EMAIL THIS PAGE TO A FRIEND

Analytical and bioanalytical chemistry

Quantitative determination of free and total bisphenol A in human urine using labeled BPA glucuronide and isotope dilution mass spectrometry.


PMID 24817354

Abstract

Bisphenol A (BPA) is a widely used industrial chemical in the manufacturing of polycarbonate plastic bottles, food and beverage can linings, thermal receipts, and dental sealants. Animal and human studies suggest that BPA may disrupt normal hormonal function and hence, potentially, have negative effects on the human health. While total BPA is frequently reported, it is recognized that free BPA is the biologically active form and is rarely reported in the literature. The objective of this study was to develop a sensitive and improved method for the measurement of free and total BPA in human urine. Use of a labeled conjugated BPA (bisphenol A-d6 β-D-glucuronide) allowed for the optimization of the enzymatic reaction and permitted an accurate determination of the conjugated BPA concentration in urine samples. In addition, a (13)C12-BPA internal standard was used to account for the analytical recoveries and performance of the isotope dilution method. Solid-phase extraction (SPE) combined with derivatization and analysis using a triple quadrupole GC-EI/MS/MS system achieved very low method detection limit of 0.027 ng/mL. BPA concentrations were measured in urine samples collected during the second and third trimesters of pregnancy in 36 Canadian women. Total maternal BPA concentrations in urine samples ranged from not detected to 9.40 ng/mL (median, 1.21 ng/mL), and free BPA concentrations ranged from not detected to 0.950 ng/mL (median, 0.185 ng/mL). Eighty-six percent of the women had detectable levels of conjugated BPA, whereas only 22 % had detectable levels of free BPA in their urine. BPA levels measured in this study agreed well with data reported internationally.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

442840
4,4′-Isopropylidenediphenol, analytical standard
C15H16O2
691275
Acetic anhydride, Lonza quality, ≥99.5% (GC)
C4H6O3
242845
Acetic anhydride, ACS reagent, ≥98.0%
C4H6O3
320102
Acetic anhydride, ReagentPlus®, ≥99%
C4H6O3
539996
Acetic anhydride, 99.5%
C4H6O3
33214
Acetic anhydride, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99% (GC)
C4H6O3
A6404
Acetic anhydride, reagent grade, ≥98%
C4H6O3
45840
Acetic anhydride
C4H6O3
01-0390
Acetic anhydride, SAJ first grade, ≥93.0%
C4H6O3
01-0380
Acetic anhydride, JIS special grade
C4H6O3
L095004
Acetic anhydride
C4H6O3
90872
Acetone, analytical standard
C3H6O
1006801
Acetone, United States Pharmacopeia (USP) Reference Standard
C3H6O
PHR1219
Acetone, Pharmaceutical Secondary Standard; Certified Reference Material
C3H6O
239658
Bisphenol A, ≥99%
C15H16O2
42088
Bisphenol A, certified reference material, TraceCERT®
C15H16O2
133027
Bisphenol A, 97%
C15H16O2
66742
Dichloromethane, Selectophore, ≥99.5%
CH2Cl2
02575
Dichloromethane, analytical standard
CH2Cl2
48538
Dichloromethane, analytical standard, stabilized
CH2Cl2
48600
Dichloromethane solution, certified reference material, 200 μg/mL in methanol
CH2Cl2
40042
Dichloromethane solution, certified reference material, 5000 μg/mL in methanol
CH2Cl2
52750
Hexane, analytical standard
C6H14
52765
Hexane, puriss. p.a., ACS reagent, ≥99.0% (GC)
C6H14
15671
Hexane, puriss., ≥95% (GC)
C6H14
M1550000
Methylene chloride, European Pharmacopoeia (EP) Reference Standard
CH2Cl2
PHR1557
Methylene Chloride, Pharmaceutical Secondary Standard; Certified Reference Material
CH2Cl2
M0789
N-Methyl-bis(trifluoroacetamide), for GC derivatization, ≥97.0% (GC)
C5H3F6NO2
M9513
N-Methyl-bis(trifluoroacetamide), ~95% (capillary GC)
C5H3F6NO2
65943
N-Methyl-bis(trifluoroacetamide), for GC derivatization, ≥99.0% (GC)
C5H3F6NO2