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A5955
Sigma
Antibiotic Antimycotic Solution (100×), Stabilized
sterile-filtered, with 10,000 units penicillin, 10 mg streptomycin and 25 μg amphotericin B per mL, cell culture tested
| MDL number: | MFCD00130520 |
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Description
| Application | Recommended for use in cell culture applications at 10 ml/L. |
| Biochem/physiol Actions | Mode of Action: Penicillin acts by inhibiting bacterial cell-wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding. Amphotericin B interferes with fungal membrane permeability by forming channels in the membranes and causing small molecules to leak out. Antimicrobial spectrum: Gram-negative bacteria, Gram-positive bacteria, fungi and yeasts. |
| Other Notes | Solubilized in a proprietary citrate buffer. Formulated to contain 10,000 units/ml penicillin G, 10 mg/ml streptomycin sulfate and 25 μg/ml amphotericin B. |
Properties
| sterility | sterile-filtered |
| form | suspension |
| total impurities | Endotoxin, tested |
| color | yellow |
| suitability | cell culture tested |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
References
| reference | Perlman, D., Jakoby, W. and Pastan I.H., Use of antibiotics in cell culture media, in. Meth. Enzymol. New York, NY 113, 112, (1979) |
| , Reynolds, J.E.F., ed. Martindale: The Extra Pharmacopoeia 31th ed., Great Britain , (1996), 181 | |
| Alberts, B, et al., Basic Genetic Mechanisms. Molecular Biology of the Cell 3rd ed., (New York, NY , (1994), 240 | |
| Dawson, R.M.C., et al. Data for Biochemical Research 3rd ed., New York , (1986), 297 | |
| , Reynolds, J.E.F., ed. Martindale: The Extra Pharmacopoeia 31th ed., Great Britain , (1996), 400 |
