EMAIL THIS PAGE TO A FRIEND

International journal of cardiology

Anti-hypertrophic effects of oxytocin in rat ventricular myocytes.


PMID 24852833

Abstract

Oxytocin (OT) and functional OT receptor (OTR) are expressed in the heart and are involved in blood pressure regulation and cardioprotection. Cardiac OTR signaling is associated with atrial natriuretic peptide (ANP) and nitric oxide (NO) release. During the synthesis of OT, its precursor, termed OT-Gly-Lys-Arg (OT-GKR), is accumulated in the developing rat heart. Consequently, we hypothesized that an OT-related mechanism of ANP controls cardiomyocyte (CM) hypertrophy. The experiments were carried out in newborn and adult rat CM cultures. The enhanced protein synthesis and increased CM volume were mediated by a 24-h treatment with endothelin-1 or angiotensin II. The treatment of CM with OT or its abundant cardiac precursor, OT-GKR, revealed ANP accumulation in the cell peri-nuclear region and increased intracellular cGMP. Consequently, the CM hypertrophy was abolished by the treatment of 10nM OT or 10nM OT-GKR. The ANP receptor antagonist (anantin) and NO synthases inhibitor (l-NAME) inhibited cGMP production in CMs exposed to OT. STO-609 and compound C inhibition of anti-hypertrophic OT effects in CMs indicated the contribution of calcium-calmodulin kinase kinase and AMP-activated protein kinase pathways. Moreover, in ET-1 stimulated cells, OT treatment normalized the reduced Akt phosphorylation, prevented abundant accumulation of ANP and blocked ET-1-mediated translocation of nuclear factor of activated T-cells (NFAT) into the cell nuclei. cGMP/protein kinase G mediates OT-induced anti-hypertrophic response with the contribution of ANP and NO. OT treatment represents a novel approach in attenuation of cardiac hypertrophy during development and cardiac pathology.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB4500797
Anti-AKT antibody produced in rabbit, affinity isolated antibody
SAB4500799
Anti-AKT antibody produced in rabbit, affinity isolated antibody
SAB4500800
Anti-AKT antibody produced in rabbit, affinity isolated antibody
SAB4500802
Anti-AKT antibody produced in rabbit, affinity isolated antibody
SAB4500803
Anti-AKT antibody produced in rabbit, affinity isolated antibody
SAB4300335
Anti-AKT1 (Ab-308) antibody produced in rabbit, affinity isolated antibody
SAB4300575
Anti-AKT1 (Ab-450) antibody produced in rabbit, affinity isolated antibody
SAB4300334
Anti-AKT1 (Ab-473) antibody produced in rabbit, affinity isolated antibody
SAB3500216
Anti-Akt1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
HPA002891
Anti-AKT1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV06008 Anti-AKT1 antibody produced in rabbit, affinity isolated antibody
SAB4500007
Anti-AKT1 antibody produced in rabbit, affinity isolated antibody
SAB2100665
Anti-EIF3G antibody produced in rabbit, affinity isolated antibody
HPA041997
Anti-EIF3G antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV40486
Anti-EIF3S4 antibody produced in rabbit, IgG fraction of antiserum
AV38046
Anti-GTF2H4 (AB1) antibody produced in rabbit, IgG fraction of antiserum
AV38047
Anti-GTF2H4 (AB2) antibody produced in rabbit, IgG fraction of antiserum
AV32598
Anti-GTF2H4 antibody produced in rabbit, affinity isolated antibody
SAB2100990
Anti-GTF2H4 antibody produced in rabbit, affinity isolated antibody
SAB4300493
Anti-MAPK3 (Ab-202) antibody produced in rabbit, affinity isolated antibody
SAB4300494
Anti-MAPK3 (Ab-204) antibody produced in rabbit, affinity isolated antibody
SAB2701851
Anti-mapk3 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2701770
Anti-mapk3 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
HPA003995
Anti-MAPK3 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA005700
Anti-MAPK3 antibody produced in rabbit, Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2104574
Anti-MAPK3 antibody produced in rabbit, affinity isolated antibody
SAB2104732
Anti-MAPK3 antibody produced in rabbit, affinity isolated antibody
M3946 Anti-MNK1 antibody produced in rabbit, ~1.4 mg/mL, affinity isolated antibody, buffered aqueous solution
SAB4503422
Anti-MNK1 antibody produced in rabbit, affinity isolated antibody
SAB4503423
Anti-MNK1 antibody produced in rabbit, affinity isolated antibody