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P3351
Sigma
Protein L–Agarose from Peptostreptococcus magnus
recombinant, expressed in Escherichia coli
Description
| Application | Protein L from Peptostreptococcus magnus binds immunoglobulins (Ig) primarily through kappa light chain interactions without interfering with the antigen binding site. Recombinant Protein L contains four Ig-binding domains. |
| Preparation Note | Prepared with recombinant Peptostreptococcus magnus Protein L. |
Properties
| recombinant | expressed in Escherichia coli |
| extent of labeling | 1-2 mg per mL |
| matrix | Cross-linked 4% beaded agarose |
| matrix activation | cyanogen bromide |
| matrix attachment | amino |
| capacity | 3-10 mg/mL binding capacity |
| storage temp. | 2-8°C |
Safety
| Hazard Codes | Xi |
| Risk Statements | 10-36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 1 |
| Flash Point(F) | 100 °F |
| Flash Point(C) | 38 °C |
References
| reference | Bjorck, L., Protein L. A novel bacterial cell wall protein with affinity for Ig L chains. J. Immunol. 140, 1194-1197, (1988) Abstract |
| Kastern, W. et al., Structure of peptostreptococcal Protein L and identification of a repeated immunoglobulin light chain-binding domain. J. Biol. Chem. 267, 12820-12825, (1992) Abstract |
