EMAIL THIS PAGE TO A FRIEND

PloS one

A role of Rab29 in the integrity of the trans-Golgi network and retrograde trafficking of mannose-6-phosphate receptor.


PMID 24788816

Abstract

Rab29 (also referred as Rab7L1) is a novel Rab protein, and is recently demonstrated to regulate phagocytosis and traffic from the Golgi to the lysosome. However, its roles in membrane trafficking have not been investigated extensively. Our results in this study revealed that Rab29 is associated with the trans-Golgi network (TGN), and is essential for maintaining the integrity of the TGN, because inhibition of the activity of Rab29 or depletion of Rab29 resulted in fragmentation of the TGN marked by TGN46. Expression of the dominant negative form Rab29T21N or shRNA-Rab29 also altered the distribution of mannose-6-phosphate receptor (M6PR), and interrupted the retrograde trafficking of M6PR through monitoring the endocytosis of CD8-tagged calcium dependent M6PR (cdM6PR) or calcium independent M6PR (ciM6PR), but without significant effects on the anterograde trafficking of vesicular stomatitis virus G protein (VSV-G). Our results suggest that Rab29 is essential for the integrity of the TGN and participates in the retrograde trafficking of M6PRs.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

HPA011332
Anti-IGF2R antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
GW21444 Anti-M6PR antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
AV43519
Anti-M6PR antibody produced in rabbit, IgG fraction of antiserum
SAB1307089
ANTI-M6PR(N-TERMINAL) antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
HPA026303
Anti-RAB29 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1406758
Anti-RAB7L1 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB1408646
Anti-RAB7L1 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB4503507
Anti-RAB7L1 antibody produced in rabbit, affinity isolated antibody
T9826
Anti-TGN38 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
T7576
Anti-TGN46 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
HPA012609
Anti-TGOLN2 antibody produced in rabbit, Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA012723
Anti-TGOLN2 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2103825
Anti-TGOLN2, (N-terminal) antibody produced in rabbit, affinity isolated antibody
CKOZFN17866 CompoZr® Knockout ZFN Kit, Human RAB7L1 (NM_001135662)
CKOZFND17866 CompoZr® Knockout ZFN Kit, ZFN plasmid only, Human RAB7L1 (NM_001135662)
SAB4700341 Monoclonal Anti-CD222 antibody produced in mouse, clone MEM-238, purified immunoglobulin, buffered aqueous solution
WH0008934M3
Monoclonal Anti-RAB7L1 antibody produced in mouse, clone 2B8, purified immunoglobulin, buffered aqueous solution
SAB1404654
Monoclonal Anti-RAB7L1 antibody produced in mouse, clone 1B10, purified immunoglobulin, buffered aqueous solution