EMAIL THIS PAGE TO A FRIEND

Journal of dermatological science

Resveratrol inhibition of human keratinocyte proliferation via SIRT1/ARNT/ERK dependent downregulation of aquaporin 3.


PMID 24726500

Abstract

Aquaporin 3 (AQP3) is the predominant aquaporin in the skin and is overexpressed in hyperplastic epidermal disorders. Upregulation of AQP3 contributes to keratinocyte proliferation and epidermal hyperplasia. Resveratrol, a natural polyphenol, has an anti-proliferative effect on normal human epidermal keratinocytes (NHEKs), but its exact mechanism remains largely unknown. To investigate the ability and mechanism of resveratrol to affect the proliferation and the AQP3 expression in NHEKs. NHEKs treated with resveratrol were analyzed. BrdU incorporation assay, real-time PCR, Western blotting and RNA interference using small interfering RNA were employed. At non-toxic concentrations (less than 40μM), resveratrol inhibited the proliferation of NHEKs. Resveratrol inhibited the ERK phosphorylation and the AQP3 expression with reciprocal upregulation of ARNT expression in a concentration-dependent manner. The inhibitory effects of resveratrol on the ERK phosphorylation and the AQP3 expression were canceled by transfection of siRNA for ARNT, but not by that for AhR. Furthermore, the induction effect of resveratrol on ARNT expression was canceled after SIRT1 was knocked down in NHEKs. Resveratrol inhibited NHEK proliferation by downregulating the expression of AQP3 in an SIRT1/ARNT/ERK dependent fashion. This novel mechanism may facilitate drug innovation for hyperplastic skin disorders.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB5200111
Anti-AQP3 antibody produced in rabbit, 1 mg/mL, affinity isolated antibody
HPA014924
Anti-AQP3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB4504579
Anti-phospho-EGFR (pSer1026) antibody produced in rabbit, affinity isolated antibody
SAB4300062
Anti-phospho-EGFR (pSer1070) antibody produced in rabbit, affinity isolated antibody
SAB4504382
Anti-phospho-EGFR (pSer1070) antibody produced in rabbit, affinity isolated antibody
SAB4504580
Anti-phospho-EGFR (pSer1071) antibody produced in rabbit, affinity isolated antibody
SAB4504578
Anti-phospho-EGFR (pSer695) antibody produced in rabbit, affinity isolated antibody
SAB4300151
Anti-phospho-EGFR (pThr678) antibody produced in rabbit, affinity isolated antibody
SAB4503759
Anti-phospho-EGFR (pThr678) antibody produced in rabbit, affinity isolated antibody
SAB4300152
Anti-phospho-EGFR (pThr693) antibody produced in rabbit, affinity isolated antibody
SAB4503760
Anti-phospho-EGFR (pThr693) antibody produced in rabbit, affinity isolated antibody
SAB4503909
Anti-phospho-EGFR (pTyr1016) antibody produced in rabbit, affinity isolated antibody
SAB4504170
Anti-phospho-EGFR (pTyr1069) antibody produced in rabbit, affinity isolated antibody
SAB4300063
Anti-phospho-EGFR (pTyr1092) antibody produced in rabbit, affinity isolated antibody
SAB4504383
Anti-phospho-EGFR (pTyr1092) antibody produced in rabbit, affinity isolated antibody
SAB4300209
Anti-phospho-EGFR (pTyr1110) antibody produced in rabbit, affinity isolated antibody
SAB4504384
Anti-phospho-EGFR (pTyr1110) antibody produced in rabbit, affinity isolated antibody
SAB4300177
Anti-phospho-EGFR (pTyr1172) antibody produced in rabbit, affinity isolated antibody
SAB4504385
Anti-phospho-EGFR (pTyr1172) antibody produced in rabbit, affinity isolated antibody
SAB4300285
Anti-phospho-EGFR (pTyr1197) antibody produced in goat, affinity isolated antibody
SAB4300183
Anti-phospho-EGFR (pTyr1197) antibody produced in rabbit, affinity isolated antibody
SAB4504386
Anti-phospho-EGFR (pTyr1197) antibody produced in rabbit, affinity isolated antibody
SAB4300184
Anti-phospho-EGFR (pTyr869) antibody produced in rabbit, affinity isolated antibody
SAB4504387
Anti-phospho-EGFR (pTyr869) antibody produced in rabbit, affinity isolated antibody
E6154 Anti-phospho-Epidermal Growth Factor Receptor (pTyr1068) antibody produced in rabbit, affinity isolated antibody, buffered aqueous glycerol solution
E1530 Anti-phospho-Epidermal Growth Factor Receptor (pTyr1086) antibody produced in rabbit, affinity isolated antibody, buffered aqueous glycerol solution
E6279 Anti-phospho-Epidermal Growth Factor Receptor (pTyr1173) antibody produced in rabbit, affinity isolated antibody, buffered aqueous glycerol solution
E6029 Anti-phospho-Epidermal Growth Factor Receptor (pTyr845) antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution
E0907 Anti-phospho-Epidermal Growth Factor Receptor (pTyr845) antibody produced in rabbit, affinity isolated antibody, lyophilized powder
E1780 Anti-phospho-Epidermal Growth Factor Receptor (pTyr992) antibody produced in rabbit, affinity isolated antibody, buffered aqueous glycerol solution