1 (2), 2065:J / Sax 6, 2410 / Sigma FT-IR 1 (1), 619:B / Structure Index 1, 328:C:3 |
