EMAIL THIS PAGE TO A FRIEND

Die Pharmazie

Extractive spectrophotometric determination of TRODAT-1 hydrochloride in lyophilized kit.


PMID 18819514

Abstract

A simple, sensitive, and accurate spectrophotometric method has been developed for the assay of TRODAT-1 hydrochloride in lyophilized kit. The method is based on the formation of ion-pair association complex of TRODAT-1 with bromothymol blue (BTB) in disodium hydrogen phosphate/citric acid buffer of pH 4.0. The colored product was extracted with chloroform, and measured spectrophotometrically at 414 nm. Beer's law was obeyed in the range of 5-25 microg/ml with molar absorptivity of 2.75 x 10(4) l/mol/cm. Optimization of experimental conditions was described for the method. The proposed method has been successfully applied for the analysis of TRODAT-1 hydrochloride in lyophilized kit. No interference with pharmaceutical excipients was observed.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

114413
Bromothymol Blue, ACS reagent, Dye content 95 %
C27H28Br2O5S
34656
Bromothymol Blue, pkg of 200 mL
C27H28Br2O5S