EMAIL THIS PAGE TO A FRIEND

Nucleic acids research

NuMA promotes homologous recombination repair by regulating the accumulation of the ISWI ATPase SNF2h at DNA breaks.


PMID 24753406

Abstract

Chromatin remodeling factors play an active role in the DNA damage response by shaping chromatin to facilitate the repair process. The spatiotemporal regulation of these factors is key to their function, yet poorly understood. We report that the structural nuclear protein NuMA accumulates at sites of DNA damage in a poly[ADP-ribose]ylation-dependent manner and functionally interacts with the ISWI ATPase SNF2h/SMARCA5, a chromatin remodeler that facilitates DNA repair. NuMA coimmunoprecipitates with SNF2h, regulates its diffusion in the nucleoplasm and controls its accumulation at DNA breaks. Consistent with NuMA enabling SNF2h function, cells with silenced NuMA exhibit reduced chromatin decompaction after DNA cleavage, lesser focal recruitment of homologous recombination repair factors, impaired DNA double-strand break repair in chromosomal (but not in episomal) contexts and increased sensitivity to DNA cross-linking agents. These findings reveal a structural basis for the orchestration of chromatin remodeling whereby a scaffold protein promotes genome maintenance by directing a remodeler to DNA breaks.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

06-598
Anti-acetyl-Histone H4 Antibody, 200 µg, Anti-acetyl-Histone H4 Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a & has been validated in ChIP, ICC, IP & WB.
07-478
Anti-SNF2β/BRG1 Antibody, 200 µL, Use Anti-SNF2β/BRG1 Antibody (rabbit polyclonal antibody) validated in IP, WB to detect SNF2β/BRG1 also known as Probable global transcription activator SNF2L4, ATP-dependent helicase SMARCA4, SNF2-beta, BRG-1.
SAB1406364
Anti-RAD51 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB2101936
Anti-RAD51 antibody produced in rabbit, affinity isolated antibody
SAB2700175
Anti-RAD51 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
HPA039310
Anti-RAD51 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1406602
Anti-XRCC2 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
X3878 Anti-XRCC2 antibody produced in rabbit, affinity isolated antibody, ammonium sulfate suspension
SAB2102727
Anti-XRCC2 antibody produced in rabbit, affinity isolated antibody
SAB1411401
Anti-XRCC2 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
WH0005888M1
Monoclonal Anti-RAD51 antibody produced in mouse, clone 2E5-E5, purified immunoglobulin, buffered aqueous solution
SAB1404293
Monoclonal Anti-RAD51 antibody produced in mouse, clone 4C11-B4, purified immunoglobulin, buffered aqueous solution
SAB1402394
Monoclonal Anti-XRCC2 antibody produced in mouse, clone 2H4, purified immunoglobulin, buffered aqueous solution