EMAIL THIS PAGE TO A FRIEND

Kidney international

Fructokinase activity mediates dehydration-induced renal injury.


PMID 24336030

Abstract

The epidemic of chronic kidney disease in Nicaragua (Mesoamerican nephropathy) has been linked with recurrent dehydration. Here we tested whether recurrent dehydration may cause renal injury by activation of the polyol pathway, resulting in the generation of endogenous fructose in the kidney that might subsequently induce renal injury via metabolism by fructokinase. Wild-type and fructokinase-deficient mice were subjected to recurrent heat-induced dehydration. One group of each genotype was provided water throughout the day and the other group was hydrated at night, after the dehydration. Both groups received the same total hydration in 24 h. Wild-type mice that received delayed hydration developed renal injury, with elevated serum creatinine, increased urinary NGAL, proximal tubular injury, and renal inflammation and fibrosis. This was associated with activation of the polyol pathway, with increased renal cortical sorbitol and fructose levels. Fructokinase-knockout mice with delayed hydration were protected from renal injury. Thus, recurrent dehydration can induce renal injury via a fructokinase-dependent mechanism, likely from the generation of endogenous fructose via the polyol pathway. Access to sufficient water during the dehydration period can protect mice from developing renal injury. These studies provide a potential mechanism for Mesoamerican nephropathy.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

HPA029298
Anti-ACE antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
F0127
D-(−)-Fructose, ≥99%
C6H12O6
F2543
D-(−)-Fructose, BioXtra
C6H12O6
F3510
D-(−)-Fructose, BioReagent, suitable for cell culture, suitable for insect cell culture
C6H12O6
F9048
D-(−)-Fructose, meets USP testing specifications
C6H12O6
F2793
D-(−)-Fructose, analytical standard, analytical standard for fructose assay kit, for use with enzymatic assay kit FA20
C6H12O6
47739
D-(−)-Fructose, BioUltra, ≥99.0% (HPLC)
C6H12O6
47740
D-(−)-Fructose, for microbiology, ≥99.0%
C6H12O6
47748
D-(−)-Fructose, tested according to Ph.Eur.
C6H12O6
1286504
Fructose, United States Pharmacopeia (USP) Reference Standard
C6H12O6
F0550000
Fructose, European Pharmacopoeia (EP) Reference Standard
C6H12O6
PHR1002
Fructose, Pharmaceutical Secondary Standard; Certified Reference Material
C6H12O6
RAB0054
Human MCP-1 / CCL2 ELISA Kit, for serum, plasma, cell culture supernatant and urine
WH0001636M1
Monoclonal Anti-ACE antibody produced in mouse, clone 6A4, purified immunoglobulin, buffered aqueous solution
SAB1403725
Monoclonal Anti-ACE antibody produced in mouse, clone 4B10, purified immunoglobulin, buffered aqueous solution
RAB0055
Mouse MCP-1 / CCL2 ELISA Kit, for serum, plasma and cell culture supernatant
RAB0056
Mouse MCP-1 / CCL2 ELISA Kit, for cell and tissue lysates
RAB0057
Rat MCP-1 / CCL2 ELISA Kit, for serum, plasma and cell culture supernatant
RAB0058
Rat MCP-1 / CCL2 ELISA Kit, for cell and tissue lysates