EMAIL THIS PAGE TO A FRIEND

International journal of oncology

Effect of a nutrient mixture on matrix metalloproteinase-9 dimers in various human cancer cell lines.


PMID 24378964

Abstract

Strong clinical and experimental evidence demonstrates association of elevated levels of matrix metalloproteinase MMP-9 with cancer progression, metastasis and shortened patient survival, as it plays a key role in tumor cell invasion and metastasis by digesting the basement membrane and ECM components. MMP-9 is secreted in both the monomeric and dimeric form. Although there is little research on MMP-9 dimers, some studies have shown the dimer to be associated with more aggressive tumor progression. Our objective was to study the relative secretion patterns of MMP-9 monomer and dimer in a variety of cancer cell lines and the effect of a nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract on MMP-9 secretion. The cancer cell lines were grown in their respective media, supplemented with 10% FBS, penicillin (100xa0U/ml) and streptomycin (100xa0µg/ml) in 24-well tissue culture plates. At near confluence, the cells were treated with NM at 0,10, 50, 100, 500 and 1000xa0µg/ml. Parallel sets of cultures were treated with PMA (100xa0ng/ml) for induction of MMP-9. Cell MMP-9 secretion was assayed by gelatinase zymography. MMP-9 dimer secretion patterns of cancer cells fell into different categories. We observed no MMP-9 dimer in prostate DU-145 and PC-3, pancreatic MIA-Pa-Ca2, colon HCT-116, bladder T-24, head and neck FaDu, glioblastoma A-172, T-98 and LN-18 and leukemia HL-60, Jurkat, and Raji cell lines. MMP-dimer secretion only with PMA induction was seen in breast MCF-7 and MDA-MB-231, uterine SK-UT-1, lung A-549, tongue SC-25, melanoma A2058, osteosarcoma U-2OS, rhabdomyosarcoma, fibrosarcoma HT-1080, chondrosarcoma SW-1350 and liposarcoma SW-872. Cervical HeLa and DoTcxa02xa04510, renal 786-0 and HCC SK-Hep-1 cells exhibited MMP-9 dimer without PMA treatment and increased secretion with PMA treatment. Sarcomas had the highest levels of MMP-9 monomer and dimer with and without PMA among these cancer cell lines. Cervical, uterine and male breast cancer cell lines showed the next highest levels of MMP-9, followed by breast cancer cell lines. Melanoma, renal, lung, head and neck and HCC showed lower levels and prostate, glioblastoma, bladder and leukemia cell lines the lowest. NM showed dose-dependent inhibition of MMP-9 monomer and dimer in all cell lines tested. In conclusion, high MMP-9 and dimer secretion levels correlated with the most aggressive cancer cell lines. NM was effective in inhibiting MMP-9 and dimer secretion in all cell lines tested, suggesting its therapeutic potential as an antimetastatic agent.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

23128
L-Lysine, analytical standard
C6H14N2O2
62850
L-Lysine solution, purum, 50% in H2O
C6H14N2O2
1568506
L-Proline, United States Pharmacopeia (USP) Reference Standard
C5H9NO2
93693
L-Proline, certified reference material, TraceCERT®
C5H9NO2
PHR1332
L-Proline, Pharmaceutical Secondary Standard; Certified Reference Material
C5H9NO2
266167
Manganese, chips, thickness <2.0 mm, 99%
Mn
266132
Manganese, powder, −325 mesh, ≥99% trace metals basis
Mn
463728
Manganese, powder, ≥99.9% trace metals basis
Mn
GF74177736
Manganese, foil, not light tested, 25x25mm, thickness 0.01mm, permanent polyester support, 98.7%
Mn
GF99005193
Manganese, foil, not light tested, 25x25mm, thickness 0.015mm, permanent polyester support, 98.7%
Mn
GF90559794
Manganese, foil, not light tested, 25x25mm, thickness 0.02mm, permanent polyester support, 98.7%
Mn
GF03428625
Manganese, foil, not light tested, 25x25mm, thickness 0.05mm, permanent polyester support, 98.7%
Mn
GF31519975
Manganese, foil, not light tested, 50x50mm, thickness 0.001mm, permanent polyester support, 98.7%
Mn
GF60677646
Manganese, foil, not light tested, 50x50mm, thickness 0.0025mm, permanent polyester support, 98.7%
Mn
GF64815239
Manganese, foil, not light tested, 50x50mm, thickness 0.002mm, permanent polyester support, 98.7%
Mn
GF41418378
Manganese, foil, not light tested, 50x50mm, thickness 0.003mm, permanent polyester support, 98.7%
Mn
GF33640860
Manganese, foil, not light tested, 50x50mm, thickness 0.004mm, permanent polyester support, 98.7%
Mn
GF85367605
Manganese, foil, not light tested, 50x50mm, thickness 0.005mm, permanent polyester support, 98.7%
Mn
GF87981564
Manganese, foil, not light tested, 50x50mm, thickness 0.006mm, permanent polyester support, 98.7%
Mn
GF53022833
Manganese, foil, not light tested, 50x50mm, thickness 0.007mm, permanent polyester support, 98.7%
Mn
GF71274258
Manganese, foil, not light tested, 50x50mm, thickness 0.008mm, permanent polyester support, 98.7%
Mn
GF70055524
Manganese, foil, not light tested, 50x50mm, thickness 0.009mm, permanent polyester support, 98.7%
Mn
GF86610539
Manganese, foil, not light tested, 50x50mm, thickness 0.0125mm, permanent polyester support, 98.7%
Mn
GF00832081
Manganese, foil, not light tested, 50x50mm, thickness 0.015mm, permanent polyester support, 98.7%
Mn
GF61911288
Manganese, foil, not light tested, 50x50mm, thickness 0.01mm, permanent polyester support, 98.7%
Mn
GF94645233
Manganese, foil, not light tested, 50x50mm, thickness 0.025mm, permanent polyester support, 98.7%
Mn
GF05710430
Manganese, foil, not light tested, 50x50mm, thickness 0.02mm, permanent polyester support, 98.7%
Mn
GF27632055
Manganese, foil, not light tested, 50x50mm, thickness 0.05mm, permanent polyester support, 98.7%
Mn
GF11021094
Manganese, rod, 10mm, diameter 4.0mm, cast, 99.5%
Mn
GF34573697
Manganese, rod, 35mm, diameter 2.0mm, cast, 99.5%
Mn