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Chemical & pharmaceutical bulletin

A chemiluminogenic substrate for N-acetyl-beta-D-glucosaminidase, o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide.


PMID 2393956

Abstract

A new type of chemiluminogenic substrate for N-acetyl-beta-D-glucosaminidase, o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide, was prepared by incorporating an enzyme-removable substituent, N-acetyl-D-glucosaminide group, into the hydrazide moiety of luminol. This substrate releases luminol upon enzymatic hydrolysis. The enzyme was detected chemiluminescently using this substrate.