EMAIL THIS PAGE TO A FRIEND

PloS one

MicroRNA-338 inhibits growth, invasion and metastasis of gastric cancer by targeting NRP1 expression.


PMID 24736504

Abstract

NRP1 as multifunctional non-tyrosine-kinase receptors play critical roles in tumor progression. MicroRNAs (miRNAs) are an important class of pervasive genes that are involved in a variety of biological functions, particularly cancer. It remains unclear whether miRNAs can regulate the expression of NRP1. The goal of this study was to identify miRNAs that could inhibit the growth, invasion and metastasis of gastric cancer by targeting NRP1 expression. We found that miR-338 expression was reduced in gastric cancer cell lines and in gastric cancer tissues. Moreover, we found that miR-338 inhibited gastric cancer cell migration, invasion, proliferation and promoted apoptosis by targeting NRP1 expression. As an upstream regulator of NRP1, miR-338 directly targets NRP1. The forced expression of miR-338 inhibited the phosphorylation of Erk1/2, P38 MAPK and Akt; however, the expression of phosphorylated Erk1/2, P38 MAPK and Akt was restored by the overexpression of NRP1. In AGS cells infected with miR-338 or transfected with SiNRP1, the protein levels of fibronectin, vimentin, N-cadherin and SNAIL were decreased, but the expression of E-cadherin was increased. The expression of mesenchymal markers in miR-338-expressing cells was restored to normal levels by the restoration of NRP1 expression. In vivo, miR-338 also decreased tumor growth and suppressed D-MVA by targeting NRP1. Therefore, we conclude that miR-338 acts as a novel tumor suppressor gene in gastric cancer. miR-338 can decrease migratory, invasive, proliferative and apoptotic behaviors, as well as gastric cancer EMT, by attenuating the expression of NRP1.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

GW20021F Anti-Fibronectin antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB1405825
Anti-FN1 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
AV41490
Anti-FN1 antibody produced in rabbit, affinity isolated antibody
HPA027066
Anti-FN1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2104810
Anti-FN1, (N-terminal) antibody produced in rabbit, affinity isolated antibody
SAB1406175
Anti-NELL1 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA051535
Anti-NELL1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1410833
Anti-NELL1 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
HPA030278
Anti-NRP1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1411572
Anti-NRP1 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
F0791 Monoclonal Anti-Fibronectin antibody produced in mouse, clone IST-3, ascites fluid
WH0004745M1
Monoclonal Anti-NELL1 antibody produced in mouse, clone 6A8, purified immunoglobulin, buffered aqueous solution
SAB1404116
Monoclonal Anti-NELL1 antibody produced in mouse, clone 3F1, purified immunoglobulin, buffered aqueous solution
N3287 Monoclonal Anti-Neuropilin-1 antibody produced in mouse, clone 130603, purified immunoglobulin, lyophilized powder
WH0008829M5
Monoclonal Anti-NRP1 antibody produced in mouse, clone 1B3, purified immunoglobulin, buffered aqueous solution