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Bioorganic & medicinal chemistry letters

Amino acid precursors for the detection of transketolase activity in Escherichia coli auxotrophs.


PMID 19535247

Abstract

Probes were developed for the in vivo detection of transketolase activity by the use of a complementation assay in Escherichia coli auxotrophs They combine the d-threo ketose moiety recognised by transketolase and the side chain of leucine or methionine. These compounds were donor substrates of yeast transketolase leading to the release of the corresponding alpha-hydroxyaldehydes which could be converted in E. coli by a cascade of reactions into leucine or methionine required for cellular growth.

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68138 Transketolase from E. coli, ≥0.1 U/mg