EMAIL THIS PAGE TO A FRIEND

International journal of molecular medicine

Growth arrest-specific gene 6 protein promotes the proliferation and migration of endothelial progenitor cells through the PI3K/AKT signaling pathway.


PMID 24789534

Abstract

Endothelial progenitor cells (EPCs) play an important role in endothelial repair and vascular regeneration. Growth arrest-specific gene 6 (Gas6) is a novel key regulator of the vascular system, which is linked to a number of cardiovascular diseases. However, the effects of Gas6 on EPCs have not been elucidated to date. The present study was designed to determine the biological function of EPCs treated with Gas6 and to eludicate the underlying mechanisms. EPCs were isolated from umbilical cord blood and treated with various concentrations (25, 50, 100 and 200 ng/ml) of Gas6. The proliferation, migration and angiogenesis of the Gas6-treated EPCs were evaluated by MTT assay, Transwell assay and in vitro tube formation assay, respectively. The phosphorylation status of AKT and ERK was evaluated by western blot analysis. The results demonstrated that treatment with Gas6 enhanced the proliferation and migration of the EPCs in a dose-dependent manner. However, Gas6 did not promote the differentiation of EPCs on Matrigel. Gas6 induced the phosphorylation of AKT, but not that of ERK. The enhanced proliferation and migration induced by Gas6 was markedly suppressed by the inhibitor of PI3K but not by that of ERK. These results suggest that Gas6 activates the AKT signaling pathway, which, in turn, promotes the proliferation and migration of EPCs.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB4500797
Anti-AKT antibody produced in rabbit, affinity isolated antibody
SAB4500799
Anti-AKT antibody produced in rabbit, affinity isolated antibody
SAB4500800
Anti-AKT antibody produced in rabbit, affinity isolated antibody
SAB4500802
Anti-AKT antibody produced in rabbit, affinity isolated antibody
SAB4500803
Anti-AKT antibody produced in rabbit, affinity isolated antibody
SAB4300335
Anti-AKT1 (Ab-308) antibody produced in rabbit, affinity isolated antibody
SAB4300575
Anti-AKT1 (Ab-450) antibody produced in rabbit, affinity isolated antibody
SAB4300334
Anti-AKT1 (Ab-473) antibody produced in rabbit, affinity isolated antibody
GW22757 Anti-AKT1 (ab1) antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
GW22775 Anti-AKT1 (ab2) antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB2501934
Anti-AKT1 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB3500216
Anti-Akt1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
HPA002891
Anti-AKT1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV06008 Anti-AKT1 antibody produced in rabbit, affinity isolated antibody
SAB4500007
Anti-AKT1 antibody produced in rabbit, affinity isolated antibody
WH0000207M3
Monoclonal Anti-AKT1 antibody produced in mouse, clone 2E11, purified immunoglobulin, buffered aqueous solution
SAB1400005
Monoclonal Anti-AKT1 antibody produced in mouse, clone 6D6, purified immunoglobulin, buffered aqueous solution
SAB1400006
Monoclonal Anti-AKT1 antibody produced in mouse, clone 6G6, purified immunoglobulin, buffered aqueous solution
SAB1400007
Monoclonal Anti-AKT1 antibody produced in mouse, clone 2H1, purified immunoglobulin, buffered aqueous solution
SAB1400008
Monoclonal Anti-AKT1 antibody produced in mouse, clone 4B9, purified immunoglobulin, buffered aqueous solution
R2650 Monoclonal Anti-Rac antibody produced in mouse, clone 23A8, purified immunoglobulin, buffered aqueous solution
PLA0238
Rabbit anti-AKT1 Antibody, Affinity Purified, Powered by Bethyl Laboratories, Inc.