S3401
Sigma
Sample Buffer, Laemmli 2× Concentrate
Description
| Application | Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. |
| Other Notes | Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. |
| Quantity | Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well. |
Properties
| packaging | vial of 6 mL |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter |
| Hazard Codes | T |
| Risk Statements | 22-24-36/37-41 |
| Safety Statements | 26-36/37/39-45-61 |
| WGK Germany | 3 |
References
| reference | Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, (1970) Abstract |
| Wu M., et al. , Aging-associated dysfunction of Akt/protein kinase B: S-nitrosylation and acetaminophen intervention. PLoS ONE 7th ed., 4, e6430, (2009) Abstract | |
| Wu M., et al., Acetaminophen prevents aging-associated hyperglycemia in aged rats: effect of aging-associated hyperactivation of p38-MAPK and ERK1/2 Diabetes Metab. 3rd ed., 25, 279-86, (2009) |
