EMAIL THIS PAGE TO A FRIEND

Biochemical and biophysical research communications

Alpha-lipoic acid protects against cisplatin-induced ototoxicity via the regulation of MAPKs and proinflammatory cytokines.


PMID 24796665

Abstract

Cisplatin is an effective antineoplastic drug that is widely used to treat various cancers; however, it causes side effects such as ototoxicity via the induction of apoptosis of hair cells in the cochlea. Alpha-lipoic acid (ALA) has been reported to exert a protective effect against both antibiotic-induced and cisplatin-induced hearing loss. Therefore, this study was conducted to (1) elucidate the mechanism of the protective effects of ALA against cisplatin-induced ototoxicity using in vitro and ex vivo culture systems of HEI-OC1 auditory cells and rat cochlear explants and (2) to gain additional insight into the apoptotic mechanism of cisplatin-induced ototoxicity. ALA pretreatment significantly reduced apoptotic cell death of the inner and outer hair cells in cisplatin-treated organ of Corti explants and attenuated ototoxicity via marked inhibition of the increase in the expression of IL-1β and IL-6, the phosphorylation of ERK and p38, the degradation of IκBα, the increase in intracellular levels of ROS, and the activation of caspase-3 in cisplatin-treated HEI-OC1 cells. This study represents the first histological evaluation of the organ of Corti following treatment with ALA, and these results indicate that the protective effects of ALA against cisplatin-induced ototoxicity are mediated via the regulation of MAPKs and proinflammatory cytokines.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

M7556 Anti-MAP Kinase 2 (ERK-2) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL
HPA030069
Anti-MAPK1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB4300499
Anti-MAPK14 (Ab-182) antibody produced in rabbit, affinity isolated antibody
AV06004
Anti-MAPK14 antibody produced in rabbit, affinity isolated antibody
M0800
Anti-p38 MAP Kinase antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
SAB4500491
Anti-p38 MAPK antibody produced in rabbit, affinity isolated antibody
SAB4500492
Anti-p38 MAPK antibody produced in rabbit, affinity isolated antibody
SAB4500493
Anti-p38 MAPK antibody produced in rabbit, affinity isolated antibody
SAB4300200
Anti-phospho-MAPK14 (pThr180) antibody produced in rabbit, affinity isolated antibody
SAB4300201
Anti-phospho-MAPK14 (pTyr182) antibody produced in rabbit, affinity isolated antibody
P1491 Anti-phospho-p38 (pThr180/pTyr182) antibody produced in rabbit, affinity isolated antibody, buffered aqueous glycerol solution
SAB4504095
Anti-phospho-p38 MAPK (pThr179+Tyr181) antibody produced in rabbit, affinity isolated antibody
SAB4504497
Anti-phospho-p38 MAPK (pThr180) antibody produced in rabbit, affinity isolated antibody
SAB4504498
Anti-phospho-p38 MAPK (pTyr182) antibody produced in rabbit, affinity isolated antibody
SAB4504094
Anti-phospho-p38 MAPK (pTyr322) antibody produced in rabbit, affinity isolated antibody
SAB4502906
Anti-Synaptophysin antibody produced in rabbit, affinity isolated antibody
HPA002858
Anti-SYP antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2102352
Anti-SYP antibody produced in rabbit, affinity isolated antibody
PLA0236
Rabbit anti-ERK2 Antibody, Affinity Purified, Powered by Bethyl Laboratories, Inc.