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Methods in molecular medicine

Preparation of soluble extracts from adenovirus-infected cells for studies of RNA splicing.


PMID 17656773

Abstract

Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro using T7 RNA polymerase-derived splicing substrate RNAs.

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