EMAIL THIS PAGE TO A FRIEND

Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging

Optical glucose analogs of aminolevulinic acid for fluorescence-guided tumor resection and photodynamic therapy.


PMID 24323192

Abstract

We have developed and tested a novel conjugation of the clinically used prodrug aminolevulinic acid with 2-deoxyglucosamine as a novel probe (ALA-2DG) for fluorescence imaging and photodynamic therapy. ALA-2DG was successfully synthesized, and the mechanisms of probe uptake, PpIX synthesis, and photodynamic therapy efficacy were evaluated in vitro and in vivo. ALA-2DG led to PpIX synthesis in tumor cells in vitro and in tumor in vivo. Competitive and inhibitory assays in vitro showed a reduction of this PpIX synthesis that was not observed when cells were incubated with ALA itself, indicating that intracellular uptake of ALA-2DG occurs by GLUT-mediated active transport. Initial photodynamic therapy studies confirmed the efficacy of ALA-2DG as a photodynamic sensitizer. The in vitro assays suggest that ALA-2DG is taken up by cells via glucose transporters. Initial studies in oral cancer demonstrated the applicability of ALA-2DG for in vivo imaging and its potential as an alternative to ALA-PpIX-based fluorescence diagnostics and photodynamic therapy, providing higher tumor specificity.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

P7912
Phloretin, ≥99%
C15H14O5
91897
Phloretin, analytical reference material
C15H14O5